How is detected viral multiplication in cell culture

Correct Answer: D

Rationale: The correct answer is D (A and B both). In viral multiplication, the presence of a specific cytopathic effect (CPE) in cell culture indicates viral replication. This is detected visually through changes in cell morphology. Additionally, immunofluorescence detection methods can be used to detect viral antigens within infected cells, confirming viral multiplication. Options C is incorrect as viral colonies are not typically used to detect viral multiplication in cell culture. Option B alone is not sufficient as it only focuses on immunofluorescence detection, while option A alone may not always be specific. Therefore, the combination of A and B provides a more comprehensive and accurate detection method for viral multiplication in cell culture.

Correct Answer: A

Rationale: The correct answer is A: chronic. A persistent infection that is not lytic but productive is characterized by ongoing viral replication without cell lysis. This type of infection is referred to as chronic, as it persists over an extended period. Latent infections involve periods of dormancy with intermittent reactivation. Slow infections typically have a prolonged incubation period before causing symptoms. Transforming infections lead to permanent alterations in host cell behavior. The key distinction for this question is the continuous viral replication without cell destruction, which aligns with the definition of a chronic infection.

Correct Answer: A

Rationale: The correct answer is A because the secondary immune response is quicker due to memory B and T cells being already present from the primary response. These memory cells can quickly recognize and respond to the antigen upon re-exposure, leading to a faster and more efficient immune response. Choices B and C are incorrect as the secondary response produces more antibodies and requires fewer antigen stimulations. Choice D is also incorrect as the secondary response is faster than the primary response.

Correct Answer: A

Rationale: The correct answer is A: Sabouraud's peptone agar. This agar is specifically designed for the isolation of yeast and molds due to its low pH and high sugar content, creating an environment conducive for their growth. Yeast-like fungi thrive in this selective medium, while inhibiting the growth of bacteria and other contaminants. Summary: - Sabouraud's peptone agar is ideal for cultivating yeast-like fungi. - Endo agar is used for isolation of gram-negative bacteria, not fungi. - Milk-salt agar is used for lactobacilli and streptococci, not fungi. - Meat infusion agar is a general-purpose medium for cultivation of a wide range of microorganisms, not specifically for yeast-like fungi.

Correct Answer: C

Rationale: Rationale: Metronidazole is the drug of choice for systemic amebiasis due to its high efficacy against Entamoeba histolytica. It has good tissue penetration and can treat intestinal, liver, and lung involvement. Quiniofone (A) has limited use and is not recommended for amebiasis. Enteroseptol (B) is not effective against systemic amebiasis. Tetracycline (D) is not the first-line treatment for amebiasis and has limited efficacy against Entamoeba histolytica.

Correct Answer: A

Rationale: Rationale: The correct answer is A: Trichomonas vaginalis. 1. Symptoms of urogenital inflammation match Trichomonas vaginalis infection. 2. Description matches Trichomonas vaginalis characteristics: pear-shaped, pointed spike, big nucleus, undulating membrane. 3. Trichomonas hominis and buccalis do not typically infect urogenital tracts. 4. Trypanosoma gambiense causes African trypanosomiasis, not urogenital infections.

Correct Answer: B

Rationale: The correct answer is B: presence of metachromatic granules. This is because Corynebacterium diphtheriae is known for containing metachromatic granules, which are characteristic of this species. These granules can be observed under a microscope and aid in the identification of the bacteria. Explanation of other choices: A: formation of spores - Corynebacterium diphtheriae does not form spores. Spore formation is more commonly seen in bacteria such as Bacillus and Clostridium species. C: production of coagulase - Coagulase production is a characteristic of Staphylococcus aureus, not Corynebacterium diphtheriae. D: beta-hemolysis on blood agar - Corynebacterium diphtheriae does not exhibit beta-hemolysis on blood agar. This type of hemolysis is more typical of Streptococcus pyogenes.

Correct Answer: A

Rationale: The correct answer is A: cryophiles. Psychrophiles are microorganisms that thrive in cold temperatures. The prefix "cryo-" means cold, which accurately describes the environment in which these organisms grow. Choice B, thermophiles, are organisms that thrive in high temperatures. Choice C, mesophiles, grow best at moderate temperatures. Choice D, psychrotrophs, are organisms that can grow at refrigeration temperatures but have an optimal growth temperature range different from psychrophiles.

Correct Answer: C

Rationale: The correct answer is C: cell wall composition. Gram staining differentiates bacteria based on the structure of their cell walls. It classifies bacteria into Gram-positive (thick peptidoglycan layer) and Gram-negative (thin peptidoglycan layer). This differentiation is crucial for identifying bacterial species and guiding treatment choices. Size and shape (choice A) are not specific to Gram staining. Presence of flagella (choice B) is related to bacterial motility, not Gram staining. Genetic material (choice D) is not directly assessed by Gram staining. Overall, the unique composition of the cell wall is the key factor in distinguishing bacteria through Gram staining.