How the prepared immunofluorescence slide should be examined?

Correct Answer: A

Rationale: The correct answer is A because immunofluorescence slides are examined under a fluorescence microscope with a x100 oil immersion objective. This is because immunofluorescence relies on the detection of fluorescently labeled antibodies, which emit light when excited by a specific wavelength. Using a fluorescence microscope allows for visualization of these labeled antibodies at high magnification and resolution, enabling precise examination of cellular structures and protein localization. Choice B is incorrect as light microscopes are not suitable for visualizing fluorescence. Choice C is incorrect as electron microscopes use electrons, not light, for imaging, which is not compatible with immunofluorescence. Choice D is incorrect as there is a specific method for examining immunofluorescence slides.

Correct Answer: B

Rationale: The correct answer is B: Herpangina. Coxsackie Viruses are known to cause Herpangina, which is characterized by sore throat, fever, and painful blisters in the mouth and throat. This is because Coxsackie Viruses typically infect the throat and mouth mucosa. Hemorrhagic conjunctivitis (A) is commonly caused by Adenoviruses, not Coxsackie Viruses. Mumps (C) is caused by the Mumps virus, a completely different virus. Poliomyelitis (D) is caused by the Poliovirus, not Coxsackie Viruses. Therefore, the correct answer is B as it aligns with the known pathogenicity of Coxsackie Viruses.

Correct Answer: C

Rationale: The correct answer is C: Hepatitis A virus. This is because Hepatitis A virus is a non-enveloped virus, while choices A, B, and D are all enveloped viruses. Enveloped viruses have a lipid bilayer membrane surrounding their protein capsid, aiding in their ability to infect host cells. Hepatitis C, B, and D viruses all possess this lipid envelope, making them enveloped viruses. Hepatitis A virus, on the other hand, lacks this lipid envelope and is classified as a non-enveloped virus. Therefore, the correct answer is C.

Correct Answer: A

Rationale: The correct answer is A because spirochaetes are not bacilli; they are spiral-shaped bacteria. Bacilli are rod-shaped bacteria, making statement A false. Statement B is true as diplococci are spherical bacteria occurring in pairs. Statement C is true as rod-shaped bacteria have one diameter longer than the other. Statement D is true as coccobacilli are short, oval-shaped bacteria resembling cocci and rods. In summary, spirochaetes are spiral-shaped, not bacilli, making statement A false.

Correct Answer: C

Rationale: The correct answer is C: Method of Moller. This method uses malachite green to stain bacterial spores due to their resistance to decolorization. The other choices, A, B, and D, are incorrect because Romanovski-Gymsa is used for staining blood smears, Ziehl-Nielsen is used for acid-fast bacteria, and Neisser is used for staining Neisseria species, not bacterial spores.