What outcome would you least expect if the amount of template in a PCR fell significantly below the optimal amount?

Questions 81

ATI RN

ATI RN Test Bank

Immune System Quiz Quizlet Questions

Question 1 of 5

What outcome would you least expect if the amount of template in a PCR fell significantly below the optimal amount?

Correct Answer: B

Rationale: The correct answer is B: increased yield. If the amount of template in a PCR falls significantly below the optimal amount, it would be expected to result in a decreased yield rather than an increased yield. The template is essential for DNA amplification in PCR, and a lower amount would lead to fewer copies being produced. Non-specific amplification (choice A) can occur if there is too much template or incorrect primer annealing. Allelic drop-out (choice C) is likely to happen if there is insufficient template present to amplify all alleles. Longer targets amplifying poorly or failing (choice D) is also a common outcome when template concentration is low. Therefore, the least expected outcome would be an increased yield as that goes against the principle of PCR amplification.

Question 2 of 5

Antihistamines are used cautiously in older men with prostatic hypertrophy for which of the following reasons?

Correct Answer: B

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Question 3 of 5

A client with AIDS is terminally ill and wants to name a person as a beneficiary to his life insurance in exchange for immediate cash. What is the term that best describes this arrangement?

Correct Answer: C

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Question 4 of 5

A patient who has received a kidney transplant has been admitted to the medical unit with acute rejection and is receiving IV cyclosporine (Sandimmune) and methylprednisolone (Solu-Medrol). Which staff member is best to assign to care for this patient?

Correct Answer: C

Rationale: An experienced RN who is familiar with the medical unit and its protocols is the best choice for caring for a patient with acute rejection. This patient requires close monitoring and skilled care which an experienced RN can provide. The other options involve RNs who may not be as familiar with the unit or are less experienced.

Question 5 of 5

A patient with systemic lupus erythematosus (SLE) is admitted to the hospital for evaluation and management of acute joint inflammation. Which information obtained in the admission laboratory testing concerns you most?

Correct Answer: A

Rationale: An elevated BUN level may indicate kidney involvement which is a serious complication of SLE. The other findings are expected in SLE and do not indicate an urgent problem. CRP is a marker of inflammation and positive ANA and LE cell prep are diagnostic of SLE but do not indicate acute complications.

Access More Questions!

ATI RN Basic


$89/ 30 days

ATI RN Premium


$150/ 90 days

Similar Questions