There is a suspicion of active tuberculosis development in patient. The doctor has appointed Mantoux test to make a diagnosis. What immunobiological agent has to be administered?

Correct Answer: A

Rationale: The correct answer is A: Tuberculine. The Mantoux test involves injecting tuberculin, a protein derivative of Mycobacterium tuberculosis, under the skin. This test helps determine if a person has been exposed to the bacteria that cause TB. Administering tuberculin is crucial for the Mantoux test as it triggers a delayed-type hypersensitivity reaction in individuals previously exposed to TB. Choice B (BCG vaccine) is incorrect because BCG does not interfere with the Mantoux test results. Choice C (DPT vaccine) is incorrect as it is used for immunization against diphtheria, pertussis, and tetanus, not for diagnosing TB. Choice D (Tularin test) is incorrect as it is not a recognized immunobiological agent for TB diagnosis.

Correct Answer: D

Rationale: The correct answer is D because during the incubation period of Salmonella Typhi infection, the bacteria multiply in the macrophages of the Peyer's patches and solitary follicles. This is supported by the fact that S. Typhi is an intracellular pathogen that targets the lymphoid tissues of the intestines. Macrophages in these areas serve as the primary host cells for the bacteria, allowing for their replication and evasion of the host immune response. Choices A, B, and C are incorrect because S. Typhi does not primarily multiply in the small intestine enterocytes, the lumen of the small intestine, or hepatocytes during the initial stages of infection.

Correct Answer: C

Rationale: The correct answer is C: Staphylococcus saprophyticus. The rationale is that Staphylococcus saprophyticus is the only one among the listed species that is known to be resistant to novobiocin. This resistance is due to the specific mechanism of action of novobiocin, which targets DNA gyrase, and Staphylococcus saprophyticus possesses an altered DNA gyrase enzyme that renders it resistant to the antibiotic. Staphylococcus aureus and Staphylococcus epidermidis are susceptible to novobiocin, making choices A and B incorrect. Choice D, "none of the listed," is also incorrect as Staphylococcus saprophyticus is indeed resistant to novobiocin.

Correct Answer: A

Rationale: The correct answer is A: Leptospira interrogans. Darkfield microscopy is used to visualize spirochetes, which are spiral-shaped bacteria. Leptospira is a spirochete bacterium that causes leptospirosis, which presents with jaundice, muscle aching, chill, and nosebleeds. Leptospira is commonly transmitted through contact with contaminated water or soil. B: Borrelia dutlonii causes Lyme disease, which is transmitted by ticks and does not typically present with jaundice or nosebleeds. C: Calymmatobacterium granulomatis causes granuloma inguinale, a sexually transmitted infection that does not present with the symptoms described. D: Bartonella bacilloformis causes Carrion's disease, which presents with fever, anemia, and skin lesions but not jaundice or nosebleeds.

Correct Answer: A

Rationale: The correct answer is A: Bordetella. The presence of mercury-drop like colonies on Bordet-Gengou agar indicates Bordetella pertussis. The small ovoid gram-negative bacilli sized 1-3 microns are characteristic of Bordetella species. Catarrhal symptoms are typical of pertussis caused by Bordetella pertussis. Summary of other choices: B: Corynebacteria do not typically exhibit mercury-drop like colonies or small ovoid shapes. C: Mycobacteria are acid-fast bacilli and do not grow on Bordet-Gengou agar. D: Meningococcus is a gram-negative diplococcus and does not match the description provided.

Correct Answer: D

Rationale: The correct answer is D: polyomavirus infections. Polyomavirus infections do not typically recur during pregnancy because once the virus infects the body, it remains latent without causing recurrent infections. In contrast, choices A, B, and C can recur during pregnancy due to their ability to reactivate from latency or reinfect the host. Epstein-Barr virus infections (choice A) can lead to infectious mononucleosis, herpes simplex virus infections (choice B) can cause recurrent genital or oral herpes outbreaks, and streptococcal infections (choice C) can result in recurrent throat or skin infections.

Correct Answer: A

Rationale: The correct answer is A: Bacillus anthracis. Bacillus anthracis is the likely causative agent as it is a Gram-positive rod-shaped bacterium known to cause ulcerated lesions with associated fever and malaise. Anthrax typically presents with these symptoms and fits the description given in the question. Clostridium perfringens (Choice B) causes gas gangrene and typically presents with different symptoms. Clostridium tetani (Choice C) causes tetanus and is not associated with ulcerated lesions. Bacillus cereus (Choice D) is known for causing food poisoning and does not typically present with the symptoms described in the question.

Correct Answer: A

Rationale: The correct answer is A: Nystatin. Nystatin is an antifungal medication specifically used to treat candida infections. It works by disrupting the cell membrane of the fungus, leading to its death. Penicillin (B) is an antibiotic effective against bacterial infections, not fungal. Streptomycin (C) is also an antibiotic used for bacterial infections. Isoniazid (D) is used to treat tuberculosis, not candida infections. Therefore, Nystatin is the best choice for treating candida infections due to its antifungal properties.

Correct Answer: A

Rationale: The correct answer is A: Neutralization reaction. This is the most appropriate serological test to reveal botulinum toxin because it involves mixing the patient's serum with known botulinum antitoxin. If the patient's serum contains botulinum toxin, it will neutralize the antitoxin, leading to a lack of toxin-neutralizing ability. The other choices are incorrect because agglutination reaction involves clumping of cells, not suitable for detecting botulinum toxin; Bordet-Gengou test is used to detect antibodies in pertussis infection, not botulinum toxin; and precipitation reaction involves the formation of a solid when two solutions are mixed, not ideal for detecting botulinum toxin.