The ability of Clostridium tetani to produce exotoxins is encoded by?

Correct Answer: C

Rationale: The correct answer is C: genes with plasmid location. Clostridium tetani produces exotoxins through genes located on plasmids. Plasmids are extrachromosomal genetic elements that carry additional genes. These genes encode for the production of exotoxins in Clostridium tetani. Choices A, B, and D are incorrect because Clostridium tetani does produce exotoxins, it does not have only endotoxin activity, and the genes responsible for exotoxin production are located on plasmids, not solely on the chromosome or both chromosome and plasmid.

Correct Answer: B

Rationale: The correct answer is B: Plasmids. Plasmids are extrachromosomal DNA molecules found in bacteria that can replicate independently of the bacterial chromosome. They carry additional genetic information, such as antibiotic resistance genes or virulence factors. Mesosomes (choice A) are not involved in carrying genetic information; they are infoldings of the cell membrane. Ribosomes (choice C) are cellular organelles responsible for protein synthesis, not carriers of genetic information. Choice D is incorrect as plasmids are indeed extrachromosomal carriers of genetic information in bacteria.

Correct Answer: D

Rationale: The correct answer is D: Loefler. This staining method is used for identifying Corynebacterium diphtheriae, the causative agent of diphtheria. It involves using methylene blue and basic fuchsin to stain the bacteria. The yellow bacilli with blue grains at their ends indicate the characteristic appearance of C. diphtheriae when stained using the Loefler method. Choice A: Ziehl-Nielsen is used for acid-fast bacteria like Mycobacterium tuberculosis. Choice B: Kozlovsky is not a standard staining method in microbiology. Choice C: Romanovsky is used for blood smears to identify malaria parasites.

Correct Answer: B

Rationale: The correct answer is B: EBV. Osteomyelitis is primarily caused by bacteria, not viruses like Epstein-Barr virus (EBV). The main pathogens involved in osteomyelitis are typically Staphylococcus aureus, including Methicillin-resistant Staphylococcus aureus (MRSA), Haemophilus influenzae, and Pseudomonas aeruginosa. EBV is not a causative agent of osteomyelitis. It is crucial to differentiate between bacterial and viral etiologies in infectious diseases to guide appropriate treatment.

Correct Answer: C

Rationale: The correct answer is C: Up to 3 months. Hepatitis B has an average incubation period of 60-90 days, which aligns with the timeframe of up to 3 months. This period refers to the time between exposure to the virus and the onset of symptoms. One week to 10 days (choice A) is too short for hepatitis B, as it typically takes longer to manifest. One month to 45 days (choice B) is also shorter than the average incubation period for hepatitis B. Up to 6 months (choice D) is too long for hepatitis B, as the virus typically shows symptoms within 3 months.

Correct Answer: B

Rationale: The Ziehl-Neelsen stain is specifically designed to detect acid-fast bacteria like Mycobacterium tuberculosis. It utilizes a carbol fuchsin dye that binds to the high lipid content in the cell wall of acid-fast bacteria, making them appear red under a microscope. This staining method is crucial for the diagnosis of tuberculosis and leprosy. Gram-positive bacteria (Choice A) are not detected by this stain since they have a different cell wall structure. Capsulated bacteria (Choice C) and fungal spores (Choice D) also have distinct characteristics that are not targeted by the Ziehl-Neelsen stain.

Correct Answer: C

Rationale: The correct answer is C (100°C) because the Koch apparatus is used for sterilization, and the standard temperature for sterilization is 100°C. At this temperature, most microorganisms are killed, making it an effective method for sterilization. Choice A (90°C), B (95°C), and D (110°C) are incorrect because they are not commonly used temperatures for sterilization purposes. Choosing a temperature lower than 100°C may not effectively kill all microorganisms, while choosing a temperature higher than 100°C may lead to unnecessary energy consumption without providing additional sterilization benefits.

Correct Answer: A

Rationale: Acyclovir is the correct answer because it is an antiviral drug used to treat herpes simplex and varicella-zoster viruses, not influenza. Tamiflu, Amantadine, and Relenza are used to treat influenza by inhibiting viral replication or easing symptoms. Acyclovir's mechanism of action targets different viruses, making it ineffective for treating influenza.

Correct Answer: A

Rationale: The correct answer is A: Metronidazole. Parodontosis is a condition caused by bacterial infection, not protozoal infection. Metronidazole is an effective antibiotic commonly used to treat bacterial infections in the oral cavity, including those associated with parodontosis. Levamisole is an anthelmintic drug used to treat parasitic worm infections, not suitable for parodontosis. Griseofulvin is an antifungal medication used for fungal infections, not indicated for parodontosis. Mykoseptin is not a recognized drug and is likely a distractor. Therefore, based on the bacterial nature of parodontosis, Metronidazole is the appropriate choice.