ATI RN
microbiology a systems approach test bank Questions
Question 1 of 9
The food preparation process in which not all microorganisms are killed, but the number of microbes capable of spoiling the food or causing disease is reduced, is called
Correct Answer: B
Rationale: The correct answer is B: pasteurization. This process involves heating food to a specific temperature to kill harmful bacteria while preserving the flavor and quality of the food. Sanitation (A) refers to maintaining cleanliness to prevent contamination. Disinfection (C) involves killing most, but not all, microbes on surfaces. Purification (D) typically refers to the process of removing impurities, not specifically targeting microbes. In this case, pasteurization best describes the process of reducing harmful microbes in food without completely eliminating all microorganisms.
Question 2 of 9
The role of pili in bacteria is:
Correct Answer: C
Rationale: The correct answer is C: adhesion to surfaces and conjugation. Pili are hair-like structures on the surface of bacteria that help them adhere to surfaces and facilitate the transfer of genetic material during conjugation. This is essential for bacterial survival and reproduction. Choice A is incorrect because protection from desiccation is typically associated with other structures like capsules. Choice B is incorrect as motility in bacteria is usually facilitated by flagella. Choice D is incorrect as spore formation involves the formation of specialized structures, not pili.
Question 3 of 9
A 40 year old man noticed a reddening and an edema of skin in the area of his neck that later developed into a small abscess. The incised focus is dense, yellowish-green. The pus contains white granules. Histological examination revealed drusen of a fungus, plasmatic and xanthome cells, macrophages. What type of mycosis is the most probable?
Correct Answer: D
Rationale: The correct answer is D: Sporotrichosis. Sporotrichosis is caused by the fungus Sporothrix schenckii, which commonly presents as a subcutaneous infection. In the given case, the presence of a small abscess with yellowish-green pus containing white granules is indicative of sporotrichosis. The histological examination showing drusen of a fungus, plasmatic and xanthome cells, and macrophages further supports the diagnosis of sporotrichosis. Choice A: Actinomycosis is a bacterial infection caused by Actinomyces species, not a fungal infection. Choice B: Aspergillosis is caused by Aspergillus species and typically presents as a pulmonary infection, not a subcutaneous abscess as described in the case. Choice C: Candidosis is caused by Candida species and usually presents as mucocutaneous infections, not a subcutaneous abscess with the
Question 4 of 9
A wound infection culture grew Gram-positive cocci in clusters. The bacteria were catalase-positive and coagulase-negative. What is the likely microorganism?
Correct Answer: A
Rationale: The correct answer is A: Staphylococcus epidermidis. The presence of Gram-positive cocci in clusters, along with being catalase-positive and coagulase-negative, is indicative of Staphylococcus species. Staphylococcus epidermidis is commonly found on the skin, making it likely to cause wound infections. Summary: B: Staphylococcus aureus is catalase-positive and coagulase-positive. C: Streptococcus pyogenes is catalase-negative. D: Enterococcus faecalis is catalase-negative.
Question 5 of 9
What are actinomycotic granules:
Correct Answer: C
Rationale: Step 1: Actinomycotic granules are colonies of actinomycetes located in purulent secretions. Step 2: Actinomycosis is a disease caused by actinomycetes forming these granules. Step 3: Actinomycotic granules are typically found in tissue exudates or pus. Step 4: Therefore, choice C is correct as it accurately describes actinomycotic granules. Summary: Choice A is incorrect because actinomycotic granules are not a form of actinomycosis itself, but a characteristic of the disease. Choice B is incorrect as actinomycotic granules are not an additional outer membrane but rather colonies of actinomycetes. Choice D is incorrect as actinomycotic granules are not intracellular inclusions but extracellular colonies found in purulent secretions.
Question 6 of 9
During the staining of sputum smear of a patient with suspected croupous pneumonia the following reactives and stainers were used: gentian violet solution, Lugol's solution, 960 spiritus and water fuchsin. What method of staining is used in this case?
Correct Answer: B
Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.
Question 7 of 9
Which structure in bacteria allows them to exchange genetic material during conjugation?
Correct Answer: A
Rationale: Pili in bacteria are responsible for conjugation, allowing the transfer of genetic material between cells. They form a bridge between donor and recipient cells, facilitating the exchange of DNA. Flagella are for movement, capsules for protection, and ribosomes for protein synthesis. Pili is the only structure directly involved in genetic material exchange.
Question 8 of 9
A specimen stained by Ozheshko method contains rod-like microorganisms stained blue with round terminal components stained red. What are these components called?
Correct Answer: A
Rationale: The correct answer is A: Spores. In the Ozheshko staining method, spores appear blue with red terminal components. Spores are reproductive structures produced by some bacteria, fungi, and protozoa. Cilia are short, hair-like structures used for movement. Flagella are long, whip-like structures also used for movement. Capsules are outer protective layers surrounding some bacteria. In this case, the description of rod-like microorganisms with blue staining and red terminal components aligns with the characteristics of spores, making it the correct answer.
Question 9 of 9
Infections of the CNS can be caused by:
Correct Answer: D
Rationale: The correct answer is D because all three choices, Clostridium botulinum, Morbillivirus morbillorum, and Clostridium tetani, can cause infections of the CNS. Clostridium botulinum can lead to botulism, affecting the nervous system. Morbillivirus morbillorum can cause measles, which can lead to encephalitis. Clostridium tetani can cause tetanus, affecting the central nervous system. Therefore, all three choices can cause infections of the CNS. Choices A, B, and C are incorrect individually because each pathogen can cause CNS infections.