Correct Answer: B

Rationale: The correct answer is B: Conjugation. Conjugation involves the transfer of genetic material from a donor cell to a recipient cell through direct cell-to-cell contact, typically mediated by a conjugative plasmid. During conjugation, a conjugative pilus forms a bridge between the donor and recipient cells, allowing the transfer of genetic material. A: Replication is the process of copying DNA to produce two identical DNA molecules. It does not involve the transfer of genetic material between cells. C: Transformation is the uptake and incorporation of foreign DNA from the environment by a bacterial cell. It does not require direct cell-to-cell contact. D: Transduction is the transfer of genetic material from one bacterium to another by a bacteriophage vector. It does not involve direct cell-to-cell contact between the donor and recipient cells.

Correct Answer: A

Rationale: The correct answer is A: Corynebacterium diphtheriae. This bacterium causes diphtheria, characterized by a grayish pseudomembrane in the throat. The dark blue-staining rods with polar granules seen in microscopic examination are known as metachromatic granules, which are specific to Corynebacterium diphtheriae. Streptococcus pyogenes (choice B) causes strep throat but does not produce pseudomembranes. Haemophilus influenzae (choice C) can cause respiratory infections but does not typically present with a pseudomembrane. Neisseria gonorrhoeae (choice D) is a sexually transmitted bacterium and is not associated with the symptoms described.

Correct Answer: A

Rationale: The correct answer is A: Streptococcus pneumoniae. This bacterium appears as purple-stained diplococci with pointed ends on Gram staining. S. pneumoniae is a common cause of pneumonia. It is encapsulated and has a lancet-shaped appearance, which is characteristic of the pointed ends seen in the staining. Staphylococcus aureus (B) typically appears as clusters of gram-positive cocci, not diplococci. Klebsiella pneumoniae (C) is a gram-negative rod, not a diplococci. Neisseria meningitidis (D) is a gram-negative diplococci but typically lacks the pointed ends seen in the staining of S. pneumoniae.

Correct Answer: A

Rationale: The correct answer is A: Vibrio cholerae. 1. Vibrio cholerae is a Gram-negative, comma-shaped bacterium known to cause cholera. 2. Shigella dysenteriae causes dysentery, not cholera. 3. Salmonella typhi causes typhoid fever, not cholera. 4. Campylobacter jejuni causes gastroenteritis, not cholera. Therefore, based on the symptoms presented and the characteristics of the bacterium, Vibrio cholerae is the most likely microorganism.

Correct Answer: C

Rationale: The correct answer is C: mesophiles. Mesophiles are microorganisms that thrive in moderate temperatures, typically between 25°C and 40°C. This temperature range is ideal for their growth and reproduction. Thermophiles (A) prefer high temperatures, psychrophiles (B) thrive in cold temperatures, and psychrotrophs (D) can grow at refrigeration temperatures but have an optimal range above 20°C. Therefore, the mesophiles are the best fit for organisms that show optimal growth in moderate temperatures.