Microscopic analysis of a smear stained by Ziehl-Neelsen method revealed bright red bacilli. What microorganism is being tested for?

Correct Answer: A

Rationale: The Ziehl-Neelsen stain is specific for acid-fast bacteria like Mycobacterium tuberculosis. The bright red bacilli seen in the smear indicate the presence of acid-fast organisms, which is characteristic of Mycobacterium tuberculosis. Staphylococcus aureus, Clostridium tetani, and Escherichia coli are not acid-fast bacteria and would not be stained red using the Ziehl-Neelsen method. Therefore, the correct answer is A: Mycobacterium tuberculosis.

Correct Answer: A

Rationale: The correct answer is A: Human papillomavirus. Genital warts are caused by certain strains of HPV, specifically types 6 and 11. HPV is a sexually transmitted infection that affects the skin and mucous membranes. HPV is directly linked to the development of genital warts through skin-to-skin contact during sexual activity. Herpes simplex virus 2 causes genital herpes, not genital warts. Human immunodeficiency virus (HIV) weakens the immune system but does not directly cause genital warts. Human cytomegalovirus (CMV) is not a common cause of genital warts. Therefore, HPV is the correct and most logical choice for the cause of genital warts.

Correct Answer: C

Rationale: The correct answer is C because coagulase helps bacteria like Staphylococcus aureus to form clots, allowing them to evade the host immune response. This mechanism helps the bacteria to hide from the immune system and establish infection. A. Adhesion to surfaces is usually facilitated by other factors like adhesins. B. Breaking down red blood cells is not a function of coagulase. D. Antibiotic resistance is typically mediated by other mechanisms like efflux pumps or enzymatic inactivation, not coagulase.

Correct Answer: A

Rationale: The correct answer is A: Mycobacterium tuberculosis. 1. Bright-red acid-resistant bacilli indicate the presence of mycolic acids in the cell wall, characteristic of Mycobacterium species. 2. Found in groups or singularly suggests the clumping nature of acid-fast bacilli. 3. Growth appearing after 10-15 days on nutrient media is consistent with the slow growth rate of Mycobacterium tuberculosis. 4. Yersinia pseudotuberculosis (B) and Klebsiella rhinoscleromatis (D) are not acid-fast bacilli and do not match the growth characteristics described. 5. Histoplasma dubrosii (C) is a fungus, not a bacterium, and does not exhibit acid-fast staining or the described growth pattern.

Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: C

Rationale: The correct answer is C because lymphocytic choriomeningitis virus belongs to the family Arenaviridae. This virus is a well-known member of the Arenaviridae family and is associated with various diseases. Option A is incorrect as Arenaviruses can be isolated from clinical materials using suitable methods. Option B is incorrect because Arenaviruses replicate in the cytoplasm, not the nucleus. Option D is incorrect as Arenaviruses can infect various hosts, not just humans. Therefore, the statement that lymphocytic choriomeningitis virus belongs to the family Arenaviridae is true.

Correct Answer: A

Rationale: The correct answer is A: Spores. In the Ozheshko staining method, spores appear blue with red terminal components. Spores are reproductive structures produced by some bacteria, fungi, and protozoa. Cilia are short, hair-like structures used for movement. Flagella are long, whip-like structures also used for movement. Capsules are outer protective layers surrounding some bacteria. In this case, the description of rod-like microorganisms with blue staining and red terminal components aligns with the characteristics of spores, making it the correct answer.

Correct Answer: C

Rationale: The correct answer is C: adhesion to surfaces and conjugation. Pili are hair-like structures on the surface of bacteria that help them adhere to surfaces and facilitate the transfer of genetic material during conjugation. This is essential for bacterial survival and reproduction. Choice A is incorrect because protection from desiccation is typically associated with other structures like capsules. Choice B is incorrect as motility in bacteria is usually facilitated by flagella. Choice D is incorrect as spore formation involves the formation of specialized structures, not pili.

Correct Answer: C

Rationale: The correct answer is C because cultural characteristics of bacteria refer to their growth patterns on different culture media, including liquid and solid agars. Bacteria exhibit unique growth patterns that can be observed and studied on these media. Choice A is incorrect as it refers to isolation techniques, not cultural characteristics. Choice B is incorrect as it describes metabolic activities, not cultural characteristics. Choice D is incorrect as it dismisses the essential aspect of bacterial growth on culture media.