Which of the following microorganisms are acid-fast?

Correct Answer: B

Rationale: The correct answer is B: Mycobacterium tuberculosis. Acid-fast bacteria have a unique cell wall component called mycolic acid, making them resistant to decolorization by acid-alcohol during staining. Mycobacterium tuberculosis is acid-fast, as it retains the stain due to its high lipid content. Streptococcus pyogenes (A), Clostridium perfringens (C), and Bacillus subtilis (D) do not possess mycolic acid in their cell walls, so they are not acid-fast.

Correct Answer: D

Rationale: The correct answer is D: BCG vaccination. BCG vaccination is recommended for individuals with a negative Mantoux test result, especially in countries where tuberculosis is prevalent. This vaccination can help provide protection against tuberculosis. Choice A is incorrect because repeating the Mantoux test in a month is unnecessary since the boy already had a negative reaction. Choice B is incorrect as serodiagnosis of tuberculosis is not the first-line approach for diagnosis, especially in this scenario. Choice C is incorrect because urgent isolation is not necessary based solely on the Mantoux test result. Isolation is typically reserved for individuals with active tuberculosis.

Correct Answer: D

Rationale: The correct answer is D, "All of the above." Escherichia coli is the most common bacteria causing UTIs due to its ability to colonize the urinary tract. Salmonella enterica and Neisseria gonorrhoeae are not typically associated with UTIs. Therefore, by process of elimination, since A is correct, B and C are incorrect.

Correct Answer: A

Rationale: The correct answer is A: Neisser staining. This method is specifically used to identify cytoplasmic inclusions by staining them with a specific dye that highlights these structures. Neisser staining is designed to selectively target and visualize cytoplasmic inclusions, making it the most appropriate choice for this purpose. Option B: Clett staining, Option C: Ziehl-Nielsen staining, and Option D: Gram staining are not typically used for identifying cytoplasmic inclusions. Clett staining is not a commonly known staining method for this purpose. Ziehl-Nielsen staining is primarily used to detect acid-fast bacteria like Mycobacterium tuberculosis. Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative based on cell wall characteristics. Therefore, these options are not suitable for identifying cytoplasmic inclusions.

Correct Answer: A

Rationale: The presence of acid-fast bacilli stained red against a blue background indicates mycobacteria, specifically Mycobacterium tuberculosis in this case. Ziehl-Neelsen staining is commonly used to detect tuberculosis. Diphtheria is caused by Corynebacterium diphtheriae, which does not stain with Ziehl-Neelsen. Pneumonia is a general term for lung infections caused by various pathogens. Leprosy is caused by Mycobacterium leprae, which requires special staining methods different from Ziehl-Neelsen.

Correct Answer: B

Rationale: The correct answer is B: Vibrio cholerae. Cholera is caused by Vibrio cholerae, a gram-negative bacterium that produces a toxin leading to severe watery diarrhea. Shigella dysenteriae causes dysentery, not cholera. Salmonella enterica causes salmonellosis, not cholera. Clostridium perfringens is associated with food poisoning, not cholera. In summary, Vibrio cholerae is the correct answer because it is the specific bacterium known to cause cholera, while the other choices are associated with different diseases.

Correct Answer: B

Rationale: The correct answer is B: Personal eyewash. Personal eyewash is designed for immediate flushing until the victim reaches a more suitable safety unit. It provides a controlled flow of water to safely flush eyes while transporting the victim. The emergency shower (A) is meant for full-body coverage, not just for the eyes. Handheld drench hose (C) may not provide adequate flushing pressure. Combination unit (D) may not be easily portable for immediate use.

Correct Answer: B

Rationale: The correct answer is B: Disinfection. Disinfection is not a method for food preservation but rather a process of destroying harmful microorganisms on surfaces. A: Pasteurization involves heating food to kill pathogens, preserving the food. C: Irradiation uses radiation to kill bacteria and parasites, extending shelf life. D: Ultrahigh-temperature pasteurization involves heating food at very high temperatures to extend shelf life. In summary, disinfection is not a method used to preserve food but rather to clean surfaces.

Correct Answer: A

Rationale: The correct answer is A: Ziehl-Neelsen staining method was applied. 1. Ziehl's solution is used as a primary stain in Ziehl-Neelsen staining for acid-fast organisms like Mycobacterium tuberculosis. 2. Sulfuric acid is used as a decolorizer to remove excess stain. 3. Methylene blue is not part of the Ziehl-Neelsen staining method. 4. Burri's, Gram's, and Peshkov's staining methods are not used for acid-fast staining.