Which staining method is used for proving the formation of spores

Correct Answer: D

Rationale: The correct answer is D: Moeller. Moeller staining method is specifically designed to detect spores due to its ability to differentiate between the spore and the vegetative cell. It involves applying multiple stains such as malachite green, safranin, and heat to visualize the endospore structure. This method is essential for proving the formation of spores as it highlights the resistant spore structures within the bacterial cell. A: Klett and Gins is not used for spore staining. B: Ziehl-Neelsen is used for acid-fast staining, not spore staining. C: Gram and Loeffler's are used for gram staining, not spore staining.

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. This bacterium fits all the characteristics mentioned: Gram-positive rods with spore formation, motile, and gas production in nutrient broth. Clostridium perfringens is known to cause gas gangrene, which aligns with the gas production in nutrient broth. Bacillus anthracis (B) causes anthrax and does not typically produce gas. Clostridium tetani (C) causes tetanus and does not form spores that are motile. Bacillus cereus (D) is a common cause of food poisoning and does not cause gas gangrene.

Correct Answer: B

Rationale: The correct answer is B: Rickettsia rickettsii. Rocky Mountain spotted fever is caused by Rickettsia rickettsii, a bacterium transmitted by ticks. This pathogen primarily affects the endothelial cells of blood vessels, leading to vasculitis. Borrelia burgdorferi (choice A) causes Lyme disease, not Rocky Mountain spotted fever. Rickettsia prowazekii (choice C) causes epidemic typhus, and Ehrlichia chaffeensis (choice D) causes human monocytic ehrlichiosis, both of which are distinct from Rocky Mountain spotted fever. Therefore, the correct choice is B based on the specific etiology of the disease.

Correct Answer: D

Rationale: The correct answer is D: IgM. IgM antibodies are the first type of antibodies produced by the immune system in response to an acute infection, such as Parvovirus B19. During the first 10-14 days of the infection, IgM antibodies are usually detectable in the blood, indicating a recent or ongoing infection. IgG antibodies (choice A) are produced later in the immune response and indicate past infection or immunity. IgA (choice B) is more commonly associated with mucosal immunity and may not be as prominent in the early stages of systemic infections. IgD (choice C) is primarily found on the surface of B cells and is involved in B cell activation but is not typically used as a marker for acute infections like IgM.

Correct Answer: C

Rationale: The correct answer is C because Leptospira cannot be cultivated on blood and chocolate agar. The bacterium requires specialized media such as Ellinghausen-McCullough-Johnson-Harris (EMJH) medium for growth. Blood and chocolate agar do not provide the necessary nutrients for the bacterium's growth. Leptospira is a fastidious organism that has unique culture requirements. Therefore, the statement that Leptospira can be cultivated on blood and chocolate agar is incorrect. The other choices are correct: A - Leptospirosis is an acute infectious disease with a typical geographic distribution, B - Reservoirs of leptospira are different wild and domestic animals, mainly rodents, and D - Doxycycline and penicillins are drugs of choice for the treatment of leptospirosis.

Correct Answer: A

Rationale: The correct answer is A: Neutralization. In this experiment, the researchers used antitoxic antibotulinic serum of different types to neutralize the botulinic toxin in tinned meat extract injected into mice. Neutralization is the process of rendering a toxin harmless by combining it with specific antibodies. The purpose of injecting the antitoxic serum was to neutralize the toxin and prevent it from causing harm to the mice. This serological reaction of neutralization directly aligns with the objective of the experiment. Summary of other choices: B: Precipitation - Precipitation involves the formation of a visible insoluble complex when an antigen reacts with its corresponding antibody. This process was not described in the scenario. C: Complement binding - Complement binding involves the activation of the complement system by antigen-antibody complexes. This was not the main focus of the experiment described. D: Double immune diffusion - This technique is used to detect the presence of specific antigens or antibodies

Correct Answer: A

Rationale: The correct answer is A: Logarithmic phase. During this phase, bacteria multiply exponentially due to abundant nutrients and favorable conditions. The growth is rapid and follows a geometric progression. In contrast, the Death phase (choice B) occurs when nutrients are depleted, leading to a decline in bacterial numbers. The Stationary phase (choice C) is when growth equals death rates, resulting in a stable population size. Choice D is incorrect because the Logarithmic phase accurately describes the exponential growth pattern of bacteria in artificial media.

Correct Answer: A

Rationale: The correct answer is A: Toxoplasma. Toxoplasma gondii causes Toxoplasmosis, a protozoan disease that can affect the brain and lead to vision loss. The halfmoon-shaped unicellular organisms with pointed ends described in the blood analysis are characteristic of Toxoplasma parasites. Toxoplasma infection commonly occurs through ingestion of contaminated food or water, or contact with infected cat feces. Leishmania causes Leishmaniasis, Lamblia causes Giardiasis, Amoeba causes Amebiasis, and Trichomonad causes Trichomoniasis, none of which match the symptoms and blood analysis findings described in the question.

Correct Answer: B

Rationale: The correct answer is B: Staphylococcus aureus. This bacterium is known as a common cause of hospital-acquired infections due to its ability to colonize the skin and mucous membranes of healthcare workers and patients. Staphylococcus aureus can cause various infections, including surgical site infections and bloodstream infections, in healthcare settings. Rationale: 1. Clostridium botulinum is known for causing foodborne botulism, not hospital-acquired infections. 2. Treponema pallidum causes syphilis, which is primarily transmitted through sexual contact, not hospital settings. 3. Mycobacterium leprae causes leprosy, which is not commonly associated with hospital-acquired infections. In summary, Staphylococcus aureus is the correct answer as it is a common cause of hospital-acquired infections due to its prevalence in healthcare environments, unlike the other choices which are not typically associated with hospital-acquired infections.