There is a suspicion of active tuberculosis development in patient. The doctor has appointed Mantoux test to make a diagnosis. What immunobiological agent has to be administered?

Correct Answer: A

Rationale: The correct answer is A: Tuberculine. The Mantoux test involves injecting tuberculin, a protein derivative of Mycobacterium tuberculosis, under the skin. This test helps determine if a person has been exposed to the bacteria that cause TB. Administering tuberculin is crucial for the Mantoux test as it triggers a delayed-type hypersensitivity reaction in individuals previously exposed to TB. Choice B (BCG vaccine) is incorrect because BCG does not interfere with the Mantoux test results. Choice C (DPT vaccine) is incorrect as it is used for immunization against diphtheria, pertussis, and tetanus, not for diagnosing TB. Choice D (Tularin test) is incorrect as it is not a recognized immunobiological agent for TB diagnosis.

Correct Answer: B

Rationale: The correct answer is B: Diarrhea. Brucellosis typically presents with symptoms such as arthralgia, headache, and undulating fever, but diarrhea is not a common symptom associated with the disease. Brucellosis is primarily characterized by systemic symptoms like fever, joint pain, and headache due to the bacterial infection. Diarrhea is more commonly seen in gastrointestinal infections rather than brucellosis. Therefore, the absence of diarrhea as a symptom in brucellosis helps in differentiating it from other diseases with similar clinical presentations.

Correct Answer: A

Rationale: Rationale: Vibrio cholerae produces the exotoxin responsible for severe diarrhea. It causes cholera by disrupting the normal functioning of the intestines. Clostridium botulinum causes botulism, not diarrhea. Streptococcus pyogenes causes strep throat and skin infections. Salmonella enterica causes food poisoning, not severe diarrhea. Therefore, the correct answer is A.

Correct Answer: A

Rationale: The correct answer is A: Toxoplasma. Toxoplasma gondii is a protozoan parasite that can infect the brain and cause vision loss. The description of half-moon-shaped organisms with a pointed end matches the appearance of Toxoplasma under the microscope. Toxoplasma is known to cause a condition called toxoplasmosis, which can lead to neurological symptoms such as vision loss. Leishmania, Lamblia, Amoeba, and Trichomonad are not known to cause brain infections or vision loss, making them incorrect choices.

Correct Answer: B

Rationale: The correct answer is B: Facultative anaerobes. Facultative anaerobes can survive in both aerobic and anaerobic environments. In aerobic conditions, they use oxygen for energy production, while in anaerobic conditions, they can switch to fermentation or anaerobic respiration. A: Obligate aerobes require oxygen to survive and cannot survive in anaerobic environments. C: Obligate anaerobes cannot survive in the presence of oxygen and only thrive in anaerobic conditions. D: Microaerophiles require low levels of oxygen to survive and cannot thrive in fully aerobic or anaerobic conditions.

Correct Answer: A

Rationale: The correct answer is A: Leptospira interrogans. Darkfield microscopy is used to visualize spirochetes, which are spiral-shaped bacteria. Leptospira is a spirochete bacterium that causes leptospirosis, which presents with jaundice, muscle aching, chill, and nosebleeds. Leptospira is commonly transmitted through contact with contaminated water or soil. B: Borrelia dutlonii causes Lyme disease, which is transmitted by ticks and does not typically present with jaundice or nosebleeds. C: Calymmatobacterium granulomatis causes granuloma inguinale, a sexually transmitted infection that does not present with the symptoms described. D: Bartonella bacilloformis causes Carrion's disease, which presents with fever, anemia, and skin lesions but not jaundice or nosebleeds.

Correct Answer: C

Rationale: Rabbit fever, or tularemia, is caused by Francisella tularensis. This bacterium is known to infect a wide range of animals, including rabbits. The other choices, Bartonella henselae (causes cat scratch fever), Spirillum minus (causes rat bite fever), and Yersinia pestis (causes plague) are not associated with rabbit fever. Therefore, the correct answer is C based on the specific pathogen responsible for causing tularemia.

Correct Answer: B

Rationale: The correct answer is B: serratia spp. This is because serratia spp. are not typically found in the normal skin microflora. Normal skin microflora primarily consist of bacteria such as diphtheroids, alpha-hemolytic streptococci, and epidermidis. Serratia spp. are more commonly associated with healthcare-associated infections rather than being a part of the skin's natural bacterial flora. Therefore, choice B is the correct answer. Choices A, C, and D are incorrect as they are commonly found in the normal skin microflora.

Correct Answer: C

Rationale: The correct answer is C because Gram staining after culture on Loeffler's medium or tellurite-containing medium will show the characteristic Chinese letter arrangement of Corynebacterium diphtheriae. This arrangement consists of metachromatic granules that stain more deeply than the rest of the cell. This specific staining pattern is a key diagnostic feature of C. diphtheriae in microbiological diagnosis of diphtheria. Choice A is incorrect because simply identifying the isolate as C. diphtheriae is not sufficient for diagnosis. Choice B is incorrect as C. diphtheriae can be stained via Gram staining despite its small cell size. Choice D is incorrect as option C is indeed correct for microbiological diagnosis of diphtheria.