The stain used to identify bacteria with a large amount of peptidoglycan in their cell walls is the:

Correct Answer: C

Rationale: The correct answer is C: Negative stain. Negative staining uses acidic dyes which are repelled by the bacterial cell wall components, highlighting bacteria with a large amount of peptidoglycan. Acid-fast stain (A) is used to identify Mycobacterium species with waxy cell walls. Gram stain (B) differentiates bacteria into Gram-positive and Gram-negative based on cell wall composition. Option D is a duplicate of option C and is incorrect.

Correct Answer: C

Rationale: The correct answer is C because diphtheria exotoxin is absorbed into the underlying healthy tissues and spreads throughout the body. This is due to the toxin's ability to enter the bloodstream and target various organs, causing systemic effects. Choice A is incorrect as the toxin does not stay localized at the site of infection. Choice B is incorrect as diphtheria exotoxin does not directly affect neuro-muscular transmission. Choice D is also incorrect as the toxin does spread throughout the body.

Correct Answer: A

Rationale: The correct answer is A, Clostridium botulinum. This bacterium produces botulinum toxin, leading to botulism. It is commonly found in soil and improperly canned food. Clostridium tetani causes tetanus, Staphylococcus aureus causes food poisoning, and Salmonella enterica causes salmonellosis. Therefore, A is correct due to its association with botulism, while the other choices are incorrect for causing different diseases.

Correct Answer: A

Rationale: The correct answer is A: Toxin production test. Rationale: 1. Diphtheria is caused by a toxin produced by Corynebacterium diphtheriae. 2. To confirm if the carrier is dangerous, we need to test if they are producing the toxin. 3. The toxin production test directly assesses if the bacteria are producing the harmful toxin. 4. Positive results indicate the carrier is dangerous and can transmit the disease to others. Summary of other choices: B: Mouse neutralization test - Used to detect diphtheria antitoxin levels in blood, not to confirm if the carrier is dangerous. C: Immunofluorescence test - Used to detect specific antigens or antibodies, not directly related to confirming the carrier's danger. D: Complement fixation test - Measures immune response, not specific to toxin production confirmation.

Correct Answer: B

Rationale: The main function of bacterial pili is to facilitate conjugation and adhesion. Pili are hair-like appendages on bacteria that help in cell-to-cell interactions. They play a crucial role in bacterial conjugation by allowing the transfer of genetic material between cells. Additionally, pili help bacteria adhere to surfaces or host cells, aiding in colonization and biofilm formation. Choices A and D are incorrect because protein synthesis and toxin secretion are not the primary functions of pili. Choice C is incorrect as bacterial motility is mainly mediated by flagella, not pili.

Correct Answer: A

Rationale: The correct answer is A: Gram staining. This method is used to differentiate bacteria based on their cell wall composition. In cholera, the causative agent is Vibrio cholerae, which is Gram-negative. By performing Gram staining, the bacteria will appear pink/red under the microscope, confirming the presence of Gram-negative bacteria. Choice B: Neisser's staining is used for staining capsules of bacteria, not suitable for confirming cholera diagnosis. Choice C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for confirming cholera diagnosis. Choice D: Burri-Gins staining is not a standard staining method in microbiology and is not relevant for confirming cholera diagnosis.

Correct Answer: D

Rationale: The correct answer is D: Structure of cell wall. When studying a bacterial smear stained by the Ozheshko method, the focus is on the cell wall structure. This staining method highlights the cell wall morphology, allowing for detailed examination. Flagella, spores, and capsules are not typically visualized using this staining method. Flagella require special staining techniques, spores are usually observed through specific staining methods like Schaeffer-Fulton, and capsules are not easily visualized with the Ozheshko staining method. Therefore, the structure of the cell wall is the most relevant feature to study in this context.

Correct Answer: A

Rationale: The correct answer is A because penicillins and cephalosporins inactivate transpeptidase activity of penicillin binding proteins, leading to disruption of bacterial cell wall synthesis. This mechanism makes them bactericidal, not bacteriostatic (choice B). Choice C is incorrect as penicillins and cephalosporins target cell wall synthesis, not the cytoplasmic membrane. Choice D is incorrect as option A is the correct statement.

Correct Answer: A

Rationale: Aminoglycosides primarily inhibit protein synthesis by binding to the 30S ribosomal subunit in bacteria. This action leads to misreading of mRNA and ultimately disrupts the translation process, resulting in faulty protein production and bacterial cell death. Incorrect choices: B: Aminoglycosides do not inhibit DNA replication. C: Aminoglycosides do not disrupt cell membrane integrity. D: Aminoglycosides do not inhibit folic acid synthesis. In summary, the correct choice (A) aligns with the known mechanism of action of aminoglycosides, making it the most appropriate answer.