ATI RN
microbiology chapter 13 test bank Questions
Question 1 of 9
Which of the following does NOT refer to viruses
Correct Answer: D
Rationale: The correct answer is D because viruses are sensitive to interferon, a natural defense mechanism of the body against viral infections. Interferon helps to inhibit viral replication. A: Viruses are cell-free infectious particles, not living systems. B: Viruses are obligate intracellular parasites as they require host cells to replicate. C: Viruses can have either DNA or RNA as genetic material, which is a characteristic feature.
Question 2 of 9
A fecal sample from a patient with diarrhea revealed cysts with four nuclei. What is the most likely causative agent?
Correct Answer: A
Rationale: The correct answer is A: Entamoeba histolytica. This is because the presence of cysts with four nuclei is characteristic of Entamoeba histolytica, a protozoan parasite that causes amoebic dysentery. Entamoeba histolytica cysts contain four nuclei, distinguishing them from other pathogens. Giardia lamblia and Trichomonas vaginalis are not associated with cysts containing four nuclei. Balantidium coli is a ciliated protozoan that does not typically form cysts with multiple nuclei. In summary, the unique feature of four nuclei in the cysts points towards Entamoeba histolytica as the most likely causative agent of the diarrhea in this patient.
Question 3 of 9
A patient with a deep tissue infection had Gram-negative rods isolated from the wound culture. The bacteria were oxidase-positive and non-lactose fermenters. What is the most likely causative agent?
Correct Answer: A
Rationale: The correct answer is A: Pseudomonas aeruginosa. This is because Pseudomonas is a Gram-negative rod, oxidase-positive, and non-lactose fermenter. These characteristics are typical of Pseudomonas species. B: Escherichia coli is a Gram-negative rod but is lactose fermenter and usually oxidase-negative. C: Klebsiella pneumoniae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative. D: Shigella dysenteriae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative and is more associated with causing shigellosis rather than deep tissue infections.
Question 4 of 9
A wound culture revealed Gram-negative rods that are lactose non-fermenters. The bacteria were isolated from a patient with a deep tissue infection. What is the most likely microorganism?
Correct Answer: A
Rationale: The correct answer is A: Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative rod that is a lactose non-fermenter commonly associated with deep tissue infections. It is an opportunistic pathogen known for its ability to cause infections in immunocompromised individuals. P. aeruginosa is often resistant to multiple antibiotics and can form biofilms, making it difficult to treat. Summary of why other choices are incorrect: B: Escherichia coli is a Gram-negative rod that ferments lactose and is commonly found in the gastrointestinal tract. It is not typically associated with deep tissue infections. C: Salmonella typhi is a Gram-negative rod that causes typhoid fever, primarily affecting the gastrointestinal system, not deep tissue infections. D: Shigella dysenteriae is a Gram-negative rod that causes bacillary dysentery, which primarily affects the gastrointestinal tract and does not typically cause deep tissue infections.
Question 5 of 9
A 10 year old child was subjected to Mantoux test (with tuberculin). 48 hours later a papule up to 8 mm in diameter appeared on the site of tuberculin injection. What type of hyperesponsiveness reaction has developed after tuberculin injection?
Correct Answer: A
Rationale: The correct answer is A: Hyperresponsiveness reaction type IV. This type of reaction, also known as delayed-type hypersensitivity, is characterized by the activation of T lymphocytes and macrophages, leading to inflammation at the site of antigen exposure. In the case of the Mantoux test, the appearance of a papule 48 hours later indicates a delayed hypersensitivity reaction mediated by T cells. Choice B: Reaction of Arthus phenomenon type is incorrect as this type of reaction involves immune complexes forming in the blood vessels leading to tissue damage, which is not the mechanism seen in the Mantoux test reaction. Choice C: Reaction of serum sickness type is incorrect as this type of reaction involves the formation of immune complexes in the blood leading to systemic symptoms like fever, rash, and joint pain, which is not typically seen in the Mantoux test reaction. Choice D: Atopic reaction is incorrect as atopic reactions involve IgE antibodies and mast cell degranulation, which are not the mechanisms
Question 6 of 9
While studying the pneumonic infection in 1928 the English doctor Frederick Griffith discovered:
Correct Answer: B
Rationale: The correct answer is B: Transformation. Frederick Griffith discovered bacterial transformation, where genetic material can be transferred between bacteria. He observed this phenomenon while studying pneumonia-causing bacteria in 1928. Transformation is the process by which bacteria take up external DNA and incorporate it into their own genome. This discovery was crucial in understanding how genetic information can be transferred between organisms. Rationale for other choices: A: Translation - This term refers to the process of protein synthesis from mRNA, not relevant to Griffith's discovery. C: Transcription - This term refers to the process of synthesizing RNA from DNA, not related to Griffith's discovery. D: Conjugation - This term refers to the direct transfer of genetic material between bacterial cells through cell-to-cell contact, which is different from the phenomenon Griffith observed.
Question 7 of 9
Which of the following microorganisms can be cultured only in the footpads of mice or a species of armadillo?
Correct Answer: B
Rationale: The correct answer is B: Mycobacterium leprae. This microorganism can only be cultured in the footpads of mice or a species of armadillo due to its specific growth requirements. Mycobacterium tuberculosis (choice A) can be cultured in various culture media in the laboratory. Neisseria gonorrhoeae (choice C) can be cultured on selective media like Thayer-Martin agar. Mycoplasma (choice D) can be cultured in cell cultures or specialized media. Therefore, the unique growth pattern of Mycobacterium leprae makes it the correct answer.
Question 8 of 9
During the skill-building session in microbiology the students need to stain the prepared and fixed sputum smears obtained from a tuberculosis patient. What staining technique should be used in this case?
Correct Answer: C
Rationale: The correct staining technique for staining sputum smears from a tuberculosis patient is Ziehl-Neelsen staining. This technique uses a carbol fuchsin stain that specifically stains acid-fast organisms like Mycobacterium tuberculosis. The steps involved include staining with carbol fuchsin, decolorizing with acid-alcohol, counterstaining with methylene blue, and then examining under a microscope. This staining method is crucial for detecting the acid-fast bacilli characteristic of tuberculosis. Explanation for incorrect choices: A: Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative, not suitable for acid-fast organisms like Mycobacterium tuberculosis. B: Giemsa staining is used for detecting parasites and certain types of white blood cells, not specific for acid-fast bacteria. D: Burry staining is not a recognized staining technique in microbiology for staining acid-fast organisms.
Question 9 of 9
Sanitary important bacteria for control of air quality are:
Correct Answer: B
Rationale: The correct answer is B: Pseudomonas aeruginosa. This bacterium is important for controlling air quality as it can degrade a wide range of organic compounds present in the air, helping to maintain a clean and healthy environment. Alpha and beta-hemolytic streptococci (Choice A) are not typically associated with air quality control. E. coli (Choice C) and Salmonella spp. (Choice D) are more commonly found in water and food sources, not air, making them incorrect choices for this question. Pseudomonas aeruginosa's ability to break down organic matter in the air makes it the most suitable option for maintaining sanitary air quality.