ATI RN
microbiology an introduction tortora test bank Questions
Question 1 of 5
Which are the most suitable materials for isolation of polio viruses
Correct Answer: D
Rationale: The correct answer is D because polio viruses are primarily found in the gastrointestinal tract and respiratory secretions. Feces, nasopharyngeal swabs, and cerebrospinal fluid are the most suitable materials for isolating polio viruses due to their presence in these body fluids. Feces contain the virus shed from the intestines, nasopharyngeal swabs collect respiratory secretions where the virus may be present, and cerebrospinal fluid is important for detecting the virus in cases of neurological complications. Choices A and B include irrelevant body fluids that are not typically associated with polio virus transmission. Option C is too limited as the virus can also be present in respiratory secretions and cerebrospinal fluid, not just feces.
Question 2 of 5
During the skill-building session in microbiology the students need to stain the prepared and fixed sputum smears obtained from a tuberculosis patient. What staining technique should be used in this case?
Correct Answer: C
Rationale: The correct staining technique for sputum smears obtained from a tuberculosis patient is Ziehl-Neelsen staining. This technique uses carbol fuchsin dye to stain the acid-fast bacteria, such as Mycobacterium tuberculosis, red. Here's a step-by-step rationale: 1. Ziehl-Neelsen staining is specifically designed to detect acid-fast bacteria like Mycobacterium tuberculosis. 2. Carbol fuchsin dye is used to penetrate the mycolic acid layer in the cell wall of the bacteria, making them resistant to decolorization. 3. Acid-alcohol is used as a decolorizer to remove the stain from non-acid-fast bacteria. 4. Methylene blue is used as a counterstain to visualize non-acid-fast bacteria in blue. Therefore, Ziehl-Neelsen staining is the most appropriate technique for identifying Mycobacterium tuberculosis in sputum smears. Other choices like Gram, Giemsa, and
Question 3 of 5
The main function of siderophores in bacteria is:
Correct Answer: B
Rationale: The correct answer is B: to sequester iron from the environment. Siderophores are molecules produced by bacteria to chelate and acquire iron, an essential nutrient for their growth and survival. By sequestering iron, bacteria can compete with host cells and other microbes for this vital resource. This enhances their ability to thrive and establish infections. Choices A, C, and D are incorrect because siderophores do not directly enhance antibiotic resistance, form capsules, or perform oxidative phosphorylation. These functions are unrelated to the primary role of siderophores in iron acquisition.
Question 4 of 5
Which of the following bacteria is responsible for causing the disease known as whooping cough?
Correct Answer: A
Rationale: Step 1: Bordetella pertussis is the causative agent of whooping cough, also known as pertussis. Step 2: This bacterium produces toxins that damage the respiratory tract, leading to severe coughing fits. Step 3: Clostridium tetani causes tetanus, Haemophilus influenzae causes respiratory infections, and Staphylococcus aureus causes skin infections. Step 4: Therefore, A is the correct answer as it is the only bacterium directly associated with whooping cough.
Question 5 of 5
Which of the following characteristics does not refer to the flu vaccine
Correct Answer: D
Rationale: The correct answer is D because the flu vaccine primarily stimulates systemic immunity rather than local immunity. It is administered parenterally (C) as it is injected into the body. It is a subunit vaccine (B) as it contains only specific antigens of the virus. It is a recommended vaccine (A) due to its effectiveness in preventing influenza. In summary, D is the correct choice as the flu vaccine mainly targets systemic immunity, not local immunity, unlike the other characteristics listed.