Which of the following best describes a Gram-negative bacterium?

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Question 1 of 9

Which of the following best describes a Gram-negative bacterium?

Correct Answer: B

Rationale: The correct answer is B because Gram-negative bacteria have an outer membrane with lipopolysaccharides, which is a key characteristic used in Gram staining. This outer membrane is responsible for the pink/red color seen when stained with Gram stain. Choice A is incorrect because Gram-negative bacteria have a thin peptidoglycan layer. Choice C is incorrect because Gram-negative bacteria stain pink, not purple. Choice D is incorrect because all bacteria have a cell wall, but Gram-negative bacteria have a different structure compared to Gram-positive bacteria.

Question 2 of 9

Saprophytes are microorganisms which:

Correct Answer: B

Rationale: The correct answer is B: Cannot induce infectious disease. Saprophytes are microorganisms that obtain nutrients from decaying organic matter and do not cause infectious diseases in humans. They play a vital role in the ecosystem by decomposing dead organisms. Choice A is incorrect because saprophytes do not cause typical infections. Choice C is incorrect because saprophytes do not cause chronic infections. Choice D is incorrect because saprophytes do not cause mixed infections involving multiple pathogens. Therefore, the correct answer is B as saprophytes do not induce infectious diseases.

Question 3 of 9

A patient with acute food poisoning had fecal cultures grown on Endo agar, resulting in colorless colonies. What microorganism is most likely responsible?

Correct Answer: A

Rationale: Step 1: Endo agar is a selective and differential media commonly used to isolate gram-negative bacteria. Step 2: Colorless colonies on Endo agar indicate the absence of lactose fermentation. Step 3: Salmonella is a gram-negative bacterium that does not ferment lactose, leading to colorless colonies on Endo agar. Step 4: Therefore, the correct answer is A: Salmonella. Summary: B: Escherichia coli ferments lactose, producing pink colonies on Endo agar. C: Staphylococcus is a gram-positive bacterium and would not grow on Endo agar. D: Shigella is a gram-negative bacterium but ferments lactose, resulting in pink colonies on Endo agar.

Question 4 of 9

Bacteria that use oxygen, but only at low concentration, are classified as:

Correct Answer: B

Rationale: The correct answer is B: microaerophiles. These bacteria require oxygen to grow, but only at low concentrations as higher levels can be toxic. They possess enzymes to deal with reactive oxygen species. Obligate aerobes (A) require oxygen for growth, obligate anaerobes (C) cannot survive in the presence of oxygen, and aerotolerant anaerobes (D) can tolerate oxygen but do not use it for growth.

Question 5 of 9

Which of the following best describes a Gram-negative bacterium?

Correct Answer: B

Rationale: The correct answer is B because Gram-negative bacteria have an outer membrane with lipopolysaccharides, which is a key characteristic used in Gram staining. This outer membrane is responsible for the pink/red color seen when stained with Gram stain. Choice A is incorrect because Gram-negative bacteria have a thin peptidoglycan layer. Choice C is incorrect because Gram-negative bacteria stain pink, not purple. Choice D is incorrect because all bacteria have a cell wall, but Gram-negative bacteria have a different structure compared to Gram-positive bacteria.

Question 6 of 9

Which of the following viruses belong to family Arenaviridae

Correct Answer: A

Rationale: The correct answer is A: Lassa virus. The rationale is as follows: 1. Lassa virus is a member of the Arenaviridae family, known for causing Lassa fever. 2. Arenaviridae family includes viruses with a segmented genome and are transmitted through rodents. 3. Crimean-Congo hemorrhagic fever virus (Choice B) belongs to the Bunyaviridae family, not Arenaviridae. 4. Rabies virus (Choice C) belongs to the Rhabdoviridae family, not Arenaviridae. 5. Epstein-Barr virus (Choice D) belongs to the Herpesviridae family, not Arenaviridae. In summary, Lassa virus is the correct choice as it aligns with the characteristics of the Arenaviridae family, while the other choices belong to different virus families.

Question 7 of 9

Microscopic examination of a smear from a patient with gas gangrene revealed Gram-positive rods producing spores. What microorganism was identified?

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. Gas gangrene is caused by Clostridium perfringens, a Gram-positive rod bacterium that produces spores. This bacterium is anaerobic and commonly found in soil, where it forms spores that can survive harsh conditions. Clostridium tetani causes tetanus, not gas gangrene. Bacillus anthracis causes anthrax, not gas gangrene. Clostridium botulinum causes botulism, not gas gangrene. Therefore, based on the characteristics of the microorganism described in the question, Clostridium perfringens is the correct identification.

Question 8 of 9

Detection of galactomannan and free mannan is used for diagnosing:

Correct Answer: A

Rationale: The correct answer is A: Aspergillosis and candidiasis. Galactomannan is a polysaccharide found in Aspergillus cell walls and is detected in patients with invasive aspergillosis. Free mannan is a polysaccharide found in Candida cell walls and is detected in patients with candidiasis. Therefore, detection of galactomannan and free mannan is specific for diagnosing Aspergillosis and candidiasis. Choices B, C, and D are incorrect as HIV infection, syphilis, and leptospirosis do not involve the detection of galactomannan or free mannan for diagnosis.

Question 9 of 9

Tinea capitis is a fungal infection of the:

Correct Answer: B

Rationale: The correct answer is B: scalp. Tinea capitis is a fungal infection that specifically affects the scalp. This infection is caused by dermatophytes, which thrive in warm and moist environments. It typically presents with symptoms such as scaling, itching, and hair loss on the scalp. Choices A, C, and D are incorrect because tinea pedis (athlete's foot) affects the feet, oral thrush is a fungal infection of the mouth, and tinea manuum affects the hands. By process of elimination and understanding the specific characteristics of tinea capitis, we can determine that the scalp is the correct location for this fungal infection.

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