ATI RN
microbiology chapter 10 test bank Questions
Question 1 of 9
Ziehl-Neelsen staining is for detection of:
Correct Answer: A
Rationale: Step 1: Ziehl-Neelsen staining is a type of acid-fast staining used to detect acid-resistant bacteria like Mycobacterium species. Step 2: Acid-resistant bacteria have a high lipid content in their cell walls, making them resist decolorization with acid-alcohol. Step 3: Mycobacterium species, such as M. tuberculosis and M. leprae, are examples of acid-resistant bacteria. Step 4: Spores are not typically stained using Ziehl-Neelsen staining, making choice B incorrect. Step 5: Metachromatic bodies are stained using specific techniques like Albert's stain, not Ziehl-Neelsen staining, making choice C incorrect. Summary: Ziehl-Neelsen staining is specifically used for acid-resistant bacteria detection, making choice A the correct answer. Choices B and C are incorrect as they do not align with the purpose of Ziehl-Neelsen staining. Choice D is incorrect as Ziehl
Question 2 of 9
Presence of pathogenic microorganisms in the air can be detected by presence of sanitary representative bacteria. Choose bacteria that are indicators of direct epidemiological danger:
Correct Answer: A
Rationale: The correct answer is A: Hemolytic streptococci. Hemolytic streptococci are pathogenic bacteria that can cause severe infections in humans, indicating direct epidemiological danger. Hemolytic streptococci are commonly found in the respiratory tract and can be transmitted through the air, making them a good indicator of pathogenic microorganisms in the air. Summary: - B: Sarcina, molds, yeast, and Micrococci are not typically indicators of direct epidemiological danger related to pathogenic microorganisms in the air. - Hemolytic streptococci, on the other hand, are pathogenic bacteria that can cause serious infections and are commonly transmitted through the air, making them a suitable indicator in this context.
Question 3 of 9
The cell organelle responsible for the packaging of proteins is the:
Correct Answer: B
Rationale: The correct answer is B: ribosome. Ribosomes are responsible for protein synthesis, not packaging. The Golgi complex (A) processes and packages proteins. The rough endoplasmic reticulum (rER) (C) is involved in protein synthesis and transport. Lysosomes (D) contain enzymes for digestion, not protein packaging. Therefore, ribosomes are the organelle directly involved in protein synthesis, making it the correct choice.
Question 4 of 9
Which one is NOT included in the non-specific immune response
Correct Answer: B
Rationale: Memory cells are not part of the non-specific immune response because they are a component of the specific immune response. Non-specific immune responses are immediate and target a broad range of pathogens, whereas memory cells are formed after exposure to a specific pathogen to provide a quicker and stronger response upon subsequent exposure. Lysozyme (A), Interferon (C), and Phagocytosis (D) are all part of the non-specific immune response. Lysozyme is an enzyme that destroys bacterial cell walls, interferon helps to inhibit viral replication, and phagocytosis involves the engulfing and destruction of pathogens by phagocytes. These responses are not specific to particular pathogens and are the body's first line of defense.
Question 5 of 9
The bacterial species that is resistant to novobiocin is:
Correct Answer: C
Rationale: The correct answer is C: Staphylococcus saprophyticus. The rationale is that Staphylococcus saprophyticus is the only one among the listed species that is known to be resistant to novobiocin. This resistance is due to the specific mechanism of action of novobiocin, which targets DNA gyrase, and Staphylococcus saprophyticus possesses an altered DNA gyrase enzyme that renders it resistant to the antibiotic. Staphylococcus aureus and Staphylococcus epidermidis are susceptible to novobiocin, making choices A and B incorrect. Choice D, "none of the listed," is also incorrect as Staphylococcus saprophyticus is indeed resistant to novobiocin.
Question 6 of 9
Tularemia is most often a:
Correct Answer: B
Rationale: The correct answer is B: Zoonosis. Tularemia is primarily a zoonotic disease, meaning it is transmitted from animals to humans. The bacterium that causes tularemia, Francisella tularensis, commonly infects animals like rabbits, rodents, and ticks, which serve as reservoir hosts. Humans can get infected through direct contact with infected animals, insect bites, or contaminated water or soil. This zoonotic nature of tularemia distinguishes it from anthroponosis (A), which is transmitted only between humans, and sapronosis (C), which is transmitted through non-living environmental sources. Choice D is incorrect because there is a correct answer, which is zoonosis.
Question 7 of 9
Microscopic examination of pus from a wound revealed Gram-positive cocci in grape-like clusters. The bacteria were catalase- and coagulase-positive. Identify the microorganism.
Correct Answer: A
Rationale: Step 1: Gram-positive cocci in grape-like clusters suggest Staphylococcus. Step 2: Catalase-positive indicates Staphylococcus (negative for Streptococcus). Step 3: Coagulase-positive is specific for Staphylococcus aureus. Summary: B: Streptococcus pyogenes is catalase-negative. C: Enterococcus faecalis does not form grape-like clusters. D: Micrococcus luteus is catalase-positive but not coagulase-positive.
Question 8 of 9
A patient with a wound infection had Gram-positive cocci in clusters isolated from a wound culture. The organism was catalase-positive and coagulase-positive. What is the likely causative agent?
Correct Answer: A
Rationale: 1. Gram-positive cocci in clusters suggest Staphylococcus species. 2. Catalase-positive and coagulase-positive characteristics match with Staphylococcus aureus. 3. Staphylococcus aureus is a common cause of wound infections due to its virulence factors. 4. Other choices are incorrect: B) S. epidermidis is coagulase-negative and a common contaminant, C) S. pyogenes is catalase-negative, D) Enterococcus faecalis is not typically associated with wound infections.
Question 9 of 9
A puncture sample has been taken from the inguinal lymph nodes of a patient provisionally diagnosed with plague. The sample was inoculated into a hard nutrient medium. What shape will the colonies have, if the diagnosis is confirmed?
Correct Answer: B
Rationale: The correct answer is B: Shagreen leather. Yersinia pestis, the bacterium causing plague, forms colonies resembling shagreen leather on a hard nutrient medium due to its distinctive morphology. This morphology includes a rough, irregular, and raised appearance, similar to the texture of shagreen leather. A: Dewdrops - Incorrect. Dewdrops do not accurately describe the colony morphology of Yersinia pestis. C: Lace handkerchief - Incorrect. Lace handkerchief does not accurately describe the colony morphology of Yersinia pestis. D: Mercury drops - Incorrect. Mercury drops do not accurately describe the colony morphology of Yersinia pestis.