Which of the following bacteria produce endotoxins?

Correct Answer: C

Rationale: The correct answer is C: Escherichia coli. E. coli is a gram-negative bacterium, which means it has an outer membrane containing lipopolysaccharides (LPS), also known as endotoxins. Endotoxins are released when the bacterium is destroyed, causing an inflammatory response. Streptococcus pneumoniae (choice A) is a gram-positive bacterium and does not produce endotoxins. Clostridium tetani (choice B) produces exotoxins, not endotoxins. Staphylococcus aureus (choice D) is also a gram-positive bacterium and does not produce endotoxins. In summary, E. coli is the correct answer because it is a gram-negative bacterium that produces endotoxins, while the other choices are gram-positive bacteria that do not produce endotoxins.

Correct Answer: D

Rationale: The correct answer is D: BCG vaccination. BCG vaccination is recommended for individuals with a negative Mantoux test result, especially in countries where tuberculosis is prevalent. This vaccination can help provide protection against tuberculosis. Choice A is incorrect because repeating the Mantoux test in a month is unnecessary since the boy already had a negative reaction. Choice B is incorrect as serodiagnosis of tuberculosis is not the first-line approach for diagnosis, especially in this scenario. Choice C is incorrect because urgent isolation is not necessary based solely on the Mantoux test result. Isolation is typically reserved for individuals with active tuberculosis.

Correct Answer: A

Rationale: The correct answer is A: cultivating on artificial media and biochemical identification. This method is appropriate for diagnosing listeriosis because it allows for the isolation and growth of Listeria species on selective media, followed by biochemical tests to confirm the identity. Serological diagnosis with ELISA (choice B) is not as specific for Listeria and may yield false positives. Choice C, serological diagnosis with agglutination hype Widal, is not typically used for listeriosis diagnosis. Cultivating in cell cultures (choice D) is not the primary method for diagnosing listeriosis as it is less specific compared to cultivating on artificial media and biochemical identification.

Correct Answer: D

Rationale: The correct answer is D: Fibers at the vertices of the icosahedron. Adenoviruses have fibers at the vertices of the icosahedron, which give them their distinctive pattern. These fibers help in attaching to host cells. Choices A, B, and C are incorrect because adenoviruses do not have a lipid envelope, helical capsid, or ssDNA. Adenoviruses have a non-enveloped icosahedral capsid and contain dsDNA. This makes choice D the correct answer as it specifically relates to the unique structural feature of adenoviruses.

Correct Answer: B

Rationale: The correct answer is B: Gram negative coccobacillus. Bordetella pertussis, the causative agent of pertussis, is a gram-negative coccobacillus. This is because it is a small, rod-shaped bacterium that appears both as a bacillus (rod-shaped) and a coccus (spherical) shape. It does not retain the crystal violet stain in the Gram staining process, indicating its negative gram nature. Choice A is incorrect because pertussis is not a gram-positive bacillus. Choice C is incorrect because pertussis is not a long gram-negative rod-shaped bacterium. Choice D is incorrect as the other options do not accurately describe the morphology of Bordetella pertussis.

Correct Answer: B

Rationale: The correct answer is B: Mycelium. Mycelium refers to the network of branching filaments that form the main body of a fungus. When fungi grow on solid media, they develop a visible mass of mycelium. Hyphae (A) are the individual filaments that make up mycelium. Spores (C) are reproductive cells produced by fungi. Columella (D) refers to a specialized structure found in some fungi but is not related to the colonies grown on solid media. Therefore, the correct term for the colonies of fungi on solid media is mycelium.

Correct Answer: C

Rationale: Rationale: Choice C is correct because Staphylococcus epidermidis, Escherichia coli, and Enterococcus spp. are common causative agents of nosocomial infections due to their presence on the skin, in the gut, and in healthcare settings. S. aureus and L. monocytogenes (Choice A) are also important pathogens but are less common in nosocomial infections. Enterobacteriaceae (Choice B) are a large family of bacteria that can cause infections but are not as commonly associated with nosocomial infections compared to the organisms in Choice C. The correct answer (Choice C) encompasses pathogens commonly found in healthcare settings, making it the most suitable option.

Correct Answer: B

Rationale: Certainly! The correct answer is B: Facultative anaerobes. Facultative anaerobes can survive in both aerobic and anaerobic environments due to their ability to switch between utilizing oxygen for respiration in aerobic conditions and utilizing fermentation or anaerobic respiration in anaerobic conditions. This flexibility allows them to thrive in diverse environments. Explanation for incorrect choices: A: Obligate aerobes require oxygen for respiration and cannot survive in anaerobic environments. C: Obligate anaerobes can only survive in the absence of oxygen and would not thrive in aerobic conditions. D: Microaerophiles require low levels of oxygen to grow and are not as versatile in adapting to varying oxygen levels compared to facultative anaerobes.

Correct Answer: A

Rationale: The correct answer is A: Bacterial DNA. PCR analysis detects specific DNA sequences, so a positive result for H. pylori indicates the presence of the bacterial DNA in the gastric biopsy specimen. This method identifies the genetic material of the bacterium, not the bacterium itself (choice C) or its toxins (choice B). Additionally, PCR does not directly detect bacterial enzymes (choice D) but rather focuses on genetic material for accurate identification. Therefore, in this case, the detection of H. pylori in the PCR analysis points to the presence of bacterial DNA, confirming the etiology of peptic ulcer disease.