Which of the following enzymes removes 5' phosphat

Question 1 of 5

Which of the following enzymes removes 5' phosphate groups thereby preventing or decreasing the self-sealing of restricted plasmid vector molecules?

A. Calf intestinal phosphatase B. Pyrophosphatase C. Phosphohydrolase D. phosphate exonuclease

Correct Answer: A

Rationale: The correct answer is A: Calf intestinal phosphatase. This enzyme removes 5' phosphate groups from DNA molecules, preventing self-ligation of restricted plasmid vectors. This step is crucial in generating linearized vectors for downstream applications. Pyrophosphatase (B) hydrolyzes pyrophosphate bonds, not 5' phosphate groups. Phosphohydrolase (C) is a general term for enzymes that hydrolyze phosphoester bonds, not specific to 5' phosphate removal. Phosphate exonuclease (D) degrades nucleic acids by removing nucleotides from the 3' end, not the 5' phosphate group.

Question 2 of 5

Which of the following should a client with autoimmune disorder be advised to avoid?

A. Rest during the periods of severe exacerbation B. Regular exercise during the periods of remission C. Being in crowds during the periods of immunosuppression D. Humid environment during the periods of remission

Correct Answer: C

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Question 3 of 5

A client with HIV has been prescribed antiviral medications. What instructions related to administration of medications should the nurse give such a client?

A. Complying with the timing of antiviral medications around meals. B. Avoiding exposure to harsh sunlight for about two hours after taking the medication. C. Having the medications with plenty of fruit juice. D. Having an increased dose of the medications if the symptoms worsen.

Correct Answer: A

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Question 4 of 5

You are completing an assessment and health history for an HIV-positive patient who is considering starting antiretroviral therapy with several medications. Which patient information concerns you the most?

A. Patient has been HIV positive for 8 years and has never been on any drug therapy for the HIV infection. B. Patient tells you that he never has been very consistent about taking medications in the past. C. Patient continues to be sexually active with multiple partners and says that he is careful to use condoms. D. Patient has many questions and concerns regarding how effective and safe the medications are.

Correct Answer: B

Rationale: Adherence to antiretroviral therapy is critical for its success. If the patient has a history of inconsistent medication use this raises concerns about the effectiveness of the therapy and the risk of developing drug resistance. The other information while important does not directly impact the success of the treatment as much as adherence does.

Question 5 of 5

You have developed a nursing diagnosis of Imbalanced Nutrition: Less than Body Requirements for a hospitalized patient with AIDS who has anorexia and nausea. Which of these nursing actions is most appropriate to delegate to an LPN who is providing care to this patient?

A. Administer oxandrolone (Oxandrin) 5 mg daily in morning. B. Provide oral care with a soft toothbrush every 8 hours. C. Instruct the patient about a high-calorie,high-protein diet. D. Assess the patient for other nutrition risk factors.

Correct Answer: B

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Which of the following enzymes removes 5' phosphate groups thereby preventing or decreasing the self-sealing of restricted plasmid vector molecules?

Nurselytic

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