Correct Answer: B

Rationale: B cells develop in the bone marrow, not the thymus. Firstly, hematopoietic stem cells in the bone marrow give rise to B cells. They undergo maturation and selection processes in the bone marrow, not in the thymus. Therefore, choice B is false. On the other hand, choices A, C, and D are correct. A is true because B cells with high affinity to self-antigens undergo apoptosis to prevent autoimmunity. C is true since B cells synthesize antibodies which can be displayed on their surface as B cell receptors. D is true because B cells can undergo class switching to produce different types of antibodies based on the immune response requirements.

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. Clostridium perfringens is a gram-positive spore-forming bacillus commonly found in soil. It produces hemolysis on blood agar, as described in the question. Clostridium botulinum is also a gram-positive spore-forming bacillus, but it does not typically cause hemolysis. Staphylococcus aureus is a gram-positive cocci, not a bacillus. Pseudomonas aeruginosa is a gram-negative bacterium, not a gram-positive spore-forming bacillus.

Correct Answer: D

Rationale: The correct answer is D because Streptococci are Gram-positive bacteria, typically spherical in shape, and range from 0.5-1 µm in size. A is incorrect as streptococci are not rod-shaped and are usually smaller in size. B is incorrect as they are not Gram-negative and are not typically of such small size. C is incorrect as streptococci are not Gram-negative and are not usually rod-shaped. Thus, based on their characteristics, the correct answer is D.

Correct Answer: B

Rationale: The correct answer is B: The generation time of bacteria refers to the time for a cell to divide and double in number. This is because the generation time is the period required for one bacterial cell to undergo binary fission and divide into two identical daughter cells. This process involves DNA replication, followed by cell division, resulting in an increase in the number of bacterial cells. Incorrect choices: A: The time for DNA replication - While DNA replication is a crucial part of bacterial growth and reproduction, it does not solely define the generation time. C: The time for spore formation - Spore formation is a specific process in certain bacteria and is not synonymous with the generation time. D: The time for toxin production - Toxin production is an independent process in bacteria and does not directly relate to the generation time.

Correct Answer: A

Rationale: The correct answer is A: Isoniazid. When rifampicin is used alone, there is a risk of developing resistance. Isoniazid is commonly used in combination with rifampicin to prevent resistance by targeting different mechanisms in the bacteria. Isoniazid works by inhibiting mycolic acid synthesis, while rifampicin targets RNA polymerase. This combination therapy is known as the first-line treatment for tuberculosis. Choices B, C, and D are incorrect because they do not have the same mechanism of action as isoniazid and would not be effective in preventing rifampicin resistance.