Correct Answer: D

Rationale: The correct answer is D because viral multiplication in cell culture can be detected by observing a specific cytopathic effect (A) caused by viral infection in cells and by using an immunofluorescence detection method (B) to visualize viral antigens within the cells. Choice C, the characteristic type of viral colonies, is incorrect as viral multiplication is not typically detected by observing viral colonies in cell culture. Option D is the correct choice as both A and B methods are commonly used to detect viral multiplication in cell culture.

Correct Answer: A

Rationale: The correct answer is A: They are enveloped viruses. Rhabdoviridae family consists of enveloped viruses, which means they have a lipid envelope surrounding their protein coat. This envelope helps the virus in infecting host cells and evading the immune system. The other choices are incorrect because B: They cause plague is not true as Rhabdoviridae family typically causes diseases like rabies in mammals. C: They have spherical shape is incorrect as rhabdoviruses have a bullet-shaped structure. D: They infect only humans is also incorrect as Rhabdoviridae viruses can infect a wide range of hosts beyond just humans.

Correct Answer: A

Rationale: The correct answer is A: Trichomonas vaginalis. Trichomonas vaginalis is a sexually transmitted protozoan parasite that causes trichomoniasis, characterized by foamy purulent vaginal discharges. Microscopy of vaginal discharge showing flagellated protozoa with an undulating membrane and pear-shaped structure is indicative of Trichomonas vaginalis. Trypanosoma gambiense causes African sleeping sickness and is transmitted by tsetse flies. Leishmania donovani causes visceral leishmaniasis transmitted by sandflies. Lamblia intestinalis causes giardiasis and is characterized by diarrhea and flatulence.

Correct Answer: A

Rationale: The correct answer is A, Exotoxin. Botulism is caused by the exotoxin produced by Clostridium botulinum bacteria. The symptoms described - diplopia, acute headache, deglutition disorder, hard breathing, muscle weakness - are all consistent with botulism due to the neurotoxic effects of the exotoxin. The exotoxin interferes with neurotransmission at the neuromuscular junction, leading to muscle paralysis and the symptoms mentioned. The other choices (B, C, D, E) are incorrect as they do not correspond to the pathogenic mechanism of botulism and do not explain the specific clinical manifestations observed in this case.

Correct Answer: A

Rationale: The correct answer is A because adaptive immunity is not mainly observed in the skin and mucous membranes. Adaptive immunity is a systemic response that involves specific immune cells and antibodies targeting specific pathogens. It develops immune memory (B), meaning it can recognize and respond faster upon re-exposure to the same pathogen. Adaptive immunity also has specificity (C), as it can target specific antigens. Lastly, adaptive immunity is not evolutionarily newer in comparison to innate immunity (D), as both systems have co-evolved to provide comprehensive protection against pathogens.