N. Meningitidis grows on

Correct Answer: D

Rationale: The correct answer is D: Chocolate agar. Neisseria meningitidis is a fastidious organism that requires enriched media for growth. Chocolate agar contains heat-treated blood that provides essential nutrients for the growth of N. meningitidis. The other choices, A: Levine agar, B: Lowenstein-Jensen agar, and C: TCBS agar, do not provide the specific nutrients required for the growth of N. meningitidis. Therefore, only choice D, Chocolate agar, supports the growth of N. meningitidis due to its enriched composition.

Correct Answer: C

Rationale: Step 1: "Fried egg" colonies typically refer to colonies with a raised center and flattened periphery, resembling the appearance of a fried egg. Step 2: Actinomycetes are filamentous bacteria known to form "fried egg" colonies in nutrient medium. Step 3: Actinomycetes are commonly found in soil and are known to be phytopathogenic microorganisms. Step 4: Actinomycetes produce secondary metabolites with medicinal properties, making them a likely agent from a medicinal herb. Overall, the correct answer is C (Actinomycetes) as they fit the description provided in the question and are commonly associated with the given scenario. The other choices are incorrect as they do not exhibit the specific characteristics mentioned in the question or are not typically associated with forming "fried egg" colonies in nutrient medium.

Correct Answer: B

Rationale: The correct answer is B: H antigen. Flagellar antigen is typically referred to as the H antigen in bacteria. Flagella are the whip-like structures used for movement in bacteria, and the H antigen is specifically associated with the flagella. The other choices (A: D antigen, C: K antigen, D: F antigen) are not related to flagella in bacteria and are commonly associated with other types of antigens in bacterial classification.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Adenoviruses are DNA viruses, so choices B and C (ssRNA and DNA) are incorrect. 2. Adenoviruses have a middle-sized, naked icosahedral capsid, making choice A the correct description. 3. Choice D is incorrect as adenoviruses do have specific characteristics, making "None of the above" an inaccurate option. In summary, the correct answer A is supported by the fact that adenoviruses have a middle-sized, naked icosahedral capsid and contain double-stranded DNA. Choices B, C, and D are incorrect as they do not align with the characteristics of adenoviruses.

Correct Answer: C

Rationale: The correct answer is C because Rickettsia requires living cells for growth and replication. Chick embryos, cell cultures, and lab animals provide a suitable environment for Rickettsia isolation. Skin-allergy tests (A) and serological methods for proving antibodies (B) are not directly used for isolating Rickettsia as they do not support the growth of the bacteria. "None of the above" (D) is incorrect as option C involves the appropriate methods for isolating Rickettsia.

Correct Answer: B

Rationale: The correct answer is B because sterilization is the process of completely destroying all microorganisms, including resistant forms like spores, ensuring no viable organisms remain. This is crucial for ensuring complete elimination of potential pathogens. Choice A is incorrect because sterilization should eliminate all microorganisms, including spores. Choice C is incorrect as it describes disinfection, not sterilization. Choice D is incorrect because sterilization aims to eradicate all microorganisms, not leave any viable ones behind.

Correct Answer: B

Rationale: The correct answer is B: Enable bacterial adherence to surfaces. Bacterial capsules are a protective layer outside the cell wall that helps bacteria adhere to surfaces and evade host immune responses. This allows bacteria to colonize and infect host tissues. A: Enhancing bacterial motility is typically associated with flagella, not capsules. C: Bacterial DNA replication is not directly affected by capsules. D: Bacterial capsules do play a role in protecting bacteria from desiccation by providing a barrier against drying out. However, the primary function is enabling adherence to surfaces.

Correct Answer: B

Rationale: The correct answer is B: Contains single-stranded RNA with negative polarity. Measles virus is an enveloped virus with a single-stranded RNA genome that is negative-sense. This means it must first be transcribed into positive-sense RNA before it can be translated. Choice A is incorrect as the measles virus does have an outer envelope. Choice C is incorrect as measles virus belongs to the family Paramyxoviridae, not Togaviridae. Choice D is incorrect as measles virus can be grown on cell cultures, specifically on Vero cells.

Correct Answer: A

Rationale: The correct answer is A: Neutralization. In this scenario, the antibotulinic serum of types A, B, and E were introduced to neutralize the botulinum toxin in the test specimen. The serological reaction applied here involves the neutralization of the toxin by the specific antibodies present in the serum. This process prevents the toxin from causing harm to the mice. Summary of other choices: B: Precipitation - This involves the formation of insoluble complexes between antigen and antibody, not the specific neutralization seen in this case. C: Complement binding - This involves activation of the complement system leading to cell lysis, not the direct neutralization of toxin. D: Double immune diffusion - This technique is used to determine the presence of specific antibodies or antigens based on their diffusion patterns, not the neutralization of toxin as seen in this scenario.