For destruction of spore forms of microorganisms is used:

Correct Answer: B

Rationale: The correct answer is B: autoclaving. Autoclaving is a process that uses high pressure and steam to achieve temperatures above the boiling point of water, typically around 121-134°C. This high heat and pressure are effective in destroying spore forms of microorganisms, which are highly resistant structures that can withstand boiling temperatures. Autoclaving ensures the complete destruction of spores, making it an effective method for sterilization. Rationale: A: Pasteurization uses lower heat compared to autoclaving and is not sufficient to destroy spore forms. C: Freezing does not effectively destroy spore forms, as most microorganisms can survive freezing temperatures. D: Boiling, while effective for many microorganisms, may not be sufficient to destroy spore forms due to their high resistance, unlike autoclaving.

Correct Answer: B

Rationale: The correct answer is B because the presence of calcium dipicolinate in bacterial spores helps protect their DNA from damage under harsh conditions. Calcium dipicolinate acts as a stabilizer, preserving the spore structure. Choice A is incorrect as bacterial spores have a tough protein coat, not a lipid-rich membrane. Choice C is incorrect as bacterial spores have very low metabolic activity. Choice D is incorrect as bacterial spores have low water content to prevent damage during harsh conditions.

Correct Answer: A

Rationale: The correct answer is A: Bacterial ribosomes synthesize proteins. Ribosomes are cellular organelles responsible for protein synthesis by translating mRNA into amino acids. They are essential for the production of proteins needed for cell function. Choices B, C, and D are incorrect because ribosomes do not synthesize DNA, degrade toxic substances, or assist in cell division. DNA synthesis is carried out by DNA polymerase, degradation of toxic substances is typically done by enzymes, and cell division is primarily regulated by the cell cycle machinery.

Correct Answer: B

Rationale: The correct answer is B: Thermophiles. Thermophiles are bacteria that can survive in extreme temperatures, typically between 45°C to 80°C. This ability is due to their unique enzymes and cell membrane structures that are adapted to high temperatures. Escherichia coli (A) is a common bacterium found in the human gut and does not thrive in extreme temperatures. Clostridium tetani (C) is a bacterium that causes tetanus and prefers moderate temperatures. Salmonella enterica (D) is a pathogenic bacterium that does not thrive in extreme temperatures. Therefore, Thermophiles are the only bacteria on the list known for surviving in extreme temperatures.

Correct Answer: A

Rationale: The correct answer is A. Method of Ziel-Neelsen. This method uses a special stain (carbol fuchsin) to detect acid-fast organisms like Mycobacterium tuberculosis, the causative agent of tuberculosis. The staining procedure involves heating the slide to help penetrate the mycolic acid layer of the bacteria, making them resistant to decolorization by acid-alcohol. The red stained acid-fast bacilli stand out against the blue counterstain. This method is specific for identifying tubercle bacilli in sputum samples. Choice B: Method of Romanowsky-Giemsa is used for staining blood smears and is not suitable for detecting acid-fast bacilli in sputum. Choice C: Method of Gram is used for staining bacteria based on their cell wall composition and is not effective for identifying acid-fast bacilli like Mycobacterium tuberculosis. Choice D: Method of Neisser is not a specific staining method for acid-fast bacilli and is not suitable

Correct Answer: A

Rationale: Rationale: Bacillus cereus is associated with food intoxication due to its ability to produce toxins that cause gastrointestinal symptoms. B. subtilis is not known to cause food intoxication. B. anthracis causes anthrax, not food intoxication. Option D is incorrect as not all Bacillus species cause food intoxication.

Correct Answer: C

Rationale: The correct answer is C: crateriform. In a gelatin stab, a saucer-shaped liquefaction indicates a depression or crater-like shape. This term accurately describes the appearance of the liquefaction. A: napiform means turnip-shaped, which is not relevant to the shape in a gelatin stab. B: saccate means pouch-like, which does not match the saucer-shaped description. D: stratiform refers to something in layers, which is not applicable to the shape of the liquefaction. Therefore, C is the most appropriate term to describe the saucer-shaped liquefaction in a gelatin stab.

Correct Answer: C

Rationale: To calculate the total magnification, we multiply the magnification of the ocular lens by the magnification of the objective lens. In this case, 10 (ocular) x 40 (objective) = 400. This is why choice C (400) is correct. Choices A, B, and D are incorrect because they do not represent the correct calculation of total magnification based on the given magnification powers of the ocular and objective lenses.

Correct Answer: D

Rationale: The correct answer is D because all of the symptoms mentioned (short period of depression, nausea, restlessness) can be early signs of rabies. Rabies typically presents with a variety of non-specific symptoms in the early stages, including behavioral changes such as depression and restlessness, as well as gastrointestinal symptoms like nausea. Therefore, it is important to consider the combination of these symptoms collectively rather than individually when suspecting rabies. Choices A, B, and C alone may not definitively indicate rabies, but when seen together, they should raise suspicion and prompt further evaluation and testing for rabies.