For destruction of spore forms of microorganisms is used:

Correct Answer: B

Rationale: The correct answer is B: autoclaving. Autoclaving is a process that uses high pressure and steam to achieve temperatures above the boiling point of water, typically around 121-134°C. This high heat and pressure are effective in destroying spore forms of microorganisms, which are highly resistant structures that can withstand boiling temperatures. Autoclaving ensures the complete destruction of spores, making it an effective method for sterilization. Rationale: A: Pasteurization uses lower heat compared to autoclaving and is not sufficient to destroy spore forms. C: Freezing does not effectively destroy spore forms, as most microorganisms can survive freezing temperatures. D: Boiling, while effective for many microorganisms, may not be sufficient to destroy spore forms due to their high resistance, unlike autoclaving.

Correct Answer: D

Rationale: The correct answer is D because all of the symptoms mentioned (short period of depression, nausea, restlessness) can be early signs of rabies. Rabies typically presents with a variety of non-specific symptoms in the early stages, including behavioral changes such as depression and restlessness, as well as gastrointestinal symptoms like nausea. Therefore, it is important to consider the combination of these symptoms collectively rather than individually when suspecting rabies. Choices A, B, and C alone may not definitively indicate rabies, but when seen together, they should raise suspicion and prompt further evaluation and testing for rabies.

Correct Answer: C

Rationale: The correct answer is C: Polar placement of volutin granules. This feature is indicative of Corynebacterium diphtheria. Volutin granules are metachromatic granules found in the polar region of the bacterial cell, which is characteristic of this species. This feature helps differentiate it from other bacteria. Explanation: A: Fence-like position of the agent's cells is not a specific morphologic feature of Corynebacterium diphtheria. B: Spores that exceed cells in diameter is not a characteristic feature of Corynebacterium diphtheria. D: Presence of the capsule is not a distinguishing morphologic feature of Corynebacterium diphtheria. In summary, the polar placement of volutin granules is the most indicative feature for determining the type of agent as Corynebacterium diphtheria, making it the correct choice.

Correct Answer: A

Rationale: The correct answer is A: Capsule. The capsule is a mucous structure tightly bound to the bacterial cell wall. It helps bacteria evade the host immune system and provides protection. The other choices are incorrect because: B) Spores are resistant structures formed by some bacteria for survival, C) Flagella are long, whip-like structures for movement, and D) Filaments are long, thin structures found in some bacteria.

Correct Answer: B

Rationale: The correct answer is B because the presence of calcium dipicolinate in bacterial spores helps protect their DNA from damage under harsh conditions. Calcium dipicolinate acts as a stabilizer, preserving the spore structure. Choice A is incorrect as bacterial spores have a tough protein coat, not a lipid-rich membrane. Choice C is incorrect as bacterial spores have very low metabolic activity. Choice D is incorrect as bacterial spores have low water content to prevent damage during harsh conditions.