Which bacteria are associated with the disease tuberculosis?

Correct Answer: B

Rationale: The correct answer is B: Mycobacterium tuberculosis. This bacterium is specifically associated with causing tuberculosis. Mycobacterium leprae (choice A) is associated with leprosy, not tuberculosis. Streptococcus pneumoniae (choice C) is associated with pneumonia, not tuberculosis. Escherichia coli (choice D) is a common bacterium found in the intestines and is not associated with tuberculosis. Mycobacterium tuberculosis is the only bacterium directly linked to the disease, making it the correct choice.

Correct Answer: B

Rationale: Decontamination is the reduction or removal of unwanted chemical or biological agents to make an area safe. This includes cleaning, disinfecting, or sterilizing to eliminate harmful substances. Choice A is incorrect as decontamination doesn't necessarily involve killing all microorganisms. Choice C is incorrect as it focuses on stopping growth, not removing existing contaminants. Choice D is incorrect as it specifically mentions vegetative organisms, which may not cover all types of contaminants that need to be removed during decontamination. Thus, the correct answer is B as it accurately describes the process of decontamination.

Correct Answer: D

Rationale: Conjugation involves direct cell-to-cell contact for the transfer of genetic material, typically through a pilus. This process is well-documented in bacterial genetics. Transformation (A) involves uptake of free DNA from the environment. Transduction (B) is genetic transfer by a viral vector. Replication (C) is the process of producing identical copies of DNA or RNA. Therefore, the correct answer is D (conjugation) as it specifically refers to the one-way transfer of genetic material through direct cell-to-cell contact.

Correct Answer: A

Rationale: The correct answer is A: Neutralization. In this experiment, the researchers used antitoxic antibotulinic serum of different types to neutralize the botulinic toxin in tinned meat extract injected into mice. Neutralization is the process of rendering a toxin harmless by combining it with specific antibodies. The purpose of injecting the antitoxic serum was to neutralize the toxin and prevent it from causing harm to the mice. This serological reaction of neutralization directly aligns with the objective of the experiment. Summary of other choices: B: Precipitation - Precipitation involves the formation of a visible insoluble complex when an antigen reacts with its corresponding antibody. This process was not described in the scenario. C: Complement binding - Complement binding involves the activation of the complement system by antigen-antibody complexes. This was not the main focus of the experiment described. D: Double immune diffusion - This technique is used to detect the presence of specific antigens or antibodies

Correct Answer: A

Rationale: The correct answer is A: Β-lactamase. In ELISA, enzymes like alkaline phosphatase, horseradish peroxidase, and Β-galactosidase are commonly used as labels to detect the presence of specific molecules. Β-lactamase is not typically used in ELISA as it is an enzyme used to hydrolyze β-lactam antibiotics. Therefore, it does not serve the purpose of detecting specific molecules in ELISA. Alkaline phosphatase, horseradish peroxidase, and Β-galactosidase are preferred enzymes in ELISA due to their ability to produce a detectable signal when bound to specific molecules.

Correct Answer: B

Rationale: The correct answer is B: Streptolysin. Streptolysin is a toxin released by Streptococcus pyogenes that causes lysis of red blood cells and contributes to the pathogenicity of the bacteria. Enterotoxin (Choice A) is typically produced by Staphylococcus aureus, not Streptococcus pyogenes. Staphylocoagulase (Choice C) is an enzyme produced by Staphylococcus aureus to promote blood clot formation and is not associated with Streptococcus pyogenes. Therefore, the correct answer is B as Streptolysin is the toxin released by Streptococcus pyogenes.

Correct Answer: D

Rationale: The correct answer is D: All of the above. Hospital-acquired infections can be caused by a variety of bacteria commonly found in healthcare settings. Staphylococcus aureus is a common cause of surgical site infections. Escherichia coli can cause urinary tract infections. Klebsiella pneumoniae is associated with pneumonia and bloodstream infections. Therefore, all three bacteria are frequently implicated in hospital-acquired infections due to their prevalence and ability to cause different types of infections in healthcare settings. Thus, the correct answer is D, as all of the listed bacteria are commonly associated with hospital-acquired infections.

Correct Answer: C

Rationale: The correct answer is C: Rickettsia. Rickettsia is a host-dependent bacterium because it requires living cells of a host organism to survive and replicate. This bacterium is an obligate intracellular pathogen, meaning it cannot grow outside of a host cell. A: Mycoplasma and B: Ureaplasma are not host-dependent as they can grow and reproduce in cell-free environments due to possessing their own unique cell wall structures. D: Micrococcus is a free-living bacterium that can survive and reproduce independently without relying on a host organism.

Correct Answer: A

Rationale: The correct answer is A: Delayed-type hypersensitivity. The Mantoux test is a type IV hypersensitivity reaction, which is also known as delayed-type hypersensitivity. This reaction typically occurs 48-72 hours after exposure to an antigen, such as tuberculin in this case. The focal skin hardening and redness that appeared 60 hours after tuberculin introduction align with the timeline and characteristics of a delayed-type hypersensitivity reaction. Summary: - Choice A (Delayed-type hypersensitivity): Correct, as explained above. - Choice B (Immune complex-mediated hypersensitivity): Incorrect, as this reaction involves immune complexes and typically presents with tissue damage and inflammation. - Choice C (Complement-mediated cytotoxic hypersensitivity): Incorrect, as this reaction involves the activation of complement proteins leading to cell lysis. - Choice D (Immediate hypersensitivity): Incorrect, as this reaction, such as in allergies, occurs within minutes to hours of exposure to an antigen and involves IgE-mediated mast