There is a suspicion of active tuberculosis development in patient. The doctor has appointed Mantoux test to make a diagnosis. What immunobiological agent has to be administered?

Correct Answer: A

Rationale: The correct answer is A: Tuberculine. The Mantoux test involves injecting tuberculin, a protein derivative of Mycobacterium tuberculosis, under the skin. This test helps determine if a person has been exposed to the bacteria that cause TB. Administering tuberculin is crucial for the Mantoux test as it triggers a delayed-type hypersensitivity reaction in individuals previously exposed to TB. Choice B (BCG vaccine) is incorrect because BCG does not interfere with the Mantoux test results. Choice C (DPT vaccine) is incorrect as it is used for immunization against diphtheria, pertussis, and tetanus, not for diagnosing TB. Choice D (Tularin test) is incorrect as it is not a recognized immunobiological agent for TB diagnosis.

Correct Answer: A

Rationale: 1. Gram-positive cocci in clusters suggest Staphylococcus species. 2. Catalase-positive and coagulase-positive characteristics match with Staphylococcus aureus. 3. Staphylococcus aureus is a common cause of wound infections due to its virulence factors. 4. Other choices are incorrect: B) S. epidermidis is coagulase-negative and a common contaminant, C) S. pyogenes is catalase-negative, D) Enterococcus faecalis is not typically associated with wound infections.

Correct Answer: A

Rationale: The correct answer is A: Neutralization reaction. This is the most appropriate serological test to reveal botulinum toxin because it involves mixing the patient's serum with known botulinum antitoxin. If the patient's serum contains botulinum toxin, it will neutralize the antitoxin, leading to a lack of toxin-neutralizing ability. The other choices are incorrect because agglutination reaction involves clumping of cells, not suitable for detecting botulinum toxin; Bordet-Gengou test is used to detect antibodies in pertussis infection, not botulinum toxin; and precipitation reaction involves the formation of a solid when two solutions are mixed, not ideal for detecting botulinum toxin.

Correct Answer: C

Rationale: The correct answer is C: Staphylococcus saprophyticus. The rationale is that Staphylococcus saprophyticus is the only one among the listed species that is known to be resistant to novobiocin. This resistance is due to the specific mechanism of action of novobiocin, which targets DNA gyrase, and Staphylococcus saprophyticus possesses an altered DNA gyrase enzyme that renders it resistant to the antibiotic. Staphylococcus aureus and Staphylococcus epidermidis are susceptible to novobiocin, making choices A and B incorrect. Choice D, "none of the listed," is also incorrect as Staphylococcus saprophyticus is indeed resistant to novobiocin.

Correct Answer: A

Rationale: The correct answer is A: tRNA. Anticodons are sequences of nucleotides found on tRNA molecules that complement the codons on mRNA during translation. tRNA molecules carry specific amino acids to the ribosome based on the codon-anticodon pairing. Option B, rRNA, forms the structure of the ribosome and is not directly involved in anticodon recognition. Option C, mRNA, contains codons but not anticodons. Option D, ribosomes, are the site of protein synthesis but do not contain the anticodons themselves.

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. Step-by-step rationale: 1. Gram-negative rods: E. coli is a Gram-negative rod bacterium. 2. Fermented lactose: E. coli is known to ferment lactose. 3. Common cause of diarrhea: E. coli is a common causative agent of infectious diarrhea. Summary: B: Salmonella typhi causes typhoid fever, not typically associated with lactose fermentation. C: Shigella dysenteriae causes dysentery, not known for lactose fermentation. D: Proteus vulgaris is not a common cause of infectious diarrhea and does not typically ferment lactose.

Correct Answer: C

Rationale: The correct answer is C: after repeated exposure for days. Subacute toxic effects typically manifest after a period of time that is longer than immediate (A) or acute (B) effects but shorter than chronic effects (D). This duration of days aligns with the definition of subacute, which refers to effects that occur gradually over a relatively short time frame. It is important to differentiate between the timing of toxic effects to accurately assess the potential harm caused by a substance. Choices A, B, and D are incorrect because they do not align with the characteristic time frame associated with subacute toxic effects.

Correct Answer: A

Rationale: The correct answer is A because it accurately describes the major morphological forms of bacteria. Cocci are spherical, rod-shaped bacteria are called bacilli, and curved bacteria are spirilla. Staphylococci, streptococci, and diplococci are specific types of cocci, not major morphological forms. Basicili and spiralis are not common morphological forms of bacteria. Option D is incorrect as the other choices do represent major morphological forms of bacteria.

Correct Answer: A

Rationale: Step 1: Gram-positive cocci in grape-like clusters suggest Staphylococcus. Step 2: Catalase-positive indicates Staphylococcus (negative for Streptococcus). Step 3: Coagulase-positive is specific for Staphylococcus aureus. Summary: B: Streptococcus pyogenes is catalase-negative. C: Enterococcus faecalis does not form grape-like clusters. D: Micrococcus luteus is catalase-positive but not coagulase-positive.