Routine investigation of microbiological sanitary condition of air in a hospital is performed once in 3 months. What microorganism is the sanitary indicator of air condition in an enclosed space?

Correct Answer: D

Rationale: The correct answer is D: P.aeruginosa. This bacterium is commonly found in water and soil, making its presence in indoor air an indicator of poor sanitation. Step 1: P.aeruginosa is an opportunistic pathogen known to cause infections in hospitals. Step 2: Its presence in the air indicates potential contamination and poor air quality. Step 3: Regular monitoring of P.aeruginosa levels can help assess the effectiveness of sanitation measures. Summary: A, B, and C are not suitable indicators of air sanitation in an enclosed space as they are not typically associated with environmental contamination or poor air quality.

Correct Answer: D

Rationale: The correct answer is D: P.aeruginosa. This bacterium is commonly found in water and soil, making its presence in indoor air an indicator of poor sanitation. Step 1: P.aeruginosa is an opportunistic pathogen known to cause infections in hospitals. Step 2: Its presence in the air indicates potential contamination and poor air quality. Step 3: Regular monitoring of P.aeruginosa levels can help assess the effectiveness of sanitation measures. Summary: A, B, and C are not suitable indicators of air sanitation in an enclosed space as they are not typically associated with environmental contamination or poor air quality.

Correct Answer: B

Rationale: The correct answer is B: acidic pH. The reproductive tract's acidic pH serves as a defense mechanism by creating an environment hostile to many pathogens, preventing microbial infection. The acidic pH helps to inhibit the growth and survival of harmful microorganisms. A: Alkaline pH would actually be more favorable for microbial growth as most pathogens thrive in neutral to alkaline pH environments. C: High sugar concentration can actually promote microbial growth as many pathogens feed on sugars. D: High body temperature may help in some cases, but it is not as effective as an acidic pH in preventing microbial infection in the reproductive tract.

Correct Answer: C

Rationale: The correct answer is C: Negative stain. Negative staining uses acidic dyes which are repelled by the bacterial cell wall components, highlighting bacteria with a large amount of peptidoglycan. Acid-fast stain (A) is used to identify Mycobacterium species with waxy cell walls. Gram stain (B) differentiates bacteria into Gram-positive and Gram-negative based on cell wall composition. Option D is a duplicate of option C and is incorrect.

Correct Answer: D

Rationale: The correct answer is D because all three bacteria listed (Staphylococcus aureus, Escherichia coli, Clostridium botulinum) can produce toxins leading to food poisoning. Staphylococcus aureus produces heat-stable toxins causing rapid onset of symptoms. Escherichia coli can produce toxins like Shiga toxin leading to severe foodborne illness. Clostridium botulinum produces neurotoxins causing botulism. Choice A, B, and C are incorrect because each bacteria can produce toxins that are harmful if ingested.

Correct Answer: A

Rationale: The correct answer is A: Toxin production test. Rationale: 1. Diphtheria is caused by a toxin produced by Corynebacterium diphtheriae. 2. To confirm if the carrier is dangerous, we need to test if they are producing the toxin. 3. The toxin production test directly assesses if the bacteria are producing the harmful toxin. 4. Positive results indicate the carrier is dangerous and can transmit the disease to others. Summary of other choices: B: Mouse neutralization test - Used to detect diphtheria antitoxin levels in blood, not to confirm if the carrier is dangerous. C: Immunofluorescence test - Used to detect specific antigens or antibodies, not directly related to confirming the carrier's danger. D: Complement fixation test - Measures immune response, not specific to toxin production confirmation.

Correct Answer: A

Rationale: The correct answer is A: T-lymphocytes. Cell-mediated immunity involves the activation of T-lymphocytes, which play a crucial role in recognizing and killing infected cells. T-lymphocytes include cytotoxic T cells, helper T cells, and regulatory T cells, all of which are involved in different aspects of cell-mediated immunity. Monocytes (choice B) are a type of white blood cell involved in phagocytosis but are not the main mediators of cell-mediated immunity. B-lymphocytes (choice C) are involved in humoral immunity, producing antibodies, not cell-mediated immunity. Choice D (A+B+C) is incorrect because cell-mediated immunity is mainly mediated by T-lymphocytes.

Correct Answer: B

Rationale: The correct answer is B because antigenic drift is not caused by the exchange of genetic information between animal and human influenza viruses. Antigenic drift is actually due to point mutations in the genes that encode for the surface proteins of the virus, leading to small changes in the antigens. These mutations can affect the hemagglutinin of both type A and B influenza viruses, making choices A and C correct. Choice D is incorrect because antigenic drift can occur in both type A and B influenza viruses, with type B being less prone to drift compared to type A.

Correct Answer: D

Rationale: The correct answer is D, Salmonella typhi, because it is a motile bacterium due to possessing flagella that aid in movement. Bacillus anthracis (A) is non-motile, Klebsiella pneumoniae (B) lacks flagella for motility, and Shigella flexneri (C) is non-motile as well. Salmonella typhi (D) is known for its flagella-driven motility, allowing it to move and spread within its host and environment. Flagella are essential for bacterial motility, enabling them to navigate and colonize different environments efficiently.