Sanitary bacteriological research on water by the membrane filter method revealed two red colonies on a membrane filter (Endo agar) through which 500 ml of analyzed water were passed. Calculate the coli index and coli titer of the analyzed water:

Correct Answer: A

Rationale: The correct answer is A: 4 and 250. Coli index is calculated by dividing the number of red colonies by the volume of water analyzed, which gives 2 red colonies / 500 ml = 4. Coli titer is the reciprocal of the coli index, so it is 1 / 4 = 250. This indicates the concentration of coliform bacteria in the water sample. Choices B, C, and D are incorrect as they do not follow the correct calculation steps for determining the coli index and coli titer. B has the correct coli index but incorrect coli titer. C and D have the values reversed, leading to incorrect results.

Correct Answer: A

Rationale: The correct answer is A: Acid-fast stain. This stain is used for diagnosing tuberculosis due to the unique cell wall composition of Mycobacterium tuberculosis, which resists decolorization by acid-alcohol. This allows the bacteria to retain the primary stain (carbol fuchsin) and appear red under a microscope. Gram stain (B) is not suitable for Mycobacterium tuberculosis as it is gram-positive but does not retain the crystal violet-iodine complex. Negative stain (C) is not used for bacterial identification. Simple stain (D) does not differentiate acid-fast bacteria like Mycobacterium tuberculosis.

Correct Answer: B

Rationale: The correct answer is B: Clostridium tetani. This bacterium produces tetanospasmin toxin, causing tetanus. Clostridium botulinum (choice A) produces botulinum toxin, leading to botulism. Bacillus anthracis (choice C) causes anthrax, not tetanus. Staphylococcus aureus (choice D) can cause various infections, but not tetanus. Therefore, only Clostridium tetani is the causative agent of tetanus.

Correct Answer: A

Rationale: The correct answer is A: Neisseria species. Gram staining differentiates bacteria based on cell wall composition. Gram-negative bacteria like Neisseria have a thin peptidoglycan layer and an outer membrane, making them appear pink under the microscope. Streptococcus species (B) are Gram-positive cocci with a thick peptidoglycan layer, appearing purple. Bacillus (C) and Clostridium (D) species are Gram-positive rods, not Gram-negative cocci. Thus, the correct answer is A because Neisseria species are the only Gram-negative cocci among the choices provided.

Correct Answer: A

Rationale: The correct answer is A: Anaerobic. Pulpitis is an inflammatory condition of the dental pulp, often caused by bacterial infection. Anaerobic bacteria are commonly associated with dental infections, making it likely to find them in the inoculated culture medium. Anaerobic bacteria thrive in the absence of oxygen, which is typical of the dental environment. Acid-resistant (B) and acidophilic (C) microorganisms are not specifically linked to pulpitis. Haemolytic (D) microorganisms typically lyse red blood cells, which is not a characteristic feature of bacteria causing pulpitis. Therefore, the most likely microorganisms to be found in the culture medium would be anaerobic bacteria.

Correct Answer: B

Rationale: The correct answer is B because lysozyme, lactoferrin, interferon, and complement are all humoral factors involved in natural resistance. Lysozyme and lactoferrin are antimicrobial proteins that help defend against pathogens. Interferon is a signaling protein that inhibits viral replication, while complement is a group of proteins that enhance the immune response by promoting inflammation and opsonization. Choices A, C, and D are incorrect: - Choice A mentions only macrophages, which are not humoral factors but rather phagocytic cells. - Choice C lists immune cells involved in adaptive immunity (B and T lymphocytes, NK cells) rather than humoral factors of natural resistance. - Choice D includes immunoglobulins, which are part of adaptive immunity, not natural resistance.

Correct Answer: A

Rationale: The correct answer is A: Helicobacter pylori. Rationale: 1. Gram-negative spiral-shaped microorganisms match the morphology of Helicobacter pylori. 2. Positive urease activity is a characteristic feature of Helicjsonacter pylori. 3. Helicobacter pylori is specifically associated with gastric ulcers and is a common pathogen in the gastrointestinal tract. Summary: Choice B, Campilobacter jejuni, is a gram-negative curved bacterium but is not typically associated with gastric ulcers or urease activity. Choice C, Treponema pallidum, is a spirochete bacterium causing syphilis, which is not related to gastric ulcers. Choice D, Spirilla minor, is a generic term for spiral-shaped bacteria and is not a specific pathogen associated with gastric ulcers or urease activity.

Correct Answer: A

Rationale: The correct answer is A: Air. In transmissive infections, the vector refers to the means by which the infectious agent is transmitted from one host to another. Air can carry pathogens such as viruses and bacteria, leading to respiratory infections like the flu or tuberculosis. Airborne transmission occurs when droplets containing the pathogens are inhaled. Objects (B), medical devices (C), and water (D) can also transmit infections, but they are not specifically related to transmissive infections. Objects and medical devices can harbor pathogens, while waterborne infections are transmitted through contaminated water sources. However, in the context of transmissive infections, the primary vector is the air due to its ability to carry infectious agents over distances.

Correct Answer: B

Rationale: The correct answer is B: Yersinia enterocolitica. Y. enterocolitica is known to cause gastroenteritis and diarrhea in humans. It is often associated with contaminated food or water. Bordetella pertussis causes whooping cough, not diarrhea. Corynebacterium diphtheria causes respiratory diphtheria. Neisseria meningitidis is associated with meningitis and septicemia, not diarrhea. Therefore, based on the patient's symptoms and history, Y. enterocolitica is the most likely cause of the diarrhea in this case.