ATI RN
openstax microbiology test bank Questions
Question 1 of 9
Which of the following stains is generally used for the diagnosis of tuberculosis?
Correct Answer: A
Rationale: The correct answer is A: Acid-fast stain. This stain is used for diagnosing tuberculosis due to the unique cell wall composition of Mycobacterium tuberculosis, which resists decolorization by acid-alcohol. This allows the bacteria to retain the primary stain (carbol fuchsin) and appear red under a microscope. Gram stain (B) is not suitable for Mycobacterium tuberculosis as it is gram-positive but does not retain the crystal violet-iodine complex. Negative stain (C) is not used for bacterial identification. Simple stain (D) does not differentiate acid-fast bacteria like Mycobacterium tuberculosis.
Question 2 of 9
Which of the following organisms belongs in the group I category?
Correct Answer: C
Rationale: The correct answer is C: Helicobacter pylori. Group I organisms are classified based on their low infectious doses and potential for bioterrorism. H. pylori is known to cause gastric ulcers and is categorized as a Group I organism due to its ability to cause disease at low doses. A: Francisella tularensis is a Group A bioterrorism agent, not Group I. B: Clostridium difficile is not typically classified as a Group I organism. D: Bacillus anthracis is a Group A bioterrorism agent, not Group I.
Question 3 of 9
A patient with bacterial endocarditis had Gram-positive cocci in chains isolated from blood cultures. The organism was catalase-negative. What is the most likely causative agent?
Correct Answer: A
Rationale: The correct answer is A: Streptococcus pyogenes. This is because Streptococcus pyogenes is a Gram-positive cocci in chains bacterium that is catalase-negative, making it the most likely causative agent in this scenario. Streptococcus pyogenes is a common pathogen causing bacterial endocarditis. Summary of other choices: B: Staphylococcus aureus is a Gram-positive cocci bacterium but is catalase-positive, so it is unlikely in this case. C: Enterococcus faecalis is a Gram-positive cocci bacterium, but it is catalase-negative, which is not consistent with the given information. D: Micrococcus luteus is a Gram-positive cocci bacterium but is catalase-positive, making it an unlikely causative agent.
Question 4 of 9
A 70-year-old man has developed prosphetic stomatitis. Apart of this he was found to have an evident lesion of mouth corners. Microscopical examination revealed large ovoid gram-positive cells. What microorganisms are most likely to be the leading etiological agent of such a lesion?
Correct Answer: A
Rationale: The correct answer is A: Candida fungi. In the context of a 70-year-old man with angular cheilitis (lesion at the corners of the mouth) and stomatitis, the presence of large ovoid gram-positive cells points towards Candida species, which are yeast fungi. Candida is a common opportunistic pathogen that can cause oral thrush and angular cheilitis in immunocompromised individuals, such as elderly people. Candida fungi are known to appear as large ovoid cells under microscopic examination. Summary of other choices: B: Streptococci are gram-positive bacteria and are not typically associated with large ovoid cells seen in this case. C: Staphylococci are also gram-positive bacteria and are not known to present as large ovoid cells in this context. D: Neisseria are gram-negative bacteria and are not the usual etiological agents for oral lesions like angular cheilitis.
Question 5 of 9
The main virulence factor of Neisseria meningitidis is?
Correct Answer: A
Rationale: The correct answer is A: The polysaccharide capsule. The capsule of Neisseria meningitidis plays a crucial role in its virulence by preventing phagocytosis, allowing the bacteria to evade the host's immune system. This leads to increased survival and colonization in the host. Choice B: The beta lactamase is incorrect because it is an enzyme that breaks down beta-lactam antibiotics and does not directly contribute to the virulence of Neisseria meningitidis. Choice C: The erythrogenic toxin is incorrect as this toxin is produced by Streptococcus pyogenes, not Neisseria meningitidis. Choice D: None of the above is incorrect as the polysaccharide capsule is indeed a key virulence factor of Neisseria meningitidis.
Question 6 of 9
(+) ssRNA viruses that have been isolated from birds, cats, dogs, pigs, sheep, cows, and humans and are a major cause of gastroenteritis are the:
Correct Answer: B
Rationale: The correct answer is B: caliciviruses. Caliciviruses are known to be (+) ssRNA viruses that cause gastroenteritis in a wide range of animals, including birds, cats, dogs, pigs, sheep, cows, and humans. These viruses are highly contagious and can spread through contaminated food or water. Rotaviruses (A) are also a common cause of gastroenteritis, but they are not exclusively isolated from the mentioned animals. Adenoviruses (C) typically cause respiratory or eye infections, not gastroenteritis. Noroviruses (D) are also a major cause of gastroenteritis in humans, but they are not commonly found in animals other than humans.
Question 7 of 9
During a microbiology session, students observed flagellated protozoa with an undulating membrane in vaginal discharge samples. What is the causative agent?
Correct Answer: A
Rationale: The correct answer is A: Trichomonas vaginalis. Trichomonas vaginalis is a flagellated protozoa that causes the sexually transmitted infection trichomoniasis. The presence of an undulating membrane in vaginal discharge samples is characteristic of Trichomonas vaginalis. Giardia lamblia causes gastrointestinal illness, not vaginal infections. Trypanosoma brucei causes African sleeping sickness transmitted by tsetse flies. Leishmania donovani causes visceral leishmaniasis transmitted by sandflies.
Question 8 of 9
During fibergastroscopy a patient with ulcer disease of the stomach, the mucosal biopsy is taken from the area of an ulcer. Impression smear is prepared from biopsy material and stained by Gram method; the rest of biopsy material is tested for urease activity. Microscopy of the impression smear revealed gram- negative spiral-shaped microorganisms, urease activity test is positive. What bacteria were detected?
Correct Answer: A
Rationale: The correct answer is A: Helicobacter pylori. Rationale: 1. Gram-negative spiral-shaped microorganisms match the morphology of Helicobacter pylori. 2. Positive urease activity is a characteristic feature of Helicjsonacter pylori. 3. Helicobacter pylori is specifically associated with gastric ulcers and is a common pathogen in the gastrointestinal tract. Summary: Choice B, Campilobacter jejuni, is a gram-negative curved bacterium but is not typically associated with gastric ulcers or urease activity. Choice C, Treponema pallidum, is a spirochete bacterium causing syphilis, which is not related to gastric ulcers. Choice D, Spirilla minor, is a generic term for spiral-shaped bacteria and is not a specific pathogen associated with gastric ulcers or urease activity.
Question 9 of 9
Which of the following stains is generally used for the diagnosis of tuberculosis?
Correct Answer: A
Rationale: The correct answer is A: Acid-fast stain. This stain is used for diagnosing tuberculosis due to the unique cell wall composition of Mycobacterium tuberculosis, which resists decolorization by acid-alcohol. This allows the bacteria to retain the primary stain (carbol fuchsin) and appear red under a microscope. Gram stain (B) is not suitable for Mycobacterium tuberculosis as it is gram-positive but does not retain the crystal violet-iodine complex. Negative stain (C) is not used for bacterial identification. Simple stain (D) does not differentiate acid-fast bacteria like Mycobacterium tuberculosis.