Which one of the methods is used to identify cytoplasmic inclusions?

Correct Answer: A

Rationale: The correct answer is A: Neisser staining. This method is specifically used to identify cytoplasmic inclusions by staining them with a specific dye that highlights these structures. Neisser staining is designed to selectively target and visualize cytoplasmic inclusions, making it the most appropriate choice for this purpose. Option B: Clett staining, Option C: Ziehl-Nielsen staining, and Option D: Gram staining are not typically used for identifying cytoplasmic inclusions. Clett staining is not a commonly known staining method for this purpose. Ziehl-Nielsen staining is primarily used to detect acid-fast bacteria like Mycobacterium tuberculosis. Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative based on cell wall characteristics. Therefore, these options are not suitable for identifying cytoplasmic inclusions.

Correct Answer: D

Rationale: The correct answer is D, "All of the above." Escherichia coli is the most common bacteria causing UTIs due to its ability to colonize the urinary tract. Salmonella enterica and Neisseria gonorrhoeae are not typically associated with UTIs. Therefore, by process of elimination, since A is correct, B and C are incorrect.

Correct Answer: D

Rationale: The correct answer is D because antigens can be composed of lipids, proteins, or polysaccharides. Antigens are molecules that can elicit an immune response in the body. They can be diverse in their chemical composition, leading to the recognition and response by the immune system. Therefore, the correct choice is D as antigens can be any of these chemical components. Choices A, B, and C are incorrect because they each represent a possible chemical composition of antigens, but the flexibility of antigens to be made up of any of these components makes D the most accurate choice.

Correct Answer: B

Rationale: The correct answer is B: ELISA test for IgM anti-HAV in serum and HAV-Ag in stools. This test is the most reliable for detecting acute hepatitis A infection because IgM antibodies are produced early in the infection, indicating an active infection. Detection of HAV antigen in stools also confirms active viral shedding. A: Western blot assay for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity. C: ELISA test for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity. D: None of the above is incorrect as option B is the most appropriate choice for detecting acute hepatitis A infection.

Correct Answer: B

Rationale: The correct answer is B: Jenner. Edward Jenner performed the first vaccination against smallpox in 1796 by using cowpox virus to immunize individuals. He observed that milkmaids who had contracted cowpox did not get smallpox, leading to the development of the smallpox vaccine. Pasteur is known for germ theory, Semmelweis for handwashing in healthcare, and Koch for identifying the causative agents of diseases. However, none of them performed the first smallpox vaccination like Jenner.

Correct Answer: B

Rationale: The correct answer is B: streptococci. This term refers to beadlike chains of cocci formed after cell division along a single axis. "Diplococci" (A) are pairs of cocci, "tetrads" (C) are groups of four cocci, and "sarcinae" (D) are cube-like groups of eight cocci. Streptococci is the most appropriate term for describing the specific arrangement mentioned in the question.

Correct Answer: B

Rationale: The correct answer is B: IgM. IgM antibodies are the first type of antibodies produced in response to an infection, indicating an active or recent infection. In the case of a rubella virus infection in a fetus, IgM antibodies can be detected in the umbilical cord blood, confirming the presence of the virus. IgA antibodies are mainly found in mucosal areas, IgG antibodies indicate past infection or immunity, and IgD antibodies play a role in B cell activation but are not typically detected in this context.

Correct Answer: A

Rationale: The correct answer is A: Giardia lamblia. This protozoan matches the description of the causative agent because it has two nuclei, four flagella, and a ventral adhesive disc. Giardia lamblia is commonly found in the duodenum and causes giardiasis. The other choices can be eliminated because Leishmania donovani causes visceral leishmaniasis, not the described symptoms. Entamoeba coli is a non-pathogenic amoeba found in the large intestine. Trypanosoma gambiense causes African trypanosomiasis and is transmitted by the tsetse fly, not matching the described characteristics.

Correct Answer: A

Rationale: The correct answer is A: Chrysophyta. Diatoms are classified under the division Chrysophyta due to their unique characteristics, such as possessing a cell wall made of silica (siliceous frustule) and containing chlorophyll a and c for photosynthesis. Chrysophyta includes golden-brown algae, which diatoms are a part of. Rhodophyta (B) includes red algae, Chlorophyta (C) includes green algae, and Phaeophyta (D) includes brown algae. These choices are incorrect as they do not align with the specific characteristics and classification of diatoms within the kingdom Protista.