The basic requirements of PCR reaction include:

Correct Answer: D

Rationale: The correct answer is D because all the options are essential for a PCR reaction. A: DNA segment is required as the template for amplification. B: Two oligonucleotide primers are needed to initiate DNA replication. C: Heat stable DNA polymerase (not just heat stable DNA) is crucial for withstanding the high temperatures during PCR. In summary, without any of these components, the PCR reaction cannot proceed effectively.

Correct Answer: D

Rationale: The correct answer is D because all measures mentioned are important in preventing hospital-acquired infections. Isolating the source helps prevent the spread of infections. Isolating at-risk patients reduces their exposure. Increasing patient resistance to infections through proper hygiene and immunizations minimizes susceptibility. Each measure plays a crucial role in infection prevention, making all options correct.

Correct Answer: D

Rationale: The correct answer is D: LT enterotoxin. This toxin is specific for Enterotoxigenic E. coli (ETEC) and is responsible for causing diarrhea by increasing cyclic AMP levels in intestinal cells. TSST-1 (choice A) is produced by Staphylococcus aureus, not ETEC. Shiga-like toxin (choice B) is produced by Shigella and EHEC, not ETEC. Erythrogenic toxin (choice C) is produced by Streptococcus pyogenes, not ETEC. Therefore, the LT enterotoxin is the specific toxin associated with ETEC.

Correct Answer: A

Rationale: The correct answer is A: Syphilitic granulomas (gummas). In the tertiary stage of syphilis, gummas, which are soft, tumor-like growths, can develop in various organs such as the skin, bones, liver, and brain. This stage typically occurs years after the initial infection and can lead to serious complications if left untreated. Purulent exudation from the urethra (B) is not specific to syphilis and may indicate other infections. Non-tender chancre (C) is characteristic of the primary stage of syphilis, not the tertiary stage. Diffuse maculopapular rash (D) is more commonly seen in the secondary stage of syphilis.

Correct Answer: C

Rationale: The correct answer is C. Negri bodies are not associated with measles but with rabies. Step 1: Measles is a childhood infection with a rash (A). Step 2: Koplik spots are small white spots on a red base found inside the mouth and are characteristic of measles (B). Step 3: Negri bodies are eosinophilic cytoplasmic inclusions found in neurons infected with rabies virus, not measles (C). Step 4: Prophylaxis for measles is through the MMR vaccine, which is a live attenuated vaccine (D). Therefore, choice C is incorrect as it does not pertain to measles.