ATI RN
microbiology chapter 13 test bank Questions
Question 1 of 5
The cleaning of glassware and tableware in restaurants falls into the category of
Correct Answer: D
Rationale: The correct answer is D: sanitization. Sanitization is the process of reducing the number of microorganisms on surfaces to a safe level. In restaurants, cleaning glassware and tableware aims to eliminate harmful bacteria to prevent foodborne illnesses. Degermation (A) focuses on removing germs from a specific area, sterilization (B) eliminates all microorganisms, and disinfection (C) reduces the number of pathogens but may not completely eliminate them, making them less effective choices for this context.
Question 2 of 5
A 10 year old child was subjected to Mantoux test (with tuberculin). 48 hours later a papule up to 8 mm in diameter appeared on the site of tuberculin injection. What type of hyperesponsiveness reaction has developed after tuberculin injection?
Correct Answer: A
Rationale: The correct answer is A: Hyperresponsiveness reaction type IV. This type of reaction, also known as delayed-type hypersensitivity, is characterized by the activation of T lymphocytes and macrophages, leading to inflammation at the site of antigen exposure. In the case of the Mantoux test, the appearance of a papule 48 hours later indicates a delayed hypersensitivity reaction mediated by T cells. Choice B: Reaction of Arthus phenomenon type is incorrect as this type of reaction involves immune complexes forming in the blood vessels leading to tissue damage, which is not the mechanism seen in the Mantoux test reaction. Choice C: Reaction of serum sickness type is incorrect as this type of reaction involves the formation of immune complexes in the blood leading to systemic symptoms like fever, rash, and joint pain, which is not typically seen in the Mantoux test reaction. Choice D: Atopic reaction is incorrect as atopic reactions involve IgE antibodies and mast cell degranulation, which are not the mechanisms
Question 3 of 5
During the skill-building session in microbiology the students need to stain the prepared and fixed sputum smears obtained from a tuberculosis patient. What staining technique should be used in this case?
Correct Answer: C
Rationale: The correct staining technique for staining sputum smears from a tuberculosis patient is Ziehl-Neelsen staining. This technique uses a carbol fuchsin stain that specifically stains acid-fast organisms like Mycobacterium tuberculosis. The steps involved include staining with carbol fuchsin, decolorizing with acid-alcohol, counterstaining with methylene blue, and then examining under a microscope. This staining method is crucial for detecting the acid-fast bacilli characteristic of tuberculosis. Explanation for incorrect choices: A: Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative, not suitable for acid-fast organisms like Mycobacterium tuberculosis. B: Giemsa staining is used for detecting parasites and certain types of white blood cells, not specific for acid-fast bacteria. D: Burry staining is not a recognized staining technique in microbiology for staining acid-fast organisms.
Question 4 of 5
On a certain territory mass death of rodents was registered. It was suspected that their death might have been caused by plague. What serological reaction should be applied for quick identification of antigen of the causative agent of this epizooty?
Correct Answer: B
Rationale: The correct answer is B: Agglutination. During a suspected plague outbreak, agglutination testing is used to quickly identify the antigen of the causative agent. In this test, specific antibodies are added to a sample containing the suspected antigen. If the antigen is present, it will clump together (agglutinate) with the antibodies, indicating a positive result. This method is rapid and efficient for identifying the presence of the plague-causing agent. Choice A: Precipitation involves the formation of a visible precipitate when antigen and antibody react, but it is not as quick or specific as agglutination for identifying the antigen in this scenario. Choice C: Passive hemagglutination is a variation of agglutination that uses red blood cells as carriers for antigens or antibodies. While it can be useful in certain situations, it is not typically the first choice for rapid identification of plague antigen. Choice D: Complement binding is a different type of
Question 5 of 5
For cultivation of Brucella, pure cultures should be incubated in CO2 enriched atmosphere. What type of breathing is typical for Brucella?
Correct Answer: A
Rationale: The correct answer is A: Capnophilic. Brucella requires a CO2 enriched atmosphere for cultivation, making it capnophilic. This is because Brucella is a microaerophilic bacterium that thrives in low oxygen conditions and elevated CO2 levels. Choice B (Facultative anaerobic) and D (Obligate aerobic) are incorrect as Brucella does not grow well under aerobic conditions. Choice C (Obligate anaerobic) is also incorrect as Brucella can grow in the presence of oxygen, although it prefers low oxygen environments.