Microbiological analysis of medicinal raw materials revealed capsular bacteria. What stain method was used to detect the capsules?

Correct Answer: A

Rationale: The correct answer is A: Gin's stain method. Gin's stain method is specifically used to detect capsules of bacteria. This method involves staining the bacteria with crystal violet and copper sulfate, which highlights the capsules as a clear halo around the stained bacterial cells. Ziehl-Neelsen's stain is used for acid-fast bacteria like Mycobacterium tuberculosis, Neisser's stain is for detecting Neisseria species, and Gram's stain is for differentiating bacteria based on their cell wall composition (Gram-positive vs. Gram-negative). Therefore, choices B, C, and D are incorrect for this scenario.

Correct Answer: B

Rationale: The correct answer is B: Clostridium tetani. Endospores are formed by certain bacteria as a survival mechanism in harsh conditions. Clostridium species, including C. tetani, have the ability to form endospores. Staphylococcus aureus (A), Neisseria gonorrhoeae (C), and Escherichia coli (D) do not produce endospores. Staphylococcus aureus is a gram-positive cocci bacterium, Neisseria gonorrhoeae is a gram-negative diplococci bacterium, and Escherichia coli is a gram-negative rod-shaped bacterium. Therefore, the correct choice is Clostridium tetani as it is known for its ability to form endospores.

Correct Answer: B

Rationale: The correct answer is B: Coxiella burnetii. Q-fever is caused by the bacterium Coxiella burnetii. It is not caused by viruses like Bunyaviridae or Filoviridae. Rickettsia conorii causes Mediterranean spotted fever, not Q-fever. Coxiella burnetii is the only bacterium among the choices known to be the etiological agent of Q-fever.

Correct Answer: A

Rationale: The correct answer is A: Yersinia pestis. This bacterium causes the plague, which presents with fever and swollen lymph nodes. The safety pin appearance on Gram stain is characteristic of Yersinia pestis. B: Francisella tularensis causes tularemia, which presents differently and does not show safety pin appearance on Gram stain. C: Brucella abortus causes brucellosis, which does not typically present with the same symptoms as described in the question. D: Clostridium botulinum causes botulism, which does not show safety pin appearance on Gram stain and does not typically present with fever and swollen lymph nodes.

Correct Answer: C

Rationale: Rationale: 1. Infectious process involves interactions between macro and microorganisms. 2. It is not limited to manifested diseases or typical symptoms. 3. The process can occur without leading to a full-blown infectious disease. 4. Choice C correctly captures the dynamic relationship between different organisms. Summary: - A: Incorrect - Infectious process is not solely about clinically manifested diseases. - B: Incorrect - Not all infectious diseases have typical symptoms. - D: Incorrect - Ambulatory form is a specific type, not encompassing all infectious processes.

Correct Answer: B

Rationale: Rationale: 1. The patient has symptoms of fever, enlarged lymph nodes, red and tender skin, which are indicative of systemic infection. 2. The inguinal lymph nodes in plague become enlarged, tender, and painful, forming buboes. 3. The presence of acute serohemorrhagic inflammation in the lymph node is characteristic of plague. 4. Plague is a zoonotic bacterial infection caused by Yersinia pestis, commonly transmitted through fleas from rodents. 5. Brucellosis, anthrax, and tularemia do not typically present with inguinal lymph node involvement and acute serohemorrhagic inflammation. Summary: - Brucellosis: Usually presents with flu-like symptoms, joint pain, and fever, not inguinal lymph node enlargement. - Anthrax: Presents with skin lesions, respiratory symptoms, or gastrointestinal symptoms, not inguinal lymph node involvement. - Tularemia: Presents with fever, skin ulcers,

Correct Answer: D

Rationale: The correct answer is D: chemoheterotrophs. Chemoheterotrophs use organic compounds as both their carbon source and energy source. They obtain energy by breaking down organic molecules through cellular respiration. Photoautotrophs (A) use light energy to produce organic compounds, chemoautotrophs (B) use inorganic compounds for energy, and photoheterotrophs (C) use light energy but require organic compounds as a carbon source. Therefore, chemoheterotrophs best describe bacteria that obtain both carbon and energy from organic compounds.

Correct Answer: A

Rationale: The correct answer is A: Artificial food media. Treponema pallidum is a fastidious bacterium that cannot be cultivated on artificial food media due to its complex nutritional requirements. It can be cultivated on cell lines and intratesticular in rabbits. Therefore, option A is incorrect. Option B and C are correct.

Correct Answer: A

Rationale: The correct answer is A: viral DNA polymerase. Herpesviruses require viral DNA polymerase for viral DNA replication. This enzyme is responsible for synthesizing new DNA strands using existing viral DNA as a template. Ribonucleotide reductase (B) is not directly involved in DNA replication but in the synthesis of deoxyribonucleotides. Neuraminidase (C) is an enzyme found in influenza viruses, not herpesviruses. Thymidine kinase (D) is involved in nucleotide metabolism but is not essential for viral DNA replication in herpesviruses.