Select incorrect mechanism for the development of autoimmune disorder.

Correct Answer: A

Rationale: The correct answer is A. A second exposure to an allergen does not lead to the development of autoimmune disorders. The key mechanisms involved in autoimmune disorders are self-tolerance breakdown and immune system attacking self-antigens. Explanation: 1. B: Cross-reaction of antibodies formed against foreign antigens with self-antigens is a possible mechanism for autoimmune disorders. 2. C: Exposure of previously 'hidden' self-antigens to the adaptive immune system can trigger autoimmune responses. 3. D: Mutation followed by the appearance of membrane proteins not previously present can lead to autoimmune disorders, as the immune system may recognize these proteins as foreign. Summary: Choice A is incorrect because a second exposure to an allergen does not cause the immune system to target self-antigens, which is the hallmark of autoimmune disorders. Choices B, C, and D are more plausible mechanisms for the development of autoimmune disorders.

Correct Answer: C

Rationale: The correct answer is C: 5' cohesive ends. Sacl restriction endonuclease cuts DNA at specific recognition sites, leaving single-stranded overhangs called cohesive ends. In this case, Sacl creates 5' overhangs, resulting in fragments with 5' cohesive ends. Blunt ends (option A) have no overhangs, making them incorrect. Options B and D are incorrect as they do not align with the specific characteristic of Sacl restriction endonuclease's cleavage pattern.

Correct Answer: A

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Correct Answer: C

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Correct Answer: A

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.