ATI RN
Foundations in Microbiology Test Bank Questions
Question 1 of 5
There are several cases of children from boarding school suffering from sore throat. Microscopy of tonsil smears stained according Neisser method has revealed thin yellow bacilli with dark brown grains on their ends placed in the shape of Roman numeral five. What infection can be suspected in this case?
Correct Answer: A
Rationale: Rationale for Correct Answer A (Diphtheria): 1. Thin yellow bacilli with dark brown grains on ends suggest Corynebacterium diphtheriae, characteristic of diphtheria. 2. Roman numeral five shape is typical of diphtheria bacilli. 3. Diphtheria causes sore throat and can spread in boarding schools. 4. Neisser staining method is used for diphtheria bacilli. Summary of Incorrect Choices: B: Scarlet fever is caused by Streptococcus pyogenes and presents with rash, not yellow bacilli. C: Listeriosis is caused by Listeria monocytogenes, not consistent with the described findings. D: Tonsillitis can be caused by various bacteria or viruses, but the specific characteristics described do not match.
Question 2 of 5
Bacterial spores are used for:
Correct Answer: C
Rationale: The correct answer is C: Survival. Bacterial spores are specialized structures that bacteria form to survive harsh conditions such as extreme temperatures or lack of nutrients. Spores are not used for protection against phagocytosis (A) as they are dormant structures and not actively involved in defense mechanisms. They are not for reproduction (B) as spores are a form of bacterial reproduction but not the primary purpose of spore formation. Spores do not serve as nutrient storage (D) as they are mainly used for survival during unfavorable conditions.
Question 3 of 5
A 60-year-old patient was hospitalized to the surgical department because of infection caused by blue pus bacillus (Pseudomonas aeruginosa) which is sensative to penicillin antibiotics. Indicate which of the given penicillins has marked activity to the Pseudomonas aeruginosa?
Correct Answer: A
Rationale: The correct answer is A: Carbenicillin disodium. Carbenicillin is a semisynthetic penicillin that has marked activity against Pseudomonas aeruginosa. This is due to its extended spectrum of activity and increased stability against beta-lactamases produced by Pseudomonas aeruginosa. Carbenicillin is commonly used to treat infections caused by this bacterium. Choice B: Benzylpenicillin (Penicillin G) has limited activity against Pseudomonas aeruginosa. Choice C: Methicillin is not effective against Pseudomonas aeruginosa. Choice D: Oxacillin also has limited activity against Pseudomonas aeruginosa. In summary, Carbenicillin is the correct choice due to its marked activity against Pseudomonas aeruginosa compared to the other penicillins listed.
Question 4 of 5
All statements about plasmids are correct except:
Correct Answer: C
Rationale: The correct answer is C because plasmids do not encode essential bacterial properties. They are non-essential, extra-chromosomal DNA molecules that can replicate independently within bacterial cells. Plasmids mainly contain genes for antibiotic resistance, genetic transfer, and other specific functions. Choice A is correct as many plasmids do carry antibiotic resistance genes. Choice B is also accurate as plasmids are indeed smaller, circular, and exist outside the bacterial chromosome. Choice D is true as plasmids can facilitate bacterial conjugation, allowing the transfer of genetic material between bacteria.
Question 5 of 5
During sanitary and bacteriological testing of water with the membrane filter technique there were revealed two red colonies on a membrane filter (Endo agar) through which 500 ml of water was filtred. Calculate the coli index and coli titer of the analyzed water:
Correct Answer: C
Rationale: The correct answer is C (coli index of 250 and coli titer of 4). The coli index is calculated by multiplying the number of colonies by the reciprocal of the volume filtered, which gives 2 colonies x (1/0.5 L) = 4. The coli titer is obtained by dividing the volume filtered by the number of colonies, which gives 0.5 L / 2 colonies = 0.25 L or 250 ml. This indicates the concentration of coliform bacteria in the water sample. Choice A (4 and 250) is incorrect because it has the correct coli index but the values are reversed for coli index and coli titer. Choice B (2 and 500) is incorrect as it provides the correct coli titer value but the values are reversed for coli index and coli titer. Choice D (500 and 2) is incorrect because it reverses the values for both coli index and coli titer.
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