Which of the following is a host-dependent bacterium?

Correct Answer: C

Rationale: The correct answer is C: Rickettsia. Rickettsia is a host-dependent bacterium because it requires living cells of a host organism to survive and replicate. This bacterium is an obligate intracellular pathogen, meaning it cannot grow outside of a host cell. A: Mycoplasma and B: Ureaplasma are not host-dependent as they can grow and reproduce in cell-free environments due to possessing their own unique cell wall structures. D: Micrococcus is a free-living bacterium that can survive and reproduce independently without relying on a host organism.

Correct Answer: A

Rationale: The correct answer is A: Β-lactamase. In ELISA, enzymes like alkaline phosphatase, horseradish peroxidase, and Β-galactosidase are commonly used as labels to detect the presence of specific molecules. Β-lactamase is not typically used in ELISA as it is an enzyme used to hydrolyze β-lactam antibiotics. Therefore, it does not serve the purpose of detecting specific molecules in ELISA. Alkaline phosphatase, horseradish peroxidase, and Β-galactosidase are preferred enzymes in ELISA due to their ability to produce a detectable signal when bound to specific molecules.

Correct Answer: B

Rationale: The correct answer is B: Agglutination reaction. This test is commonly used to detect specific antibodies or antigens. In this case, it can be used to detect plague antigens in the rat tissues or antibodies in the villagers. This test is rapid, sensitive, and can help establish the presence of the causative agent quickly. A: Ring precipitation reaction is not typically used for diagnosing plague and is more commonly used in other types of infections. C: Passive agglutination reaction is not specific for plague and is more commonly used for detecting specific antibodies in diseases like syphilis. D: Neutralization reaction is not typically used for diagnosing plague and is more commonly used to determine the presence of neutralizing antibodies in viral infections.

Correct Answer: B

Rationale: The correct answer is B, Helicobacter pylori. This bacterium is the main cause of peptic ulcers by damaging the protective lining of the stomach and duodenum. It secretes enzymes that weaken the mucosal barrier, allowing stomach acid to cause damage. Salmonella typhi (A) causes typhoid fever, not peptic ulcers. Shigella boydii (C) causes dysentery, not peptic ulcers. Salmonella enterica (D) causes food poisoning, not peptic ulcers. Therefore, the correct answer is B based on its direct association with peptic ulcer formation.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Clostridium botulinum is known for producing the neurotoxin causing botulism. 2. Botulinum toxin paralyzes muscles, leading to symptoms of botulism. 3. Clostridium tetani produces tetanus toxin causing muscle rigidity, not botulism. 4. Streptococcus pneumoniae causes pneumonia, not botulism. 5. Escherichia coli can cause food poisoning, but not botulism. Summary: Choice A is correct (Clostridium botulinum) due to its association with botulism and neurotoxin production. Choices B, C, and D are incorrect as they are associated with different diseases and toxins.

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. This bacterium fits all the characteristics mentioned: Gram-positive rods with spore formation, motile, and gas production in nutrient broth. Clostridium perfringens is known to cause gas gangrene, which aligns with the gas production in nutrient broth. Bacillus anthracis (B) causes anthrax and does not typically produce gas. Clostridium tetani (C) causes tetanus and does not form spores that are motile. Bacillus cereus (D) is a common cause of food poisoning and does not cause gas gangrene.

Correct Answer: D

Rationale: The correct answer is D (4) because biosafety levels range from 1 to 4, each corresponding to the level of containment required when handling different types of organisms. BSL-1 is for low-risk agents, while BSL-4 is for dangerous pathogens. Choice A (1) is incorrect as there are multiple levels, not just one. Choice B (2) and C (3) are incorrect as they do not account for all the levels of biosafety required for handling various organisms. Therefore, D is the correct answer as it encompasses the full range of biosafety levels.

Correct Answer: A

Rationale: The correct answer is A: Entamoeba histolytica. This is because it is the only choice that matches the description of cysts with four nuclei, characteristic of Entamoeba histolytica. Entamoeba histolytica is a protozoan parasite that causes amoebiasis, leading to symptoms like persistent diarrhea. The other choices can be eliminated for the following reasons: B: Balantidium coli - It is a ciliated protozoan that causes dysentery in humans but does not produce cysts with four nuclei. C: Giardia lamblia - It is a flagellated protozoan that causes giardiasis, but it forms cysts with only two nuclei. D: Trichomonas hominis - It is a flagellated protozoan found in the human intestine, but it does not produce cysts with four nuclei. Therefore, based on the description of cysts with four nuclei in the stool sample,

Correct Answer: D

Rationale: The correct answer is D: Salmonella typhi. Typhoid fever is caused by the bacterium Salmonella typhi. This bacterium is responsible for causing the systemic infection that leads to symptoms such as high fever, abdominal pain, and diarrhea. Yersinia pestis (choice A) causes plague, Shigella dysenteriae (choice B) causes dysentery, and Escherichia coli (choice C) can cause various gastrointestinal infections, but not typhoid fever. Salmonella typhi specifically targets the intestines and is transmitted through contaminated food or water. Therefore, choice D is the correct answer for the cause of typhoid fever.