Ebola viruses need to be handled in which of the following biosafety levels?

Correct Answer: D

Rationale: The correct answer is D: BSL4. Ebola viruses are highly infectious and pose a high risk to laboratory workers. BSL4 facilities are designed to handle agents that pose a severe threat to human health and have no available vaccines or treatments. These facilities have strict guidelines for handling infectious agents, including full-body suits, negative air pressure, and decontamination processes to prevent any possible exposure. BSL1, BSL2, and BSL3 are not adequate for handling Ebola viruses due to their lower levels of containment and safety measures.

Correct Answer: A

Rationale: The correct answer is A: Nystatin. Nystatin is an antifungal medication, not an antibiotic used to treat bacterial infections like generalized meningococcal infection. Penicillin G, macrolides, and chloramphenicol are antibiotics commonly used to treat bacterial infections, including meningococcal infections. Penicillin G is the drug of choice for meningococcal infections, while macrolides and chloramphenicol are alternative options. Nystatin is not effective against bacteria, so it is not a suitable treatment for meningococcal infections.

Correct Answer: C

Rationale: Rationale: 1. Both Clostridium botulinum and Bacillus anthracis are known to form spores. 2. Spore formation is a mechanism for survival in harsh conditions. 3. Both bacteria are capable of causing severe diseases in humans. Summary: - Choice A and B are incorrect because each bacteria listed is capable of spore formation. - Choice D is incorrect as both bacteria mentioned can form spores to survive extreme conditions.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Anopheles mosquitoes are the primary vectors for transmitting malaria. 2. They prefer stagnant water for breeding, common in tropical regions. 3. Culex mosquitoes primarily spread diseases like West Nile virus. 4. Aedes mosquitoes transmit diseases like Zika and dengue fever. 5. Mansonia mosquitoes are known for transmitting filarial diseases. Therefore, the correct answer is A (Anopheles), as they are specifically associated with spreading malaria.

Correct Answer: B

Rationale: The correct answer is B: Motility. The bacterial flagellum is a whip-like appendage that helps bacteria move by rotating like a propeller. This motility allows bacteria to navigate their environment, find nutrients, and avoid harmful conditions. Choices A, C, and D are incorrect because the bacterial flagellum is not involved in protein synthesis, DNA replication, or spore formation. Protein synthesis occurs in ribosomes, DNA replication takes place in the nucleus or nucleoid, and spore formation is a specialized process involving the production of spores for survival in harsh conditions.

Correct Answer: A

Rationale: The correct answer is A: T-lymphocytes. Cell-mediated immunity involves the activation of T-lymphocytes, which play a crucial role in recognizing and killing infected cells. T-lymphocytes include cytotoxic T cells, helper T cells, and regulatory T cells, all of which are involved in different aspects of cell-mediated immunity. Monocytes (choice B) are a type of white blood cell involved in phagocytosis but are not the main mediators of cell-mediated immunity. B-lymphocytes (choice C) are involved in humoral immunity, producing antibodies, not cell-mediated immunity. Choice D (A+B+C) is incorrect because cell-mediated immunity is mainly mediated by T-lymphocytes.

Correct Answer: A

Rationale: The correct answer is A, Clostridium botulinum. This bacterium produces botulinum toxin, leading to botulism. It is commonly found in soil and improperly canned food. Clostridium tetani causes tetanus, Staphylococcus aureus causes food poisoning, and Salmonella enterica causes salmonellosis. Therefore, A is correct due to its association with botulism, while the other choices are incorrect for causing different diseases.

Correct Answer: D

Rationale: The correct answer is D, Salmonella typhi, because it is a motile bacterium due to possessing flagella that aid in movement. Bacillus anthracis (A) is non-motile, Klebsiella pneumoniae (B) lacks flagella for motility, and Shigella flexneri (C) is non-motile as well. Salmonella typhi (D) is known for its flagella-driven motility, allowing it to move and spread within its host and environment. Flagella are essential for bacterial motility, enabling them to navigate and colonize different environments efficiently.

Correct Answer: A

Rationale: Aminoglycosides primarily inhibit protein synthesis by binding to the 30S ribosomal subunit in bacteria. This action leads to misreading of mRNA and ultimately disrupts the translation process, resulting in faulty protein production and bacterial cell death. Incorrect choices: B: Aminoglycosides do not inhibit DNA replication. C: Aminoglycosides do not disrupt cell membrane integrity. D: Aminoglycosides do not inhibit folic acid synthesis. In summary, the correct choice (A) aligns with the known mechanism of action of aminoglycosides, making it the most appropriate answer.