Identify the correct statement(s). In eukaryotic microorganisms:

Correct Answer: D

Rationale: The correct answer is D because extrachromosomal DNA, such as plasmids, can be present in eukaryotic microorganisms. This is true for certain organisms like yeast and some fungi. These DNA molecules are separate from the main chromosomal DNA and can carry additional genetic information. A: mRNA in eukaryotic microorganisms is not short-lived; it can have varying lifespans depending on the specific mRNA molecule and cellular conditions. B: Eukaryotic microorganisms typically have multiple linear chromosomes, not a single circular chromosome. C: While 5s, 16s, and 23s ribosomal RNAs are present in prokaryotic organisms, eukaryotic microorganisms have different types of ribosomal RNA subunits.

Correct Answer: B

Rationale: The correct answer is B: Class 2. Class 2 restriction endonucleases are commonly used in recombinant DNA technology due to their specificity for recognizing short DNA sequences and cleaving within or near these sequences. They are commercially available, easy to use, and have well-defined recognition sites, making them ideal for molecular cloning applications. Choice A (Class 1) is incorrect because Class 1 enzymes require both ATP and S-adenosyl methionine for activity, making them less practical for routine laboratory use. Choice C (Class 3) is incorrect because Class 3 enzymes cleave DNA at two sites, potentially leading to unwanted DNA fragmentation in recombinant DNA technology. Choice D (Class 5) is incorrect because Class 5 restriction enzymes do not exist in the classification system of restriction endonucleases.

Correct Answer: D

Rationale: The correct answer is D because BLASTN searches nucleotide databases, not protein databases. BLASTN uses a nucleotide sequence as the query to find similar sequences in nucleotide databases. This is different from BLASTX, which searches protein databases using translated nucleotide sequences (answer A is correct). TBLASTX and TBLASTN translate the query sequence into six reading frames to search for similarities in both nucleotide and protein databases (answer C is correct). BLASTN is specifically designed for nucleotide sequence searches, making statement D incorrect.

Correct Answer: C

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.

Correct Answer: B

Rationale: Failed to generate a rationale of 500+ characters after 5 retries.