Patient with vomiting, dizziness, sensation of dubble vision, difficult swallowing was admitted to the hospital. Doctor suspects botulism. What diagnostic methods should be used for diagnosis approving?

Correct Answer: C

Rationale: The correct answer is C: Biological test, bacteriological. Botulism is caused by the toxin produced by Clostridium botulinum bacteria. To diagnose botulism, biological tests such as detecting the toxin in patient samples and bacteriological tests to identify the bacteria are crucial. These tests confirm the presence of the bacteria and its toxin, providing a definitive diagnosis. Choice A is incorrect as it does not specify any relevant diagnostic methods. Choice B is incorrect because mycological tests are not relevant for diagnosing botulism caused by a bacterial toxin. Choice D is incorrect as allergic tests and serological tests are not used for diagnosing botulism.

Correct Answer: A

Rationale: The correct answer is A: Clostridium tetani. Clostridium tetani is responsible for causing tetanus by producing a neurotoxin called tetanospasmin. This neurotoxin blocks inhibitory signals in the central nervous system, leading to muscle spasms and stiffness characteristic of tetanus. Clostridium perfringens (B) causes gas gangrene, Streptococcus pyogenes (C) causes strep throat, and Escherichia coli (D) can cause gastrointestinal infections but not tetanus. Therefore, based on the specific pathogen and its mechanism of action, Clostridium tetani is the correct answer.

Correct Answer: B

Rationale: Step-by-step rationale: 1. Fimbriae are hair-like structures on bacteria, not rope-like. 2. Fimbriae are smaller in diameter compared to flagella. 3. Fimbriae are used for adherence or conjugation, not flagella. Therefore, choice B is correct as it incorrectly describes fimbriae, while choices A, C, and D correctly characterize fimbriae.

Correct Answer: C

Rationale: The correct answer is C, Meat-peptone agar, as it provides a simple nutritive media for bacterial growth. This agar contains meat extract and peptone, which are good sources of nutrients for bacterial growth. Glucose broth (A) is a liquid medium with glucose but lacks other essential nutrients. Ascites (serum) agar (B) is a selective medium used for detecting pathogens in clinical samples. Blood agar (D) is a differential medium used to distinguish different types of bacteria based on hemolytic reactions. Meat-peptone agar is the simplest and most general-purpose medium among the options provided.

Correct Answer: C

Rationale: Rationale: 1. Candida is a fungus commonly isolated from clinical specimens. 2. Sabouraud agar is the most suitable medium for Candida due to its low pH and high dextrose content. 3. Low pH inhibits bacterial growth, while dextrose promotes fungal growth. 4. Zeisler media, Chocolate agar, and Muller-Hinton agar are not specific for fungal isolation. Summary: - Zeisler media is used for anaerobic bacteria. - Chocolate agar is used for fastidious organisms like Haemophilus. - Muller-Hinton agar is for antibiotic susceptibility testing, not fungal isolation.