What is the correct order of steps needed for 2nd generation sequencing?

Correct Answer: C

Rationale: The correct order of steps for 2nd generation sequencing is C: DNA isolation, DNA fragmentation, primer ligation, amplification, sequencing. Firstly, DNA isolation is crucial to extract the DNA from the sample. Next, DNA fragmentation breaks the DNA into smaller fragments for sequencing. Primer ligation involves attaching primers to the DNA fragments. Amplification is necessary to make multiple copies of the DNA fragments for sequencing. Finally, sequencing is the process of determining the nucleotide sequence of the DNA fragments. This order ensures that the DNA is properly prepared and amplified before sequencing, leading to accurate results. Choices A, B, and D are incorrect because they do not follow the correct sequence of steps required for 2nd generation sequencing. Incorrect sequences may lead to errors in the sequencing process and inaccurate results.

Correct Answer: B

Rationale: The correct answer is B: Palindromic DNA. Palindromic DNA refers to a segment of DNA that reads the same from both directions. This property occurs when the nucleotide sequence on one strand of DNA is the reverse complement of the nucleotide sequence on the other strand. This symmetry allows the DNA sequence to be read the same forward and backward. Incorrect choices: A: Complementary DNA - Complementary DNA refers to a synthesized DNA strand that is complementary to a given DNA sequence. C: Copy DNA - Copy DNA is not a commonly used term in molecular biology. D: Transcribed DNA - Transcribed DNA refers to DNA that has been converted into RNA through the process of transcription, not DNA that reads the same forward and backward.

Correct Answer: D

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Correct Answer: A

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Correct Answer: D

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.