Urinalysis of a patient with acute cystitis shows leukocytes and a large number of gram-negative bacilli. Inoculation has resulted in the growth of mucous colonies that produce a green soluble pigment. What microorganism is the most likely cause of the patient's disorder?

Correct Answer: C

Rationale: The correct answer is C: Escherichia coli. E. coli is the most common cause of acute cystitis. It typically presents with leukocytes and gram-negative bacilli in the urine. The green pigment production indicates pyocyanin, which is a characteristic of E. coli. Pseudomonas aeruginosa (A) can also produce green pigment, but it is less common in urinary tract infections. Proteus mirabilis (B) is associated with a swarming motility pattern on agar, not mucous colonies. Klebsiella pneumoniae (D) is associated with pneumonia and other respiratory infections, not cystitis.

Correct Answer: B

Rationale: The correct answer is B: Leptospirosis. Spiral-shaped bacteria with loose coils and pointed ends are characteristic of Leptospira interrogans, the causative agent of Leptospirosis. This bacterium is typically found in contaminated water or soil and can infect humans through contact with infected animals or water. Syphilis (Choice A) is caused by the spirochete Treponema pallidum, which has a more tightly coiled shape and does not typically appear loose or pointed. Relapsing fever (Choice C) is caused by Borrelia species, which are also spirochetes but do not have the loose coils and pointed ends described. Lyme disease (Choice D) is caused by Borrelia burgdorferi, which, like Relapsing fever, does not match the morphology described.

Correct Answer: A

Rationale: The correct answer is A: ocular lens. The ocular lens is the lens closest to the eye in a compound light microscope. This lens is responsible for magnifying the image produced by the objective lens. It typically has a magnification power of 10x. The objective lens, choice B, is positioned closer to the specimen and provides the primary magnification of the image. The condenser, choice C, is located beneath the stage and helps focus light onto the specimen. The stage, choice D, is where the specimen is placed for observation. Therefore, the ocular lens is the correct choice as it is the lens closest to the eye and responsible for further magnifying the image.

Correct Answer: C

Rationale: The correct answer is C because Gram staining after culture on Loeffler's medium or tellurite-containing medium will show the characteristic Chinese letter arrangement of Corynebacterium diphtheriae. This arrangement consists of metachromatic granules that stain more deeply than the rest of the cell. This specific staining pattern is a key diagnostic feature of C. diphtheriae in microbiological diagnosis of diphtheria. Choice A is incorrect because simply identifying the isolate as C. diphtheriae is not sufficient for diagnosis. Choice B is incorrect as C. diphtheriae can be stained via Gram staining despite its small cell size. Choice D is incorrect as option C is indeed correct for microbiological diagnosis of diphtheria.

Correct Answer: C

Rationale: The correct answer is C: Ebola virus. The patient's symptoms align with Ebola virus infection, including fever, severe hemorrhagic syndrome, and CNS involvement. Ebola virus is known to cause hemorrhagic fevers with high mortality rates. A: SARS CoV causes Severe Acute Respiratory Syndrome, which typically presents with respiratory symptoms, not hemorrhagic fever. B: Lymphocytic choriomeningitis virus usually causes a mild illness with flu-like symptoms, not severe hemorrhagic syndrome. D: ECHO 9 virus is associated with aseptic meningitis, not hemorrhagic fever.

Correct Answer: A

Rationale: The correct answer is A because significant bacteriuria is defined as having more than 10^5 cells in 1 ml of midstream morning urine. This concentration indicates a true infection rather than contamination. Choice B is incorrect because the amount of urine does not determine significant bacteriuria. Choice C is incorrect because even a single bacterium in urine does not necessarily indicate an infection. Choice D is incorrect because not all statements are correct; only choice A accurately defines significant bacteriuria based on the bacterial concentration in urine samples.

Correct Answer: D

Rationale: The correct answer is D: Agar media. Influenza viruses cannot be isolated in agar media because they require specific conditions present in laboratory animals, embryonated eggs, or cell cultures for successful isolation and propagation. Agar media lack the necessary components and environment needed for influenza virus replication. In laboratory animals, such as mice, the viruses can replicate and cause infection. Embryonated eggs provide a suitable environment for influenza virus growth and isolation due to the presence of necessary nutrients and factors. Cell cultures offer controlled conditions for virus propagation and study, making them a valuable tool in influenza virus research.

Correct Answer: A

Rationale: The correct answer is A: Cell wall rigidity. Teichoic acid in Gram-positive bacteria helps to maintain the structural integrity of the cell wall by providing rigidity and stability. It also plays a role in regulating the movement of ions across the cell membrane. Choices B, C, and D are incorrect because teichoic acid is not involved in energy production, spore formation, or motility in bacteria. It primarily functions in cell wall maintenance and integrity.

Correct Answer: C

Rationale: The correct answer is C: Escherichia coli. E. coli is the most common cause of acute cystitis. It typically presents with leukocytes and gram-negative bacilli in the urine. The green pigment production indicates pyocyanin, which is a characteristic of E. coli. Pseudomonas aeruginosa (A) can also produce green pigment, but it is less common in urinary tract infections. Proteus mirabilis (B) is associated with a swarming motility pattern on agar, not mucous colonies. Klebsiella pneumoniae (D) is associated with pneumonia and other respiratory infections, not cystitis.