Which of the following growth media is most suitable for isolation of Candida:

Correct Answer: C

Rationale: Rationale: 1. Candida is a fungus commonly isolated from clinical specimens. 2. Sabouraud agar is the most suitable medium for Candida due to its low pH and high dextrose content. 3. Low pH inhibits bacterial growth, while dextrose promotes fungal growth. 4. Zeisler media, Chocolate agar, and Muller-Hinton agar are not specific for fungal isolation. Summary: - Zeisler media is used for anaerobic bacteria. - Chocolate agar is used for fastidious organisms like Haemophilus. - Muller-Hinton agar is for antibiotic susceptibility testing, not fungal isolation.

Correct Answer: C

Rationale: Rationale: 1. Candida is a fungus commonly isolated from clinical specimens. 2. Sabouraud agar is the most suitable medium for Candida due to its low pH and high dextrose content. 3. Low pH inhibits bacterial growth, while dextrose promotes fungal growth. 4. Zeisler media, Chocolate agar, and Muller-Hinton agar are not specific for fungal isolation. Summary: - Zeisler media is used for anaerobic bacteria. - Chocolate agar is used for fastidious organisms like Haemophilus. - Muller-Hinton agar is for antibiotic susceptibility testing, not fungal isolation.

Correct Answer: C

Rationale: The correct answer is C: Janssen. In the 16th century, Hans and Zacharias Janssen were a father-and-son team from the Netherlands who are credited with inventing the compound microscope. They constructed a simple tube with lenses at each end, which allowed for magnification of small objects. Their invention significantly contributed to the development of microscopy. Van Leeuwenhoek is known for his contributions to microscopy but did not invent the compound microscope. Semmelweis is known for his work in handwashing and preventing infection. Hooke made significant contributions to microscopy but did not invent the compound microscope.

Correct Answer: D

Rationale: The correct answer is D: Shigella dysenteriae I. This bacterium is known to produce Shiga toxin, which is responsible for the most severe bacillary infections. The toxin can cause bloody diarrhea and potentially life-threatening complications like hemolytic uremic syndrome. Shigella boydii, flexneri, and sonnei, while also causing bacillary dysentery, do not produce the same level of severity as Shigella dysenteriae I due to the absence of Shiga toxin.

Correct Answer: B

Rationale: Step-by-step rationale: 1. Fimbriae are hair-like structures on bacteria, not rope-like. 2. Fimbriae are smaller in diameter compared to flagella. 3. Fimbriae are used for adherence or conjugation, not flagella. Therefore, choice B is correct as it incorrectly describes fimbriae, while choices A, C, and D correctly characterize fimbriae.

Correct Answer: B

Rationale: The correct answer is B: Aminoglycosides. Aminoglycosides are antibiotics that act by binding to the 30S ribosomal subunit, disrupting protein synthesis in bacteria. This interaction affects the ribosomes directly, leading to inhibition of bacterial protein production. Penicillins (A) work by inhibiting cell wall synthesis, Sulfonamides (C) target folic acid synthesis, and Quinolones (D) inhibit DNA gyrase. These mechanisms do not directly affect the ribosomes, making them incorrect choices in this context.

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. On Endo agar, lactose-fermenting bacteria produce red colonies due to acid production. Escherichersia coli is a lactose-fermenting, gram-negative bacillus commonly found in the intestines. Salmonella and Shigella are lactose-negative and do not produce red colonies on Endo agar. Proteus is also lactose-negative and doesn't typically grow on Endo agar. Therefore, based on the lactose fermentation and colony color, Escherichia coli is the most likely microorganism present in the fecal sample.

Correct Answer: A

Rationale: Rationale: 1. Listeria monocytogenes is a Gram-positive bacterium known for causing foodborne illnesses. 2. Gram-negative bacteria have a different cell wall structure than Gram-positive bacteria, making choice A correct. 3. Choice B is incorrect as Listeria monocytogenes is not encapsulated or sporulating. 4. Choice C is incorrect as Listeria monocytogenes does have a cell wall. 5. Choice D is incorrect as Listeria monocytogenes is indeed a Gram-positive coccobacilli.

Correct Answer: B

Rationale: The correct answer is B: CDC. The Centers for Disease Control and Prevention (CDC) is responsible for establishing hand-washing guidelines in healthcare settings to prevent infections. CDC is a leading authority in public health and provides evidence-based recommendations. OSHA (A) focuses on workplace safety, NIAD (C) is not a recognized organization, and WHO (D) provides global health guidelines but CDC is the primary authority for hand hygiene in the US healthcare system.