Correct Answer: B

Rationale: The correct answer is B: autoclaving. Autoclaving is a process that uses high pressure and steam to achieve temperatures above the boiling point of water, typically around 121-134°C. This high heat and pressure are effective in destroying spore forms of microorganisms, which are highly resistant structures that can withstand boiling temperatures. Autoclaving ensures the complete destruction of spores, making it an effective method for sterilization. Rationale: A: Pasteurization uses lower heat compared to autoclaving and is not sufficient to destroy spore forms. C: Freezing does not effectively destroy spore forms, as most microorganisms can survive freezing temperatures. D: Boiling, while effective for many microorganisms, may not be sufficient to destroy spore forms due to their high resistance, unlike autoclaving.

Correct Answer: A

Rationale: Rationale: 1. Dysenteric amoeba (Choice A) is the correct answer because it can exist asymptomatically in carriers and be transmitted through cysts. 2. Malarial plasmodium (Choice B) causes malaria, not carried asymptomatically in cysts. 3. Intestinal trichomonad (Choice C) causes trichomoniasis, not carried asymptomatically in cysts. 4. Dermatotropic leishmania (Choice D) causes skin lesions, not carried asymptomatically in cysts.

Correct Answer: D

Rationale: The correct answer is D: HIV. HIV is transmitted through direct contact with certain body fluids such as blood, semen, vaginal fluids, and breast milk. This direct contact route of transmission makes it different from the other choices. Salmonellosis is typically transmitted through contaminated food or water (not direct contact). Tularemia is usually transmitted through insect bites or contact with infected animals. Rubella is primarily transmitted through respiratory droplets. Therefore, HIV is the only option with a contact route of transmission.

Correct Answer: D

Rationale: The correct answer is D because all of these symptoms are early signs of rabies. A short period of depression, nausea, and restlessness are common initial symptoms of rabies infection. These symptoms may be followed by more severe manifestations, such as aggression, confusion, and difficulty swallowing. Therefore, option D is the correct choice as it encompasses all the early symptoms of rabies. Options A, B, and C are incorrect as they focus on individual symptoms, whereas rabies typically presents with a combination of these early signs.

Correct Answer: A

Rationale: The correct answer is A: Bacteriological. This method involves isolating and culturing the causative bacteria from the wound material to determine the species. This allows for further testing to confirm gaseous gangrene and identify the specific pathogen. Choice B: Allergic is incorrect as it pertains to immune responses, not identifying bacteria. Choice C: Bacterioscopic is incorrect as it refers to microscopic examination of bacteria, not species identification. Choice D: Serological is incorrect as it involves testing for antibodies in the blood, not identifying the causative agent directly.