Which of the following uses the enzyme horseradish peroxidase (HRP)?

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Pediatric Immunization Practice Questions Questions

Question 1 of 5

Which of the following uses the enzyme horseradish peroxidase (HRP)?

Correct Answer: D

Rationale: Both ELISA and Western blotting commonly use horseradish peroxidase (HRP) as an enzyme for signal detection.

Question 2 of 5

Which of the following best describes the location of the delta (δ)-chain locus in human T-cell receptors?

Correct Answer: D

Rationale: The delta (δ)-chain locus is located within the alpha (α)-locus on chromosome 14.

Question 3 of 5

Superantigens such as staphylococcal enterotoxins bind to certain TCR chains and to MHC class II molecules. MHC binding does not involve the peptide groove. T cells can then release extremely high levels of cytokines, which lead to toxic shock syndrome (TSS) when they appear in the blood.

Correct Answer: B

Rationale: Superantigens typically bind to TCR beta (β) chains and MHC class II, triggering massive cytokine release.

Question 4 of 5

CD4 molecules bind with MHC molecules and are found on (expressed by) cells. CD8 molecules bind with MHC molecules and are found on (expressed by) cells.

Correct Answer: C

Rationale: CD4 binds MHC class II on helper T cells; CD8 binds MHC class I on cytotoxic T cells.

Question 5 of 5

Class II MHC molecules accommodate peptides and thus be referred to analogously as a 'hotdog in a bun', in contrast to class I MHC molecules.

Correct Answer: C

Rationale: Class II MHC binds larger peptides (9-30 residues), resembling a 'hotdog in a bun,' unlike class I.

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