The stain used to identify bacteria with a large amount of peptidoglycan in their cell walls is the:

Correct Answer: C

Rationale: The correct answer is C: Negative stain. Negative staining uses acidic dyes which are repelled by the bacterial cell wall components, highlighting bacteria with a large amount of peptidoglycan. Acid-fast stain (A) is used to identify Mycobacterium species with waxy cell walls. Gram stain (B) differentiates bacteria into Gram-positive and Gram-negative based on cell wall composition. Option D is a duplicate of option C and is incorrect.

Correct Answer: A

Rationale: Rationale: A: Early application of gentamicin is not recommended as a specific treatment for C. difficile-associated post antibiotic diarrhea because it does not have efficacy against C. difficile. B: Metronidazole is recommended as first-line treatment for mild to moderate C. difficile infection. C: Stopping antibiotic treatment and observing the clinical response for 48 hours is not a recommended strategy as prompt treatment is crucial in C. difficile infection. D: Vancomycin per os is an appropriate treatment for severe C. difficile infection. In summary, A is incorrect because gentamicin is not effective against C. difficile, while B, C, and D are potential treatment strategies based on severity of the infection.

Correct Answer: A

Rationale: The correct answer is A: Entamoeba histolytica. This is because Entamoeba histolytica is known to cause amebic dysentery, leading to chronic diarrhea. The presence of cysts with four nuclei in the stool sample is characteristic of Entamoeba histolytica. Giardia lamblia (choice B) is known to cause diarrhea but does not present with cysts containing four nuclei. Balantidium coli (choice C) is a ciliated protozoan that can cause diarrhea, but it does not have cysts with four nuclei. Trichomonas hominis (choice D) is a non-pathogenic protozoan commonly found in the human colon and is not associated with causing chronic diarrhea or cysts with four nuclei.

Correct Answer: A

Rationale: The correct answer is A, Clostridium botulinum. This bacterium produces botulinum toxin, leading to botulism. It is commonly found in soil and improperly canned food. Clostridium tetani causes tetanus, Staphylococcus aureus causes food poisoning, and Salmonella enterica causes salmonellosis. Therefore, A is correct due to its association with botulism, while the other choices are incorrect for causing different diseases.

Correct Answer: A

Rationale: The correct choice is A: Binding to IgE fixed to mast cells. The Besredka desensitization method involves injecting small, gradually increasing doses of the allergen to induce tolerance and prevent anaphylactic shock. This process works by binding the allergen to IgE antibodies fixed to mast cells, which prevents the release of histamine and other inflammatory mediators that trigger allergic reactions. This desensitization process helps the immune system become less reactive to the allergen over time. Incorrect choices: B: Inhibited synthesis of mast cells mediators - This choice does not reflect the mechanism of the Besredka desensitization method, which involves binding to IgE on mast cells. C: Stimulation of the immunological antigen tolerance - While this may sound plausible, the Besredka method specifically targets the IgE-mast cell interaction. D: Stimulation of antigen-specific IgG synthesis - While IgG antibodies may play a role in desensitization, the

Correct Answer: A

Rationale: The correct answer is A: T-lymphocytes. Cell-mediated immunity involves the activation of T-lymphocytes, which play a crucial role in recognizing and killing infected cells. T-lymphocytes include cytotoxic T cells, helper T cells, and regulatory T cells, all of which are involved in different aspects of cell-mediated immunity. Monocytes (choice B) are a type of white blood cell involved in phagocytosis but are not the main mediators of cell-mediated immunity. B-lymphocytes (choice C) are involved in humoral immunity, producing antibodies, not cell-mediated immunity. Choice D (A+B+C) is incorrect because cell-mediated immunity is mainly mediated by T-lymphocytes.

Correct Answer: A

Rationale: The correct answer is A because penicillins and cephalosporins inactivate transpeptidase activity of penicillin binding proteins, leading to disruption of bacterial cell wall synthesis. This mechanism makes them bactericidal, not bacteriostatic (choice B). Choice C is incorrect as penicillins and cephalosporins target cell wall synthesis, not the cytoplasmic membrane. Choice D is incorrect as option A is the correct statement.

Correct Answer: B

Rationale: The correct answer is B: Clostridium tetani. Clostridium tetani produces tetanospasmin, a neurotoxin causing tetanus. It enters the body through wounds and affects the nervous system. A: Clostridium botulinum produces botulinum toxin causing botulism, not tetanus. C: Streptococcus pneumoniae causes pneumonia, not tetanus. D: Mycobacterium tuberculosis causes tuberculosis, not tetanus.

Correct Answer: C

Rationale: The correct answer is C: Leishmaniasis. The description of thin microorganisms with 12-14 uniform tendrils, 10-13 micrometers in length, and pale pink color matches the characteristics of Leishmania parasites seen in Giemsa-stained samples. Leishmania parasites are known to have a distinctive appearance with pointed tips and a specific size range. Syphilis (choice A) is caused by Treponema pallidum, which appears as tightly coiled spirochetes, not tendrils. Trypanosomiasis (choice B) is caused by Trypanosoma parasites, which have a different morphology and staining pattern. Relapsing fever (choice D) is caused by Borrelia recurrentis, which does not match the description given. Therefore, the correct choice is C as the description aligns closely with the characteristic features of Leishmania parasites.