ATI RN
Basic Immune System Quizlet Questions
Question 1 of 5
Which of the following restriction endonucleases produce fragments with blunt ends?
Correct Answer: C
Rationale: The correct answer is C: Smal. Smal is a restriction endonuclease that cuts DNA at specific sequences, producing fragments with blunt ends. Smal recognizes the sequence CCCGGG and cuts between the two C's on both strands, resulting in blunt-ended fragments. Explanation for incorrect choices: A: Sacl - Sacl produces sticky ends, not blunt ends. B: EcoRI - EcoRI produces sticky ends with overhangs. D: HindIII - HindIII also produces sticky ends with overhangs. In summary, Smal is the only enzyme among the choices that generates DNA fragments with blunt ends.
Question 2 of 5
Which enzyme catalyzes the following reaction? Gap 5' - G G-A-T-C-C—3' ⋮ ⋮ ⋮ ⋮ ⋮ 3' - C-C-T-A-G G—5' Gap 5' - G-G-A-T-C-C—3' ⋮ ⋮ ⋮ ⋮ ⋮ 3' - C-C-T-A-G-G—5'
Correct Answer: A
Rationale: The correct answer is A: DNA ligase. DNA ligase catalyzes the joining of two DNA strands by forming a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another. In the given reaction, the enzyme is needed to seal the nick in the DNA strands after the Okazaki fragments have been synthesized during DNA replication. Explanation of other choices: B: DNA endonuclease - Endonucleases cleave the phosphodiester bond within a polynucleotide chain, not join two DNA strands. C: None of the options are correct - This is incorrect as DNA ligase is the correct enzyme for this reaction. D: DNA polymerase 1 - DNA polymerase synthesizes new DNA strands by adding nucleotides to the 3' end of the growing chain, not joining two DNA strands.
Question 3 of 5
Which of the following is the best dietary advice to maximize the immune function in healthy people?
Correct Answer: D
Rationale: Failed to generate a rationale of 500+ characters after 5 retries.
Question 4 of 5
When assessing a client with autoimmune disorder,what signs should the nurse look for in the client?
Correct Answer: B
Rationale: Failed to generate a rationale of 500+ characters after 5 retries.
Question 5 of 5
In a client with AIDS,a CD4 cell count above ______ mm³ would indicate that antiretroviral therapy is being effective.
Correct Answer: C
Rationale: Failed to generate a rationale of 500+ characters after 5 retries.