Sanitary bacteriological research on water by the membrane filter method revealed two red colonies on a membrane filter (Endo agar) through which 500 ml of analyzed water were passed. Calculate the coli index and coli titer of the analyzed water:

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Question 1 of 9

Sanitary bacteriological research on water by the membrane filter method revealed two red colonies on a membrane filter (Endo agar) through which 500 ml of analyzed water were passed. Calculate the coli index and coli titer of the analyzed water:

Correct Answer: A

Rationale: The correct answer is A: 4 and 250. Coli index is calculated by dividing the number of red colonies by the volume of water analyzed, which gives 2 red colonies / 500 ml = 4. Coli titer is the reciprocal of the coli index, so it is 1 / 4 = 250. This indicates the concentration of coliform bacteria in the water sample. Choices B, C, and D are incorrect as they do not follow the correct calculation steps for determining the coli index and coli titer. B has the correct coli index but incorrect coli titer. C and D have the values reversed, leading to incorrect results.

Question 2 of 9

Which bacteria are Gram-negative cocci?

Correct Answer: A

Rationale: The correct answer is A: Neisseria species. Gram staining differentiates bacteria based on cell wall composition. Gram-negative bacteria like Neisseria have a thin peptidoglycan layer and an outer membrane, making them appear pink under the microscope. Streptococcus species (B) are Gram-positive cocci with a thick peptidoglycan layer, appearing purple. Bacillus (C) and Clostridium (D) species are Gram-positive rods, not Gram-negative cocci. Thus, the correct answer is A because Neisseria species are the only Gram-negative cocci among the choices provided.

Question 3 of 9

All statements of viral neutralization reaction are true except:

Correct Answer: A

Rationale: Rationale: A: Incorrect - The complement system is not directly involved in the viral neutralization reaction. B: Correct - Specific antibodies neutralize viruses by blocking their ability to infect cells. C: Incorrect - Antibodies are titrated, but only one serum sample is required for neutralization testing. D: Incorrect - Viral neutralization is primarily used to measure antibody response, not to identify newly isolated viruses.

Question 4 of 9

Extrachromosomal carriers of the genetic information in bacteria are:

Correct Answer: B

Rationale: The correct answer is B: Plasmids. Plasmids are extrachromosomal DNA molecules found in bacteria that can replicate independently of the bacterial chromosome. They carry additional genetic information, such as antibiotic resistance genes or virulence factors. Mesosomes (choice A) are not involved in carrying genetic information; they are infoldings of the cell membrane. Ribosomes (choice C) are cellular organelles responsible for protein synthesis, not carriers of genetic information. Choice D is incorrect as plasmids are indeed extrachromosomal carriers of genetic information in bacteria.

Question 5 of 9

Which of the following bacteria are capable of forming spores?

Correct Answer: B

Rationale: The correct answer is B, Clostridium botulinum. This bacterium is capable of forming spores, which are dormant, resistant structures that help bacteria survive harsh conditions. Staphylococcus aureus (A), Escherichia coli (C), and Neisseria gonorrhoeae (D) do not form spores. Staphylococcus aureus is a gram-positive bacterium that does not produce spores. Escherichia coli is a gram-negative bacterium that also does not form spores. Neisseria gonorrhoeae is a gram-negative bacterium and does not have the ability to form spores. Therefore, the only bacteria among the options that can form spores is Clostridium botulinum.

Question 6 of 9

A patient's preliminary diagnosis is toxoplasmosis. What material was used for diagnostics of this disease?

Correct Answer: A

Rationale: The correct answer is A: Blood. Toxoplasmosis is diagnosed through serological tests that detect specific antibodies in the blood. These antibodies indicate exposure to the Toxoplasma gondii parasite. Feces, urine, and duodenal contents are not typically used for diagnosing toxoplasmosis as the parasite is not commonly found in these samples. Blood is the primary material used as it provides the necessary antibodies for accurate diagnosis.

Question 7 of 9

Humoral factors of the natural resistance are:

Correct Answer: B

Rationale: The correct answer is B because lysozyme, lactoferrin, interferon, and complement are all humoral factors involved in natural resistance. Lysozyme and lactoferrin are antimicrobial proteins that help defend against pathogens. Interferon is a signaling protein that inhibits viral replication, while complement is a group of proteins that enhance the immune response by promoting inflammation and opsonization. Choices A, C, and D are incorrect: - Choice A mentions only macrophages, which are not humoral factors but rather phagocytic cells. - Choice C lists immune cells involved in adaptive immunity (B and T lymphocytes, NK cells) rather than humoral factors of natural resistance. - Choice D includes immunoglobulins, which are part of adaptive immunity, not natural resistance.

Question 8 of 9

A patient suffers from pulmonary tuberculosis. During treatment neuritis of visual nerve arose. What drug has caused this by-effect?

Correct Answer: B

Rationale: The correct answer is B: Ethambutol. Ethambutol is known to cause optic neuritis, affecting the visual nerve. It is a well-documented side effect of this drug. Isoniazid (Choice A) is associated with peripheral neuropathy, not optic neuritis. Kanamycin (Choice C) is known for its side effects on the inner ear, causing hearing loss. Rifampicin (Choice D) is not typically associated with optic neuritis but can cause other adverse effects like hepatitis. By process of elimination and knowledge of drug side effects, Ethambutol is the most likely culprit.

Question 9 of 9

Which of the following(s) is/are the determinants of antigenicity?

Correct Answer: D

Rationale: The correct answer is D, as all options A, B, and C are determinants of antigenicity. A: Chemical nature influences how the immune system recognizes antigens. B: The size of the antigen affects its ability to be recognized by immune cells. C: Antigens of foreign origin are more likely to trigger an immune response. Therefore, all of these factors together play a crucial role in determining antigenicity.

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