Which of the following growth media is most suitable for isolation of Candida:

Correct Answer: C

Rationale: Rationale: 1. Candida is a fungus commonly isolated from clinical specimens. 2. Sabouraud agar is the most suitable medium for Candida due to its low pH and high dextrose content. 3. Low pH inhibits bacterial growth, while dextrose promotes fungal growth. 4. Zeisler media, Chocolate agar, and Muller-Hinton agar are not specific for fungal isolation. Summary: - Zeisler media is used for anaerobic bacteria. - Chocolate agar is used for fastidious organisms like Haemophilus. - Muller-Hinton agar is for antibiotic susceptibility testing, not fungal isolation.

Correct Answer: C

Rationale: The correct answer is C: Clostridium perfringens. This bacterium is the causative agent of gas gangrene due to its ability to produce gas and toxins in anaerobic conditions. Clostridium tetani causes tetanus, Clostridium botulinum causes botulism, and Bacillus anthracis causes anthrax, not gas gangrene. Clostridium perfringens stands out as the correct choice based on its specific association with gas gangrene and its pathogenic mechanisms.

Correct Answer: C

Rationale: Step 1: Anthrax causative agent is Bacillus anthracis, which forms spores. Step 2: Spores are highly resistant structures that can survive for a long time. Step 3: These spores can germinate into active bacteria when conditions are favorable. Step 4: The presence of spores in the powder makes it dangerous even long after being mailed. Summary: A: Incorrect - Anthrax does not form a protein capsule. B: Incorrect - Anthrax does not form a polysaccharide capsule. C: Correct - Anthrax forms spores that are highly resistant. D: Incorrect - Anthrax does not form flagella.

Correct Answer: C

Rationale: The correct answer is C, Meat-peptone agar, as it provides a simple nutritive media for bacterial growth. This agar contains meat extract and peptone, which are good sources of nutrients for bacterial growth. Glucose broth (A) is a liquid medium with glucose but lacks other essential nutrients. Ascites (serum) agar (B) is a selective medium used for detecting pathogens in clinical samples. Blood agar (D) is a differential medium used to distinguish different types of bacteria based on hemolytic reactions. Meat-peptone agar is the simplest and most general-purpose medium among the options provided.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Mycobacterium tuberculosis is the causative agent of tuberculosis. 2. Mycobacterium tuberculosis is a slow-growing, acid-fast bacterium. 3. It primarily infects the lungs and can spread to other parts of the body. 4. Bacillus anthracis causes anthrax, Clostridium tetani causes tetanus, and Treponema pallidum causes syphilis. 5. Therefore, choice A, Mycobacterium tuberculosis, is the correct answer for the bacteria responsible for tuberculosis.

Correct Answer: A

Rationale: The correct answer is A: BCG vaccine. Step 1: BCG vaccine is specifically designed to protect against tuberculosis, making it the appropriate choice for newborns. Step 2: STI vaccine is for sexually transmitted infections, not tuberculosis. Step 3: EV vaccine is not a recognized vaccine. Step 4: DPT vaccine protects against diphtheria, pertussis, and tetanus, not tuberculosis. In summary, BCG vaccine is the correct choice as it targets tuberculosis specifically, while the other options are designed for different purposes.

Correct Answer: A

Rationale: The correct answer is A: Shigella dysenteriae. It is the likely causative agent because it is a non-lactose-fermenting, Gram-negative rod commonly associated with severe diarrhea. Shigella dysenteriae specifically causes bacillary dysentery. Other choices are incorrect because: B: Escherichia coli is a lactose-fermenting bacterium commonly found in the gut and not typically associated with severe diarrhea. C: Salmonella typhi causes typhoid fever, not severe diarrhea. D: Proteus mirabilis is a urease-positive bacterium associated with urinary tract infections, not severe diarrhea.

Correct Answer: A

Rationale: The correct answer is A: Metronidazole. Amebic dysentery is caused by the parasite Entamoeba histolytica, not a bacterium, hence antibiotics like Chloramphenicol or Phthalazole are ineffective. Metronidazole is the drug of choice for treating amebic dysentery due to its efficacy against anaerobic organisms like Entamoeba histolytica. Furasolidone is used for treating bacterial infections, not parasitic ones like amebiasis. The patient's symptoms of diarrhea, stomach aches, and a recent history of drinking water from a potentially contaminated source in the forest are indicative of a parasitic infection, supporting the choice of Metronidazole for treatment.

Correct Answer: D

Rationale: The correct answer is D: Shigella dysenteriae I. This bacterium is known to produce Shiga toxin, which is responsible for the most severe bacillary infections. The toxin can cause bloody diarrhea and potentially life-threatening complications like hemolytic uremic syndrome. Shigella boydii, flexneri, and sonnei, while also causing bacillary dysentery, do not produce the same level of severity as Shigella dysenteriae I due to the absence of Shiga toxin.