Which of the following bacteria should be handled in a Biosafety Level 2 facility?

Correct Answer: C

Rationale: The correct answer is C: Salmonella. This bacterium should be handled in a Biosafety Level 2 facility due to its potential to cause disease in humans. Salmonella is a pathogenic bacterium that can cause foodborne illnesses and infections. Handling it in a Biosafety Level 2 facility ensures proper containment and protection of personnel. A: Micrococcus luteus and B: Bacillus subtilis are common environmental bacteria that do not pose significant risks to human health, so they can be safely handled in lower biosafety levels. D: Staphylococcus epidermidis is a common skin commensal bacterium that is generally not considered highly pathogenic, so it does not require Biosafety Level 2 containment.

Correct Answer: A

Rationale: The correct answer is A: Neisser staining method. Neisser staining method is used to stain diphtheria bacilli, which appear as blue rods with thickenings at the poles. The rationale is that Neisser staining specifically targets and highlights the characteristics of the diphtheria bacilli. Incorrect choices: B: Gram staining is not specific for diphtheria bacilli and does not show the characteristic blue rods with thickenings at the poles. C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for diphtheria bacilli. D: Loeffler staining is used for diphtheria bacilli but does not produce blue rods with thickenings at the poles as seen in this case.

Correct Answer: A

Rationale: The correct answer is A: Toxoplasmosis. Toxoplasmosis is a parasitic infection caused by Toxoplasma gondii, which can be transmitted from mother to fetus during pregnancy, leading to congenital toxoplasmosis. This infection can result in stillbirth, multiple developmental defects, and intrauterine death. Leishmaniasis (B), Malaria (C), and Amebiasis (D) are caused by different parasites and do not typically lead to intrauterine death or developmental defects in the fetus.

Correct Answer: A

Rationale: The correct answer is A: germination. In the lytic life cycle of phages, germination is not a stage. The lytic cycle involves adsorption, penetration, replication, assembly, and lysis. Germination is typically associated with the lysogenic cycle, where the phage DNA integrates into the host genome. Therefore, A is the correct answer. Choices B, C, and D are incorrect because they are all stages of the lytic cycle. Penetration refers to the injection of phage DNA into the host cell, adsorption is the attachment of the phage to the host cell, and lysis is the final stage where the host cell bursts, releasing new phages.

Correct Answer: B

Rationale: The correct answer is B: Vibrio cholerae. Cholera is caused by this specific bacterium due to its production of cholera toxin, leading to severe watery diarrhea. Shigella dysenteriae causes dysentery, Salmonella enterica causes salmonellosis, and Clostridium perfringens causes food poisoning, none of which present with the characteristic symptoms of cholera.