microbiology chapter 11 test bank -Nurselytic

Correct Answer: B

Rationale: The correct answer is B: Bergey's Manual of Systematic Bacteriology. This manual is a comprehensive and authoritative resource specifically dedicated to bacterial taxonomy, classification, and identification. It provides detailed descriptions and classification schemes for bacteria, aiding in accurate identification and understanding of bacterial species. Gray's Anatomy (choice
A) focuses on human anatomy, the WHO Manual (choice
C) focuses on global health guidelines, and Darwin's Origin of Species (choice
D) discusses evolution and natural selection, none of which are specialized taxonomic resources for bacteria.

Correct Answer: A

Rationale: The correct answer is A: Agglutination. Agglutination is commonly used to identify Vibrio cholerae by clumping together the bacteria with specific antibodies. This reaction occurs when antibodies bind to antigens on the bacterial surface, leading to visible clumps. In contrast, Precipitation, Western blot, and ELISA are not typically used to identify Vibrio cholerae. Precipitation involves antigen-antibody complexes becoming insoluble, Western blot is used for protein detection, and ELISA is commonly used for detecting specific antigens or antibodies in a sample. Agglutination is the most appropriate choice for identifying Vibrio cholerae due to its specific interaction with the bacteria's surface antigens.

Correct Answer: A

Rationale: Rationale for Correct Answer A (Lamblia):
1. Pyriform protozoa with twin nuclei, four pairs of flagella, and a suctorial disc match the characteristics of Giardia lamblia.
2. The presence of two supporting filaments between the nuclei is a unique feature of Giardia lamblia.
3. Giardia lamblia is known to infect the duodenum and cause symptoms such as diarrhea and abdominal pain.

Summary of Incorrect

Choices:
B:
Toxoplasma -
Toxoplasma gondii is not characterized by four pairs of flagella or supporting filaments in the nuclei.
C: Leishmania - Leishmania species do not typically have the described morphology or location in the duodenum.
D: Intestinal trichomonad - Intestinal trichomonads do not exhibit the specific features mentioned in the question, such as twin nuclei and supporting filaments.

Correct Answer: D

Rationale: The correct answer is D: Moeller. Moeller staining method is specifically designed to detect spores due to its ability to differentiate between the spore and the vegetative cell. It involves applying multiple stains such as malachite green, safranin, and heat to visualize the endospore structure. This method is essential for proving the formation of spores as it highlights the resistant spore structures within the bacterial cell.

A: Klett and Gins is not used for spore staining.
B: Ziehl-Neelsen is used for acid-fast staining, not spore staining.
C: Gram and Loeffler's are used for gram staining, not spore staining.

Correct Answer: D

Rationale: The correct answer is D: Cell cultures. Rotavirus diagnosis involves detecting viral antigens in stool samples using cell cultures. Cell cultures allow for the replication of the virus, facilitating its identification. A: Egg inoculation and B: Sensitive laboratory animals are not commonly used for Rotavirus diagnosis due to ethical concerns and practical limitations. C: Ascoli test is used for detecting specific proteins in serum, not for Rotavirus detection in stool samples. In summary, cell cultures are the most appropriate method for laboratory diagnosis of Rotavirus infection due to their ability to support viral replication and antigen detection.