Which of the following is used to enumerate and/or separate live cells that express an antigen, sorted by applying an electric charge to the stained cells?

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Pediatric Immunization NCLEX Questions Quizlet Questions

Question 1 of 5

Which of the following is used to enumerate and/or separate live cells that express an antigen, sorted by applying an electric charge to the stained cells?

Correct Answer: C

Rationale: FACS (fluorescence-activated cell sorting) uses fluorescence and electric charge to sort live cells based on antigen expression, unlike the other methods which do not involve cell sorting.

Question 2 of 5

Production of secreted antibodies (pAs site) involves a chain of amino acids with a stretch of charged (versus non charged) amino acids at the terminus, in comparison to membrane bound antibody production (pAm site).

Correct Answer: B

Rationale: Secreted antibodies have a shorter, charged COOH terminus compared to the longer, hydrophobic terminus of membrane-bound antibodies.

Question 3 of 5

Which of the following TCR genetic chains contains V and J segments, similar to genes for immunoglobulin kappa and lambda light chains?

Correct Answer: D

Rationale: Gamma (γ) and alpha (α) chains contain V and J segments, analogous to light chain genes.

Question 4 of 5

Which of the following molecules is necessary (not accessory) for antigen recognition and T cell activation?

Correct Answer: B

Rationale: CD3 is essential for TCR signaling and T cell activation, unlike the accessory roles of CD4 and CD8.

Question 5 of 5

MHC class I has three genes (HLA-A, HLA-B, & HLA-C). Which of the following is the correct order from most alleles (most polymorphic) to least alleles?

Correct Answer: C

Rationale: HLA-B is the most polymorphic, followed by HLA-A, then HLA-C.

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