Sanitary and bacteriological examination of air in drug-store premises revealed increased content of sanitary representative microorganisms. What microorganisms are these?

Correct Answer: A

Rationale: The correct answer is A (Golden staphylococcus and hemolytic streptococcus) because these microorganisms are commonly found in the air of contaminated environments like drug-store premises. Golden staphylococcus and hemolytic streptococcus are pathogenic bacteria associated with infections in humans, indicating poor sanitation. The other choices are incorrect because: B: Diphtheria and tuberculosis bacilli are not commonly found in the air in drug-store premises. C: Colon and blue pus bacilli are not typical microorganisms associated with air contamination. D: Epidermal staphylococcus and sarcina are not likely to be present in high amounts in the air of drug-store premises.

Correct Answer: A

Rationale: The correct answer is A: Serological. Serological testing involves analyzing blood samples for specific antibodies produced in response to the pertussis infection. This method can detect past infections by identifying the presence of antibodies against the pertussis bacteria in the blood. In this case, since the doctor suspects pertussis infection in both children, serological testing can confirm if they have been exposed to the bacteria. Summary: B: Biological testing does not specifically target pertussis antibodies in the blood. C: Bacteriological testing involves culturing bacteria from samples, which may not be as sensitive for retrospective diagnosis. D: Molecular biological testing focuses on genetic material of the bacteria, which may not provide direct evidence of past infection.

Correct Answer: A

Rationale: The presence of flagella in a bacterial cell represents the H-antigen. Flagella are protein structures on the cell surface that play a role in bacterial motility. The H-antigen refers to the flagellar antigen, which is involved in immune responses and bacterial identification. Option A is correct because flagella are specifically associated with the H-antigen. Summarily, choices B and C are incorrect because the O-antigen and K-antigen are associated with lipopolysaccharide and capsule antigens, respectively, not flagella. Option D is incorrect as well because the correct association is with the H-antigen.

Correct Answer: B

Rationale: The correct answer is B: mutualism. In mutualism, both organisms benefit from the relationship. This is because they provide each other with resources or services that help both parties. In contrast, commensalism (A) is a relationship where one organism benefits while the other is neither helped nor harmed. Parasitism (C) is a relationship where one organism benefits at the expense of the other. Competition (D) is a relationship where both organisms are negatively affected as they compete for limited resources. Therefore, mutualism is the correct answer as it describes a mutually beneficial relationship between two organisms.

Correct Answer: C

Rationale: Step 1: S. aureus is known to produce hemolysins, leucocidins, and enterotoxin. Step 2: S. aureus colonies usually exhibit golden-yellow pigmentation with beta-hemolytic zones. Step 3: S. aureus has developed resistance to penicillin due to the production of beta-lactamase. Step 4: Therefore, statement C is not true as S. aureus is not always sensitive to penicillin. Summary: Choices A and B are correct statements about S. aureus, while choice C is incorrect due to the increasing penicillin resistance seen in S. aureus.

Correct Answer: D

Rationale: The correct answer is D: LT enterotoxin. This toxin is specific for Enterotoxigenic E. coli (ETEC) and is responsible for causing diarrhea by increasing cyclic AMP levels in intestinal cells. TSST-1 (choice A) is produced by Staphylococcus aureus, not ETEC. Shiga-like toxin (choice B) is produced by Shigella and EHEC, not ETEC. Erythrogenic toxin (choice C) is produced by Streptococcus pyogenes, not ETEC. Therefore, the LT enterotoxin is the specific toxin associated with ETEC.

Correct Answer: C

Rationale: The correct answer is C: fungi. Both penicillin and cephalosporin are produced by certain species of fungi. Penicillin is derived from the Penicillium fungi, while cephalosporin is produced by Cephalosporium fungi. Fungi have the ability to synthesize these antibiotics as part of their defense mechanisms against other microorganisms. Algae (A), slime molds (B), and protozoans (D) do not have the capability to produce antibiotics like penicillin and cephalosporin. Algae are mainly photosynthetic organisms, slime molds are protists that lack the ability to synthesize complex antibiotics, and protozoans are single-celled eukaryotic organisms that do not produce antibiotics like fungi do.

Correct Answer: D

Rationale: The correct answer is D: peroxisomes. Peroxisomes contain oxidative enzymes, such as catalase and peroxidase, that are capable of oxidizing toxic substances. These enzymes help detoxify harmful molecules by breaking them down into harmless products. Lysosomes (choices B and C) contain hydrolytic enzymes for breaking down cellular waste, not oxidative enzymes. Therefore, the correct choice is peroxisomes due to their specific function in detoxification through oxidation reactions.

Correct Answer: D

Rationale: The correct answer is D. Cytoplasmic inclusions in bacteria refer to substances like granules. These granules can be excess products of metabolism or reserve substances stored for future use. Therefore, all of the choices are correct as cytoplasmic inclusions can be any of these substances. A is correct as it directly refers to cytoplasmic inclusions. B is correct as some granules can be excess metabolic products. C is correct as certain granules can serve as reserve substances. Therefore, the correct answer is D, as it encompasses all the possible types of substances found as cytoplasmic inclusions in bacteria.