For which family the following morphological features are typical: filamentous spiral nucleocapsid, linear unsegmented single strand (-) RNA and lipid bilayer membrane of cellular origin:

Correct Answer: B

Rationale: Step-by-step rationale: 1. Filamentous spiral nucleocapsid is characteristic of Filoviridae, such as Ebola virus. 2. Linear unsegmented single strand (-) RNA is a key feature of Filoviridae genomes. 3. The lipid bilayer membrane of cellular origin is found in Filoviridae viruses. 4. Therefore, the correct answer is B (Filoviridae) due to the alignment of all three morphological features. Summary: - A (Picornaviridae): Has an icosahedral capsid, not filamentous spiral. - C (Orthomyxoviridae): Has a segmented genome, not linear unsegmented. - D (Togaviridae): Has a spherical capsid, not filamentous spiral.

Correct Answer: B

Rationale: The correct answer is B: Corynebacterium diphtheriae. This bacterium produces a toxin that causes diphtheria. It forms a grayish membrane in the throat, leading to difficulty breathing. Bacillus anthracis (Choice A) causes anthrax, Streptococcus pneumoniae (Choice C) causes pneumonia, and Escherichia coli (Choice D) causes various infections but not diphtheria.

Correct Answer: A

Rationale: Step 1: Gram-negative rod with safety pin appearance indicates bipolar staining. Step 2: Yersinia pestis is a Gram-negative rod causing bubonic plague. Step 3: Y. pestis has bipolar staining due to its unique F1 capsule. Step 4: The swollen lymph node and bacteremia are consistent with bubonic plague. Step 5: Therefore, the correct answer is A: Yersinia pestis. Summary: - B: Clostridium tetani causes tetanus, not characterized by a safety pin appearance. - C: Brucella melitensis causes brucellosis, not characterized by a safety pin appearance. - D: Pasteurella multocida causes animal bites infections, not characterized by a safety pin appearance.

Correct Answer: D

Rationale: The correct answer is D (A and B both). In viral multiplication, the presence of a specific cytopathic effect (CPE) in cell culture indicates viral replication. This is detected visually through changes in cell morphology. Additionally, immunofluorescence detection methods can be used to detect viral antigens within infected cells, confirming viral multiplication. Options C is incorrect as viral colonies are not typically used to detect viral multiplication in cell culture. Option B alone is not sufficient as it only focuses on immunofluorescence detection, while option A alone may not always be specific. Therefore, the combination of A and B provides a more comprehensive and accurate detection method for viral multiplication in cell culture.

Correct Answer: C

Rationale: The correct answer is C: Identification. Bacteria are studied by identification to determine their specific characteristics and differentiate between different species. Taxonomy (A) is the science of classification, not specific to bacteria. Classification (B) is the process of grouping organisms based on shared characteristics, not specific to bacteria. Nomenclature (D) is the naming system for organisms, not the primary method for studying bacteria. Therefore, identification (C) is the correct choice for studying bacteria.

Correct Answer: D

Rationale: The correct answer is D. Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa all produce endotoxins that can lead to septic shock. Endotoxins are part of the outer membrane of Gram-negative bacteria and can trigger a systemic inflammatory response if released into the bloodstream. E. coli and Salmonella are common causes of bacterial infections that can lead to septic shock, while P. aeruginosa is a known opportunistic pathogen that can produce endotoxins. Therefore, all three bacteria can cause septic shock by releasing endotoxins. Choices A, B, and C are incorrect because each of these bacteria individually can produce endotoxins that contribute to the development of septic shock.

Correct Answer: B

Rationale: The correct answer is B because filoviruses, such as Ebola virus, require investigation in specifically equipped laboratories with high biological protection due to their high pathogenicity and risk of transmission. This is essential for ensuring the safety of researchers and preventing outbreaks. A: This is incorrect as filoviruses are not the most common cause of gastroenteritis; they primarily cause severe hemorrhagic fevers. C: Filoviruses are not widespread; they are typically confined to specific regions during outbreaks. D: Mass immunization is not used for filoviruses as there is no established vaccine for general prophylaxis.

Correct Answer: B

Rationale: Step-by-step rationale: 1. Marburg virus is named after the German city Marburg where it was first identified in 1967. 2. Chikungunya virus is named after a village in Tanzania. 3. Measles virus got its name from the Middle English word "maselen" meaning "many little spots". 4. Epstein-Barr virus is named after Michael Epstein and Yvonne Barr, who discovered it. Summary: B is correct as it is directly named after the city of its discovery. A, C, and D are incorrect as they are named after other locations, characteristics, or discoverers.

Correct Answer: B

Rationale: The correct answer is B: cell wall. Lysozyme is an enzyme that targets the cell wall of bacteria by breaking down the peptidoglycan layer, leading to bacterial cell lysis. This action disrupts the structural integrity of the bacteria, ultimately causing their death. Choices A, C, and D are incorrect because lysozyme does not target the cytoplasm, nucleus, or capsule of bacteria. The primary function of lysozyme is to act on the cell wall, making option B the correct answer in this context.