ATI RN
mastering microbiology test bank Questions
Question 1 of 5
Which of the following statements about in vitro cultivation of viruses is true?
Correct Answer: B
Rationale: The correct answer is B because viruses can indeed be cultured on hen embryos, cell cultures, and experimental animals in vitro. This method allows for the replication and study of viruses outside the host organism. Option A is incorrect as viral cultivation is possible in laboratory settings. Option C is incorrect because while viruses can be grown on nutrient media, it is not the only method. Option D is incorrect as viruses can be cultured in various types of cell cultures, not just human embryonic ones.
Question 2 of 5
Which of the following is a folate antagonist?
Correct Answer: B
Rationale: The correct answer is B: Trimethoprim. Trimethoprim is a folate antagonist that inhibits dihydrofolate reductase, an enzyme essential for folate metabolism in bacteria. This disrupts bacterial DNA synthesis, leading to bacteriostatic effects. Methyl Penicillin (A) is a beta-lactam antibiotic that interferes with bacterial cell wall synthesis. Vancomycin (C) inhibits cell wall synthesis by binding to D-Ala-D-Ala terminus of cell wall precursors. Gentamicin (D) inhibits bacterial protein synthesis by binding to the 30S subunit of the bacterial ribosome.
Question 3 of 5
Which of the following bacteria are unable to synthesize ATP?
Correct Answer: A
Rationale: The correct answer is A: Chlamydia. Chlamydia lacks the essential enzymes for ATP synthesis and relies on host cell ATP. Enterococci, Rickettsia, and Staphylococci possess the necessary enzymes for ATP synthesis through cellular respiration. Chlamydia's inability to synthesize ATP makes it dependent on the host cell for energy production, distinguishing it from the other bacteria listed.
Question 4 of 5
Flagellin is:
Correct Answer: A
Rationale: Flagellin is a protein component of bacterial flagella. It is not a carbohydrate, staining method, or an organelle. The correct answer is A because flagellin is a structural protein that forms the flagellar filament. It helps in bacterial motility and pathogenicity. Therefore, the correct choice is A.
Question 5 of 5
Precipitation reaction can be converted into agglutination reaction by coating soluble antigen onto
Correct Answer: D
Rationale: The correct answer is D: All of these. Coating soluble antigen onto Bentonite particles, RBCs, or Latex particles can convert a precipitation reaction into an agglutination reaction. This is because soluble antigens bind to their specific antibodies, causing the particles to clump together, visible as agglutination. Bentonite particles, RBCs, and Latex particles all provide surfaces for the antigen-antibody interaction to occur, leading to agglutination. Therefore, all of these options are correct for converting precipitation reactions to agglutination reactions.