Gram-positive cocci arranged in chains were found in a wound smear. The bacteria were catalase-negative and beta-hemolytic on blood agar. What is the causative agent?

Correct Answer: A

Rationale: The correct answer is A: Streptococcus pyogenes. This bacterium fits all the given characteristics: Gram-positive cocci arranged in chains, catalase-negative, and beta-hemolytic on blood agar. Streptococcus pyogenes is known for causing skin and soft tissue infections. Staphylococcus aureus (B) is catalase-positive and typically forms clusters, not chains. Enterococcus faecalis (C) is catalase-negative but not beta-hemolytic. Micrococcus luteus (D) is catalase-positive and not typically associated with wound infections.

Correct Answer: A

Rationale: The correct answer is A: Pseudomonas aeruginosa. This is because Pseudomonas is a Gram-negative rod, oxidase-positive, and non-lactose fermenter. These characteristics are typical of Pseudomonas species. B: Escherichia coli is a Gram-negative rod but is lactose fermenter and usually oxidase-negative. C: Klebsiella pneumoniae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative. D: Shigella dysenteriae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative and is more associated with causing shigellosis rather than deep tissue infections.

Correct Answer: A

Rationale: Step-by-step rationale: 1. IgG is the most abundant immunoglobin in a normal healthy adult due to its long half-life and diverse functions. 2. It plays a crucial role in immune response by neutralizing pathogens and enhancing phagocytosis. 3. IgA is mainly found in mucosal areas, IgE is involved in allergic reactions, and IgD's function is not fully understood. 4. Therefore, IgG is the correct answer as it is the predominant immunoglobin in circulation, providing broad immune protection.

Correct Answer: C

Rationale: The correct answer is C: Salmonella. This bacterium should be handled in a Biosafety Level 2 facility due to its potential to cause disease in humans. Salmonella is a pathogenic bacterium that can cause foodborne illnesses and infections. Handling it in a Biosafety Level 2 facility ensures proper containment and protection of personnel. A: Micrococcus luteus and B: Bacillus subtilis are common environmental bacteria that do not pose significant risks to human health, so they can be safely handled in lower biosafety levels. D: Staphylococcus epidermidis is a common skin commensal bacterium that is generally not considered highly pathogenic, so it does not require Biosafety Level 2 containment.

Correct Answer: A

Rationale: The correct answer is A: Neutralization. In this scenario, the antibotulinic serum of types A, B, and E were introduced to neutralize the botulinum toxin in the test specimen. The serological reaction applied here involves the neutralization of the toxin by the specific antibodies present in the serum. This process prevents the toxin from causing harm to the mice. Summary of other choices: B: Precipitation - This involves the formation of insoluble complexes between antigen and antibody, not the specific neutralization seen in this case. C: Complement binding - This involves activation of the complement system leading to cell lysis, not the direct neutralization of toxin. D: Double immune diffusion - This technique is used to determine the presence of specific antibodies or antigens based on their diffusion patterns, not the neutralization of toxin as seen in this scenario.

Correct Answer: A

Rationale: The correct answer is A: Staphylococcus aureus and hemolytic streptococcus. These microorganisms are commonly found in high concentrations during sanitary and bacteriological examination of air in a drugstore due to their prevalence in the environment. Staphylococcus aureus and hemolytic streptococcus are known to be significant pathogens that can cause various infections in humans. Rationale: 1. Staphylococcus aureus and hemolytic streptococcus are common pathogens found in the environment and can easily contaminate air in enclosed spaces like a drugstore. 2. Both microorganisms are considered to be of sanitary significance as they can cause infections and are indicators of poor hygiene and sanitation. 3. Diphtheritic and tuberculous bacilli (Choice B) are not typically associated with air contamination in a drugstore setting. 4. Colibacilli and blue pus bacilli (Choice C) are more commonly found in fecal

Correct Answer: C

Rationale: Step 1: "Fried egg" colonies typically refer to colonies with a raised center and flattened periphery, resembling the appearance of a fried egg. Step 2: Actinomycetes are filamentous bacteria known to form "fried egg" colonies in nutrient medium. Step 3: Actinomycetes are commonly found in soil and are known to be phytopathogenic microorganisms. Step 4: Actinomycetes produce secondary metabolites with medicinal properties, making them a likely agent from a medicinal herb. Overall, the correct answer is C (Actinomycetes) as they fit the description provided in the question and are commonly associated with the given scenario. The other choices are incorrect as they do not exhibit the specific characteristics mentioned in the question or are not typically associated with forming "fried egg" colonies in nutrient medium.

Correct Answer: A

Rationale: The correct answer is A: Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative rod that is a lactose non-fermenter commonly associated with deep tissue infections. It is an opportunistic pathogen known for its ability to cause infections in immunocompromised individuals. P. aeruginosa is often resistant to multiple antibiotics and can form biofilms, making it difficult to treat. Summary of why other choices are incorrect: B: Escherichia coli is a Gram-negative rod that ferments lactose and is commonly found in the gastrointestinal tract. It is not typically associated with deep tissue infections. C: Salmonella typhi is a Gram-negative rod that causes typhoid fever, primarily affecting the gastrointestinal system, not deep tissue infections. D: Shigella dysenteriae is a Gram-negative rod that causes bacillary dysentery, which primarily affects the gastrointestinal tract and does not typically cause deep tissue infections.

Correct Answer: A

Rationale: The correct answer is A: Capnophilic. Brucella requires a CO2 enriched atmosphere for cultivation, making it capnophilic. This is because Brucella is a microaerophilic bacterium that thrives in low oxygen conditions and elevated CO2 levels. Choice B (Facultative anaerobic) and D (Obligate aerobic) are incorrect as Brucella does not grow well under aerobic conditions. Choice C (Obligate anaerobic) is also incorrect as Brucella can grow in the presence of oxygen, although it prefers low oxygen environments.