The one-way transfer of genetic material from a donor cell to a recipient cell by direct cell to cell contact is:

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microbiology an introduction 13th edition test bank Questions

Question 1 of 9

The one-way transfer of genetic material from a donor cell to a recipient cell by direct cell to cell contact is:

Correct Answer: B

Rationale: The correct answer is B: Conjugation. Conjugation involves the transfer of genetic material from a donor cell to a recipient cell through direct cell-to-cell contact, typically mediated by a conjugative plasmid. During conjugation, a conjugative pilus forms a bridge between the donor and recipient cells, allowing the transfer of genetic material. A: Replication is the process of copying DNA to produce two identical DNA molecules. It does not involve the transfer of genetic material between cells. C: Transformation is the uptake and incorporation of foreign DNA from the environment by a bacterial cell. It does not require direct cell-to-cell contact. D: Transduction is the transfer of genetic material from one bacterium to another by a bacteriophage vector. It does not involve direct cell-to-cell contact between the donor and recipient cells.

Question 2 of 9

A bacteriological laboratory received a sample of dried fish from an outbreak of food poisoning. Inoculation of the sample on Kitt-Tarozzi medium revealed microorganisms resembling tennis racket. These microorganisms are causative agents of the following disease:

Correct Answer: B

Rationale: Step 1: The description of microorganisms resembling tennis racket indicates Corynebacterium diphtheriae, the causative agent of diphtheria. Step 2: Diphtheria is a bacterial infection that can cause severe respiratory and systemic symptoms. Step 3: Botulism is caused by Clostridium botulinum, not tennis racket-shaped microorganisms. Step 4: Typhoid fever is caused by Salmonella typhi, which does not resemble tennis rackets. Step 5: Salmonellosis is caused by various Salmonella species, which also do not have the described tennis racket appearance.

Question 3 of 9

The one-way transfer of genetic material from a donor cell to a recipient cell by direct cell to cell contact is:

Correct Answer: B

Rationale: The correct answer is B: Conjugation. Conjugation involves the transfer of genetic material from a donor cell to a recipient cell through direct cell-to-cell contact, typically mediated by a conjugative plasmid. During conjugation, a conjugative pilus forms a bridge between the donor and recipient cells, allowing the transfer of genetic material. A: Replication is the process of copying DNA to produce two identical DNA molecules. It does not involve the transfer of genetic material between cells. C: Transformation is the uptake and incorporation of foreign DNA from the environment by a bacterial cell. It does not require direct cell-to-cell contact. D: Transduction is the transfer of genetic material from one bacterium to another by a bacteriophage vector. It does not involve direct cell-to-cell contact between the donor and recipient cells.

Question 4 of 9

What is the role of bacterial plasmids?

Correct Answer: C

Rationale: The correct answer is C: Carry genes for antibiotic resistance. Bacterial plasmids are small, circular DNA molecules that can be transferred between bacteria, carrying genes such as antibiotic resistance. This allows bacteria to survive in the presence of antibiotics. Plasmids do not directly play a role in protein synthesis (choice A) or DNA replication (choice B). Plasmids are not responsible for motility (choice D) as that function is usually carried out by flagella or other cellular structures.

Question 5 of 9

The decrease in blood clotting by heparin occurs at which general site of action?

Correct Answer: B

Rationale: The correct answer is B: Extracellular. Heparin works by inhibiting clotting factors in the blood outside of cells, preventing the formation of blood clots. It does not directly affect cellular, neuromuscular, or intracellular processes. By targeting extracellular factors, heparin effectively reduces the ability of blood to clot, making it an effective anticoagulant. The other choices are incorrect because heparin does not act on cellular, neuromuscular, or intracellular sites in the body to decrease blood clotting.

Question 6 of 9

Which of the following bacteria is known for causing urinary tract infections?

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. E. coli is the most common bacteria responsible for causing urinary tract infections due to its ability to adhere to the urinary tract lining and multiply rapidly. It is a normal inhabitant of the gut and can enter the urinary tract through improper hygiene practices. Salmonella enterica and Neisseria gonorrhoeae are typically associated with other infections like food poisoning and gonorrhea, respectively. Mycobacterium tuberculosis causes tuberculosis, not urinary tract infections. Thus, based on its prevalence and pathogenicity in the urinary tract, E. coli is the correct choice.

Question 7 of 9

Sputum smears of a patient with chronic pulmonary disease were stained by Ziehl-Neelsen method and analyzed in the bacteriological laboratory. Microscopy revealed red bacillus. What property of tuberculosis myobacteria was found?

Correct Answer: A

Rationale: The correct answer is A: Acid resistance. Tuberculosis mycobacteria have a unique cell wall structure that makes them resistant to acid-fast staining methods like Ziehl-Neelsen. This is due to the high lipid content of their cell walls, specifically mycolic acids, which prevent the penetration of stains and acids. The red bacilli observed in the smear indicate that the mycobacteria retained the primary stain (carbolfuchsin) after acid-alcohol decolorization, confirming their acid resistance. Summary: - Choice A: Acid resistance is correct due to the unique cell wall structure of tuberculosis mycobacteria. - Choice B: Alkali resistance is incorrect as mycobacteria are not known for being resistant to alkalis. - Choice C: Alcohol resistance is incorrect as mycobacteria are not specifically resistant to alcohols. - Choice D: Encapsulation is incorrect as mycobacteria are not encapsulated bacteria.

Question 8 of 9

As per their chemical structure the endotoxins are:

Correct Answer: A

Rationale: The correct answer is A: Lipopolysaccharides. Endotoxins are a type of bacterial toxin found in the outer membrane of Gram-negative bacteria. They consist of lipid A, which is a lipid component, and polysaccharides, making them lipopolysaccharides. Lipoproteins (choice B) and glycoproteins (choice C) do not accurately describe the chemical structure of endotoxins. "None of the above" (choice D) is incorrect as lipopolysaccharides are indeed the chemical structure of endotoxins.

Question 9 of 9

A 5-year-old child presents with a sore throat and a grayish pseudomembrane in the throat. Microscopic examination of a smear revealed dark blue-staining rods with polar granules. What is the causative agent?

Correct Answer: A

Rationale: The correct answer is A: Corynebacterium diphtheriae. This bacterium causes diphtheria, characterized by a grayish pseudomembrane in the throat. The dark blue-staining rods with polar granules seen in microscopic examination are known as metachromatic granules, which are specific to Corynebacterium diphtheriae. Streptococcus pyogenes (choice B) causes strep throat but does not produce pseudomembranes. Haemophilus influenzae (choice C) can cause respiratory infections but does not typically present with a pseudomembrane. Neisseria gonorrhoeae (choice D) is a sexually transmitted bacterium and is not associated with the symptoms described.

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