The primary mode of action of aminoglycosides is to:

Correct Answer: A

Rationale: Aminoglycosides primarily inhibit protein synthesis by binding to the 30S ribosomal subunit in bacteria. This action leads to misreading of mRNA and ultimately disrupts the translation process, resulting in faulty protein production and bacterial cell death. Incorrect choices: B: Aminoglycosides do not inhibit DNA replication. C: Aminoglycosides do not disrupt cell membrane integrity. D: Aminoglycosides do not inhibit folic acid synthesis. In summary, the correct choice (A) aligns with the known mechanism of action of aminoglycosides, making it the most appropriate answer.

Correct Answer: B

Rationale: The main function of bacterial pili is to facilitate conjugation and adhesion. Pili are hair-like appendages on bacteria that help in cell-to-cell interactions. They play a crucial role in bacterial conjugation by allowing the transfer of genetic material between cells. Additionally, pili help bacteria adhere to surfaces or host cells, aiding in colonization and biofilm formation. Choices A and D are incorrect because protein synthesis and toxin secretion are not the primary functions of pili. Choice C is incorrect as bacterial motility is mainly mediated by flagella, not pili.

Correct Answer: D

Rationale: The correct answer is D: Single stranded segmented (-) RNA. Orthomyxoviridae family includes influenza viruses, which have a genome composed of single-stranded segmented negative-sense RNA. This specific structure allows for efficient replication and transcription within host cells. Choice A is incorrect as orthomyxoviruses do not have a double-stranded DNA genome. Choice B is incorrect because orthomyxoviruses have a negative-sense RNA genome, not a positive-sense RNA genome. Choice C is incorrect as orthomyxoviruses do not have a double-stranded RNA genome.

Correct Answer: A

Rationale: The correct answer is A: Isoniazid. Isoniazid is a first-line drug for treating active pulmonary tuberculosis due to its high efficacy and low toxicity. It works by inhibiting the synthesis of mycolic acids in the bacterial cell wall. Sulfalen, Cyclocerine, and Ethionamide are not first-line drugs for tuberculosis. Sulfalen is used for urinary tract infections, Cyclocerine for drug-resistant tuberculosis, and Ethionamide as a second-line drug for multidrug-resistant tuberculosis. Therefore, Isoniazid is the most appropriate initial choice for treating active focal pulmonary tuberculosis.

Correct Answer: A

Rationale: The correct answer is A, Clostridium botulinum. This bacterium produces botulinum toxin, leading to botulism. It is commonly found in soil and improperly canned food. Clostridium tetani causes tetanus, Staphylococcus aureus causes food poisoning, and Salmonella enterica causes salmonellosis. Therefore, A is correct due to its association with botulism, while the other choices are incorrect for causing different diseases.

Correct Answer: D

Rationale: The correct answer is D: Agglutination test type Gruber. This is because the Gruber agglutination test is not typically used for the serological identification of Yersinia pestis. The other options (A, B, C) are commonly used methods for serological identification of Y. pestis: A) Precipitation reaction involves the formation of a visible precipitate when Y. pestis antigen reacts with specific antibodies. B) Indirect hemagglutination test detects antibodies against Y. pestis by measuring the agglutination of red blood cells. C) Immunofluorescence test uses fluorescently labeled antibodies to detect specific antigens of Y. pestis. In summary, the Gruber agglutination test is not a standard method for serological identification of Yersinia pestis, unlike the other options provided.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Anopheles mosquitoes are the primary vectors for transmitting malaria. 2. They prefer stagnant water for breeding, common in tropical regions. 3. Culex mosquitoes primarily spread diseases like West Nile virus. 4. Aedes mosquitoes transmit diseases like Zika and dengue fever. 5. Mansonia mosquitoes are known for transmitting filarial diseases. Therefore, the correct answer is A (Anopheles), as they are specifically associated with spreading malaria.

Correct Answer: C

Rationale: Step 1: The nucleocapsid is the structure containing the viral genome and the protein capsid. Step 2: The genome carries the genetic material of the virus. Step 3: The capsid is the protein coat that encloses and protects the viral genome. Step 4: Therefore, the correct answer is C: Genome and capsid. Summary: A: Capsid and spikes - Incorrect, spikes are not part of the nucleocapsid. B: Genome and envelop - Incorrect, the envelop is not part of the nucleocapsid. D: Capsid and envelope - Incorrect, the envelope is not part of the nucleocapsid.

Correct Answer: B

Rationale: The correct answer is B: Phylum. After class in the classification system comes phylum. The classification system starts with the broadest category, which is the kingdom, followed by phylum, class, order, family, genus, and species. Phylum is a higher level of classification than class, so it comes next in the hierarchy. Domain is a higher level than kingdom, and order is a lower level of classification than class. Therefore, the correct sequence is kingdom, phylum, class, order.