Correct Answer: A

Rationale: Step-by-step rationale: 1. Tularemia is caused by Francisella tularensis, a highly infectious bacterium. 2. Francisella tularensis is the only pathogen specifically known to cause tularemia. 3. The other choices (Bordetella pertussis, Brucella suis, Legionella sp.) are not associated with tularemia. 4. Therefore, the correct answer is A - Francisella tularensis.

Correct Answer: D

Rationale: The correct answer is D because all three viruses, Vaccinia virus, Smallpox virus, and Cowpox virus, belong to the genus Orthopoxvirus. They share similar characteristics and genetic makeup. Vaccinia virus is used as a vaccine against smallpox, Smallpox virus causes smallpox disease, and Cowpox virus can infect both animals and humans. Choice A, B, and C are incorrect because each of these viruses is a member of the Orthopoxvirus genus.

Correct Answer: D

Rationale: The correct answer is D: Loefler. This staining method is used for identifying Corynebacterium diphtheriae, the causative agent of diphtheria. It involves using methylene blue and basic fuchsin to stain the bacteria. The yellow bacilli with blue grains at their ends indicate the characteristic appearance of C. diphtheriae when stained using the Loefler method. Choice A: Ziehl-Nielsen is used for acid-fast bacteria like Mycobacterium tuberculosis. Choice B: Kozlovsky is not a standard staining method in microbiology. Choice C: Romanovsky is used for blood smears to identify malaria parasites.

Correct Answer: B

Rationale: The Ziehl-Neelsen stain is specifically designed to detect acid-fast bacteria like Mycobacterium tuberculosis. It utilizes a carbol fuchsin dye that binds to the high lipid content in the cell wall of acid-fast bacteria, making them appear red under a microscope. This staining method is crucial for the diagnosis of tuberculosis and leprosy. Gram-positive bacteria (Choice A) are not detected by this stain since they have a different cell wall structure. Capsulated bacteria (Choice C) and fungal spores (Choice D) also have distinct characteristics that are not targeted by the Ziehl-Neelsen stain.

Correct Answer: B

Rationale: The correct answer is B: caliciviruses. Caliciviruses are known to be (+) ssRNA viruses that cause gastroenteritis in a wide range of animals, including birds, cats, dogs, pigs, sheep, cows, and humans. These viruses are highly contagious and can spread through contaminated food or water. Rotaviruses (A) are also a common cause of gastroenteritis, but they are not exclusively isolated from the mentioned animals. Adenoviruses (C) typically cause respiratory or eye infections, not gastroenteritis. Noroviruses (D) are also a major cause of gastroenteritis in humans, but they are not commonly found in animals other than humans.