Microbiological analysis of medicinal raw materials revealed capsular bacteria. What stain method was used to detect the capsules?

Correct Answer: A

Rationale: The correct answer is A: Gin's stain method. Gin's stain method is specifically used to detect capsules of bacteria. This method involves staining the bacteria with crystal violet and copper sulfate, which highlights the capsules as a clear halo around the stained bacterial cells. Ziehl-Neelsen's stain is used for acid-fast bacteria like Mycobacterium tuberculosis, Neisser's stain is for detecting Neisseria species, and Gram's stain is for differentiating bacteria based on their cell wall composition (Gram-positive vs. Gram-negative). Therefore, choices B, C, and D are incorrect for this scenario.

Correct Answer: A

Rationale: The correct answer is A: Streptomycin. Streptomycin is an aminoglycoside antibiotic used to treat tuberculosis that can cause hearing loss as a side effect. It is known to damage the cochlear and vestibular organs in the inner ear. The other choices, B: Isoniazid, C: Rifampicin, and D: Ethionamide, are not associated with causing hearing deterioration as a side effect. Isoniazid can rarely cause peripheral neuropathy, Rifampicin can lead to hepatotoxicity, and Ethionamide may cause gastrointestinal disturbances, but none of these drugs are known to specifically cause hearing loss.

Correct Answer: B

Rationale: The Widal test is used to diagnose typhoid fever caused by the bacteria Salmonella typhi. The test detects antibodies produced in response to the bacteria. Positive results indicate a current or past infection with typhoid fever. Choice A (Syphilis) is incorrect as it is diagnosed using different tests like RPR or TPPA. Choice C (AIDS) is diagnosed with HIV tests like ELISA or Western blot. Choice D (Lyme disease) is diagnosed using tests like ELISA or Western blot specific for Borrelia burgdorferi.

Correct Answer: A

Rationale: The correct answer is A: viral DNA polymerase. Herpesviruses require viral DNA polymerase for viral DNA replication. This enzyme is responsible for synthesizing new DNA strands using existing viral DNA as a template. Ribonucleotide reductase (B) is not directly involved in DNA replication but in the synthesis of deoxyribonucleotides. Neuraminidase (C) is an enzyme found in influenza viruses, not herpesviruses. Thymidine kinase (D) is involved in nucleotide metabolism but is not essential for viral DNA replication in herpesviruses.

Correct Answer: C

Rationale: The correct answer is C (Both A and B) because both Bacillus anthracis and Clostridium botulinum are capable of forming spores to survive in harsh environments. Bacillus anthracis causes anthrax and forms spores that can survive extreme conditions. Clostridium botulinum causes botulism and also forms spores for survival. Option A (Bacillus anthracis) and Option B (Clostridium botulinum) are incorrect on their own because they only represent one of the bacteria capable of forming spores. Option D (None of the above) is incorrect because it disregards the fact that both bacteria can form spores.

Correct Answer: D

Rationale: The correct answer is D: Hyaluronidase synthesis. Hyaluronidase is an enzyme produced by some pathogens to break down hyaluronic acid in the extracellular matrix, aiding their spread. This action does not directly counteract the immune system's mechanisms. Antigenic heterogeneity and mimicry can evade immune recognition. IgA proteases degrade IgA antibodies, impairing mucosal immunity. Therefore, D is the only choice not directly involved in counteracting the immune system.

Correct Answer: A

Rationale: The correct answer is A: T-lymphocytes. In skin mycosis, a disorder of cellular immunity indicates a deficiency in T-lymphocytes, which play a crucial role in cell-mediated immunity. T-lymphocytes are responsible for recognizing and destroying infected cells. Reduction in T-lymphocytes impairs the body's ability to fight fungal infections. Incorrect choices: B: Immunoglobulin G - Immunoglobulin G is involved in humoral immunity, not cellular immunity. C: Immunoglobulin E - Immunoglobulin E is typically associated with allergic responses, not cellular immunity. D: B-lymphocytes - B-lymphocytes are responsible for producing antibodies in humoral immunity, not directly involved in cell-mediated immunity.

Correct Answer: B

Rationale: Facultative anaerobes can switch between aerobic and anaerobic metabolism based on oxygen availability. This flexibility enables them to grow in both oxygen-rich and oxygen-poor environments, making choice B correct. Choice A is incorrect because facultative anaerobes do not require oxygen for survival. Choice C is incorrect as obligate anaerobes cannot grow in the presence of oxygen. Choice D is incorrect because obligate anaerobes cannot tolerate any amount of oxygen.

Correct Answer: A

Rationale: The correct answer is A: Artificial food media. Treponema pallidum is a fastidious bacterium that cannot be cultivated on artificial food media due to its complex nutritional requirements. It can be cultivated on cell lines and intratesticular in rabbits. Therefore, option A is incorrect. Option B and C are correct.