ATI RN
microbiology test bank Questions
Question 1 of 9
The stain used to identify bacteria with a large amount of peptidoglycan in their cell walls is the:
Correct Answer: C
Rationale: The correct answer is C: Negative stain. Negative staining uses acidic dyes which are repelled by the bacterial cell wall components, highlighting bacteria with a large amount of peptidoglycan. Acid-fast stain (A) is used to identify Mycobacterium species with waxy cell walls. Gram stain (B) differentiates bacteria into Gram-positive and Gram-negative based on cell wall composition. Option D is a duplicate of option C and is incorrect.
Question 2 of 9
A 5-year-old kindergartener has diphtheria. To find the carriers of the disease among the kindergarten staff, samples of pharyngeal mucus were obtained from the employers. One of the employers had gram-positive bacilli in her sample. They were situated at an angle to each other and colored unevenly, when stained according to Loeffler. What method can confirm that this carrier is dangerous to other people?
Correct Answer: A
Rationale: The correct answer is A: Toxin production test. Rationale: 1. Diphtheria is caused by a toxin produced by Corynebacterium diphtheriae. 2. To confirm if the carrier is dangerous, we need to test if they are producing the toxin. 3. The toxin production test directly assesses if the bacteria are producing the harmful toxin. 4. Positive results indicate the carrier is dangerous and can transmit the disease to others. Summary of other choices: B: Mouse neutralization test - Used to detect diphtheria antitoxin levels in blood, not to confirm if the carrier is dangerous. C: Immunofluorescence test - Used to detect specific antigens or antibodies, not directly related to confirming the carrier's danger. D: Complement fixation test - Measures immune response, not specific to toxin production confirmation.
Question 3 of 9
The actual genetic makeup of an organism is referred to as its
Correct Answer: B
Rationale: The correct answer is B: genotype. The genotype refers to the actual genetic makeup of an organism, including all the genes and alleles present in its DNA. This determines the traits and characteristics of an organism. Phenotype (A) is the observable physical or biochemical characteristics of an organism. Conjugation (C) is a process in bacteria involving the transfer of genetic material. Construct (D) is not a term related to genetics. Therefore, the correct choice is B as it specifically refers to the genetic composition of an organism.
Question 4 of 9
Chlorhexidine
Correct Answer: A
Rationale: Step 1: Chlorhexidine belongs to the group of biguanides, making choice A accurate. Step 2: It is commonly used for disinfection of hands, skin, and mucous membranes. Step 3: It has a broad spectrum of antimicrobial activity, making it widely applicable. Summary: Choice B is incorrect as chlorhexidine is not classified as an alcohol. Choice C is incorrect as chlorhexidine has various applications. Choice D is incorrect as chlorhexidine is generally safe for use with low toxicity.
Question 5 of 9
The genome of family orthomyxoviridae is represented by:
Correct Answer: D
Rationale: The correct answer is D: Single stranded segmented (-) RNA. Orthomyxoviridae family includes influenza viruses, which have a genome composed of single-stranded segmented negative-sense RNA. This specific structure allows for efficient replication and transcription within host cells. Choice A is incorrect as orthomyxoviruses do not have a double-stranded DNA genome. Choice B is incorrect because orthomyxoviruses have a negative-sense RNA genome, not a positive-sense RNA genome. Choice C is incorrect as orthomyxoviruses do not have a double-stranded RNA genome.
Question 6 of 9
During an outbreak of cholera, a fecal smear revealed Gram-negative, comma-shaped, motile bacteria. What staining method would best confirm the diagnosis?
Correct Answer: A
Rationale: The correct answer is A: Gram staining. This method is used to differentiate bacteria based on their cell wall composition. In cholera, the causative agent is Vibrio cholerae, which is Gram-negative. By performing Gram staining, the bacteria will appear pink/red under the microscope, confirming the presence of Gram-negative bacteria. Choice B: Neisser's staining is used for staining capsules of bacteria, not suitable for confirming cholera diagnosis. Choice C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for confirming cholera diagnosis. Choice D: Burri-Gins staining is not a standard staining method in microbiology and is not relevant for confirming cholera diagnosis.
Question 7 of 9
In a microslide of the patient's regional lymph node stained with Giemsa method a doctor detected thin microorganisms with 12-14 uniform tendrils with pointed tips, 10-13 micrometers in length, pale pink in color. In this case they can be identified as infectious agents of the following disease:
Correct Answer: C
Rationale: The correct answer is C: Leishmaniasis. The description of thin microorganisms with 12-14 uniform tendrils, 10-13 micrometers in length, and pale pink color matches the characteristics of Leishmania parasites seen in Giemsa-stained samples. Leishmania parasites are known to have a distinctive appearance with pointed tips and a specific size range. Syphilis (choice A) is caused by Treponema pallidum, which appears as tightly coiled spirochetes, not tendrils. Trypanosomiasis (choice B) is caused by Trypanosoma parasites, which have a different morphology and staining pattern. Relapsing fever (choice D) is caused by Borrelia recurrentis, which does not match the description given. Therefore, the correct choice is C as the description aligns closely with the characteristic features of Leishmania parasites.
Question 8 of 9
A patient with pneumonia had sputum cultured revealing Gram-positive diplococci. The bacteria were catalase-negative and alpha-hemolytic. What is the most likely causative agent?
Correct Answer: A
Rationale: The correct answer is A: Streptococcus pneumoniae. The given characteristics match this bacterium: Gram-positive diplococci, catalase-negative, and alpha-hemolytic. S. pneumoniae is a common cause of community-acquired pneumonia. Other choices are incorrect because Staphylococcus aureus is catalase-positive, Enterococcus faecalis is not alpha-hemolytic, and Klebsiella pneumoniae is not Gram-positive diplococci.
Question 9 of 9
Those organisms which in the process of evolution failed to develop protection from H O can exist 2 2 only in anaerobic conditions. Which of the following enzymes can break hydrogen peroxide down?
Correct Answer: A
Rationale: The correct answer is A: Peroxidase and catalase. Peroxidase and catalase are enzymes that break down hydrogen peroxide into water and oxygen. Peroxidase helps in breaking down low levels of hydrogen peroxide, while catalase is crucial for breaking down high levels of hydrogen peroxide efficiently. This process is important for organisms to prevent damage from reactive oxygen species. Choices B, C, and D are incorrect: B: Oxygenase and hydroxylase are enzymes involved in different biochemical reactions and do not break down hydrogen peroxide. C: Cytochrome oxidase and cytochrome B5 are involved in electron transport chain reactions and not in breaking down hydrogen peroxide. D: Oxygenase and catalase are not correct as oxygenase is not involved in hydrogen peroxide breakdown, only catalase is.