Which of the following bacteria are capable of producing an exotoxin that causes severe diarrhea?

Correct Answer: D

Rationale: The correct answer is D because all three bacteria mentioned (Clostridium botulinum, Vibrio cholerae, and Escherichia coli) are capable of producing exotoxins that cause severe diarrhea. Clostridium botulinum produces botulinum toxin leading to botulism, Vibrio cholerae produces cholera toxin causing cholera, and some strains of Escherichia coli produce enterotoxins causing severe diarrhea. Choices A, B, and C alone are incorrect because each of them can produce exotoxins that cause severe diarrhea.

Correct Answer: D

Rationale: The correct answer is D: Positive strand RNA viruses. These viruses have RNA genomes that can be directly translated by host ribosomes without the need for transcription. In contrast, single-strand DNA and double-strand DNA viruses require transcription to generate mRNA for translation. Choice A is incorrect as there is a correct answer. Choice B and C are incorrect because both single-strand DNA and double-strand DNA viruses require transcription for mRNA synthesis.

Correct Answer: D

Rationale: The correct answer is D: protozoan. Babesiosis is caused by a protozoan parasite called Babesia. Protozoans are single-celled organisms that can cause various diseases in humans. Babesiosis is transmitted to humans through the bite of infected ticks. Bacteria (choice A), viruses (choice B), and helminths (choice C) are not responsible for causing babesiosis. Bacteria are typically responsible for diseases like Lyme disease, viruses for illnesses like Zika, and helminths for infections such as parasitic worms. Thus, the correct choice for the causative agent of babesiosis is a protozoan.

Correct Answer: B

Rationale: The correct answer is B: glycocalyx. The glycocalyx is a matrix formed outside the plasma membrane that consists of glycoproteins and glycolipids. It plays crucial roles in cell-cell recognition, protection, and immune response. Cytoplasm (A) is the gel-like substance inside the cell, not outside. Exoplasm (C) is not a recognized term in biology. Cell wall (D) is a rigid structure found in plant cells, not outside the plasma membrane in all cells.

Correct Answer: D

Rationale: The correct answer is D: Fibers at the vertices of the icosahedron. Adenoviruses have fibers at the vertices of the icosahedron, which give them their distinctive pattern. These fibers help in attaching to host cells. Choices A, B, and C are incorrect because adenoviruses do not have a lipid envelope, helical capsid, or ssDNA. Adenoviruses have a non-enveloped icosahedral capsid and contain dsDNA. This makes choice D the correct answer as it specifically relates to the unique structural feature of adenoviruses.

Correct Answer: A

Rationale: The correct answer is A: Bacterial DNA. PCR analysis detects specific DNA sequences, so a positive result for H. pylori indicates the presence of the bacterial DNA in the gastric biopsy specimen. This method identifies the genetic material of the bacterium, not the bacterium itself (choice C) or its toxins (choice B). Additionally, PCR does not directly detect bacterial enzymes (choice D) but rather focuses on genetic material for accurate identification. Therefore, in this case, the detection of H. pylori in the PCR analysis points to the presence of bacterial DNA, confirming the etiology of peptic ulcer disease.

Correct Answer: A

Rationale: The correct answer is A: Neisser staining. This method is specifically used to identify cytoplasmic inclusions by staining them with a specific dye that highlights these structures. Neisser staining is designed to selectively target and visualize cytoplasmic inclusions, making it the most appropriate choice for this purpose. Option B: Clett staining, Option C: Ziehl-Nielsen staining, and Option D: Gram staining are not typically used for identifying cytoplasmic inclusions. Clett staining is not a commonly known staining method for this purpose. Ziehl-Nielsen staining is primarily used to detect acid-fast bacteria like Mycobacterium tuberculosis. Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative based on cell wall characteristics. Therefore, these options are not suitable for identifying cytoplasmic inclusions.

Correct Answer: C

Rationale: Rationale: Choice C is correct because Staphylococcus epidermidis, Escherichia coli, and Enterococcus spp. are common causative agents of nosocomial infections due to their presence on the skin, in the gut, and in healthcare settings. S. aureus and L. monocytogenes (Choice A) are also important pathogens but are less common in nosocomial infections. Enterobacteriaceae (Choice B) are a large family of bacteria that can cause infections but are not as commonly associated with nosocomial infections compared to the organisms in Choice C. The correct answer (Choice C) encompasses pathogens commonly found in healthcare settings, making it the most suitable option.

Correct Answer: B

Rationale: The correct answer is B: Personal eyewash. Personal eyewash is designed for immediate flushing until the victim reaches a more suitable safety unit. It provides a controlled flow of water to safely flush eyes while transporting the victim. The emergency shower (A) is meant for full-body coverage, not just for the eyes. Handheld drench hose (C) may not provide adequate flushing pressure. Combination unit (D) may not be easily portable for immediate use.