Which of the following bacteria should be handled in a Biosafety Level 2 facility?

Correct Answer: C

Rationale: The correct answer is C: Salmonella. This bacterium should be handled in a Biosafety Level 2 facility due to its potential to cause disease in humans. Salmonella is a pathogenic bacterium that can cause foodborne illnesses and infections. Handling it in a Biosafety Level 2 facility ensures proper containment and protection of personnel. A: Micrococcus luteus and B: Bacillus subtilis are common environmental bacteria that do not pose significant risks to human health, so they can be safely handled in lower biosafety levels. D: Staphylococcus epidermidis is a common skin commensal bacterium that is generally not considered highly pathogenic, so it does not require Biosafety Level 2 containment.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Adenoviruses are DNA viruses, so choices B and C (ssRNA and DNA) are incorrect. 2. Adenoviruses have a middle-sized, naked icosahedral capsid, making choice A the correct description. 3. Choice D is incorrect as adenoviruses do have specific characteristics, making "None of the above" an inaccurate option. In summary, the correct answer A is supported by the fact that adenoviruses have a middle-sized, naked icosahedral capsid and contain double-stranded DNA. Choices B, C, and D are incorrect as they do not align with the characteristics of adenoviruses.

Correct Answer: A

Rationale: The correct answer is A: Cell wall. Mycoplasma species lack a cell wall, making them unique among bacteria. This is because they have a flexible cell membrane that provides structural support instead of a rigid cell wall. The absence of a cell wall allows them to be pleomorphic and gives them resistance to certain antibiotics targeting cell wall synthesis. Plasma membrane (B), DNA (C), and ribosomes (D) are present in Mycoplasma species, so they are not the correct answer.

Correct Answer: A

Rationale: The correct answer is A: Hyperresponsiveness reaction type IV. This type of reaction, also known as delayed-type hypersensitivity, is characterized by the activation of T lymphocytes and macrophages, leading to inflammation at the site of antigen exposure. In the case of the Mantoux test, the appearance of a papule 48 hours later indicates a delayed hypersensitivity reaction mediated by T cells. Choice B: Reaction of Arthus phenomenon type is incorrect as this type of reaction involves immune complexes forming in the blood vessels leading to tissue damage, which is not the mechanism seen in the Mantoux test reaction. Choice C: Reaction of serum sickness type is incorrect as this type of reaction involves the formation of immune complexes in the blood leading to systemic symptoms like fever, rash, and joint pain, which is not typically seen in the Mantoux test reaction. Choice D: Atopic reaction is incorrect as atopic reactions involve IgE antibodies and mast cell degranulation, which are not the mechanisms

Correct Answer: B

Rationale: The correct answer is B because sterilization is the process of completely destroying all microorganisms, including resistant forms like spores, ensuring no viable organisms remain. This is crucial for ensuring complete elimination of potential pathogens. Choice A is incorrect because sterilization should eliminate all microorganisms, including spores. Choice C is incorrect as it describes disinfection, not sterilization. Choice D is incorrect because sterilization aims to eradicate all microorganisms, not leave any viable ones behind.

Correct Answer: A

Rationale: The most probable diagnosis is A: Candidous cheilitis. Candidous cheilitis is a fungal infection caused by Candida species. In this case, the presence of mycelium on the plaques indicates a fungal infection. The symptoms of pain, burning, and grey plaques that can be easily removed leaving hyperemia are typical for Candidous cheilitis. Choice B (Exfoliative cheilitis) is characterized by scaling and peeling of the lips, not plaques with mycelium. Choice C (Leukoplakia) is a precancerous condition causing white plaques that cannot be easily removed. Choice D (Contactant allergic cheilitis) is an allergic reaction causing inflammation, not fungal plaques.

Correct Answer: A

Rationale: The correct answer is A: yeast and molds. Yeast and molds can resist acidic pH due to their ability to maintain a stable internal pH through various mechanisms such as ion transport and cell wall composition. Bacteria, including E. coli, are generally more sensitive to acidic conditions. Choice D is incorrect as yeast and molds are known to thrive in acidic environments.

Correct Answer: B

Rationale: The correct answer is B: eukaryotic. Eukaryotic cells have a well-defined nucleus that encloses the genetic material. The nucleus is a distinguishing feature of eukaryotic cells, separating them from prokaryotic cells (choice A) which lack a nucleus. Anucleate cells (choice C) are cells that do not have a nucleus at all. Haploid cells (choice D) contain a single set of chromosomes, but this does not necessarily imply the presence of a nucleus. Therefore, the presence of a nucleus is what categorizes cells as eukaryotic.

Correct Answer: A

Rationale: The correct answer is A: Neisser staining method. Neisser staining method is used to stain diphtheria bacilli, which appear as blue rods with thickenings at the poles. The rationale is that Neisser staining specifically targets and highlights the characteristics of the diphtheria bacilli. Incorrect choices: B: Gram staining is not specific for diphtheria bacilli and does not show the characteristic blue rods with thickenings at the poles. C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for diphtheria bacilli. D: Loeffler staining is used for diphtheria bacilli but does not produce blue rods with thickenings at the poles as seen in this case.