Ebola viruses need to be handled in which of the following biosafety levels?

Correct Answer: D

Rationale: The correct answer is D: BSL4. Ebola viruses are highly infectious and pose a high risk to laboratory workers. BSL4 facilities are designed to handle agents that pose a severe threat to human health and have no available vaccines or treatments. These facilities have strict guidelines for handling infectious agents, including full-body suits, negative air pressure, and decontamination processes to prevent any possible exposure. BSL1, BSL2, and BSL3 are not adequate for handling Ebola viruses due to their lower levels of containment and safety measures.

Correct Answer: A

Rationale: The correct answer is A: Leffler's method. In this staining method, gentian violet solution is used as the primary stain, followed by Lugol's solution as a mordant, 960 spiritus as a decolorizer, and water fuchsin as a counterstain. Gentian violet stains the organisms purple, Lugol's solution helps in fixing the dye, 960 spiritus decolorizes non-specifically, and water fuchsin acts as a counterstain to help visualize the bacteria. Leffler's method is specifically used for staining sputum smears in suspected cases of croupous pneumonia. Summary: B: Gram's staining uses crystal violet, iodine, alcohol, and safranin. It is mainly used for distinguishing between Gram-positive and Gram-negative bacteria. C: Ziehl-Neelsen's staining is used for acid-fast bacteria like Mycobacterium tuberculosis using carbol fuchsin and acid-alcohol

Correct Answer: D

Rationale: The correct answer is D: Fifth disease. Rubella virus is associated with Fifth disease, also known as erythema infectiosum. It is caused by parvovirus B19, not rubella virus. Mumps (choice A) is caused by the mumps virus. Roseola (choice B) is caused by human herpesvirus 6 or 7. Rubeola (choice C) is the scientific name for measles, caused by the measles virus. Therefore, the correct answer is Fifth disease as it is the disease associated with rubella virus.

Correct Answer: B

Rationale: The correct answer is B: genotype. The genotype refers to the actual genetic makeup of an organism, including all the genes and alleles present in its DNA. This determines the traits and characteristics of an organism. Phenotype (A) is the observable physical or biochemical characteristics of an organism. Conjugation (C) is a process in bacteria involving the transfer of genetic material. Construct (D) is not a term related to genetics. Therefore, the correct choice is B as it specifically refers to the genetic composition of an organism.

Correct Answer: B

Rationale: Step 1: Gram-negative, comma-shaped bacterium suggests a curved rod morphology, typical of Campylobacter jejuni. Step 2: Motility is a characteristic of Campylobacter species. Step 3: Campylobacter jejuni is a common cause of bacterial diarrhea in humans. Step 4: Vibrio cholerae causes cholera, not typically associated with comma-shaped morphology. Shigella dysenteriae and Escherichia coli are not comma-shaped.

Correct Answer: A

Rationale: The correct answer is A: Candida fungi. Candida fungi are known to cause oral thrush, characterized by whitish spots on the oral mucosa. The presence of gram-positive oval budding cells in the smear preparations is indicative of Candida species. Staphylococci (choice B) are gram-positive cocci, not oval budding cells. Sarcinae (choice C) are gram-positive bacteria that form cuboidal packets, not oval budding cells. Actinomycetes (choice D) are filamentous gram-positive bacteria, not oval budding cells like Candida fungi.

Correct Answer: A

Rationale: The correct answer is A: Bacteria. Urinary tract infections (UTIs) are primarily caused by the invasion of bacteria into the urinary tract. Bacteria, especially Escherichia coli (E. coli), are the most common culprits. They enter the urinary tract through the urethra and can multiply in the bladder, leading to infection. Viruses (choice B) and fungi (choice C) are not typical causes of UTIs. "None of the above" (choice D) is incorrect as bacteria are indeed the main cause of UTIs.

Correct Answer: B

Rationale: The correct answer is B because antigenic drift is not caused by the exchange of genetic information between animal and human influenza viruses. Antigenic drift is actually due to point mutations in the genes that encode for the surface proteins of the virus, leading to small changes in the antigens. These mutations can affect the hemagglutinin of both type A and B influenza viruses, making choices A and C correct. Choice D is incorrect because antigenic drift can occur in both type A and B influenza viruses, with type B being less prone to drift compared to type A.

Correct Answer: C

Rationale: Step 1: The nucleocapsid is the structure containing the viral genome and the protein capsid. Step 2: The genome carries the genetic material of the virus. Step 3: The capsid is the protein coat that encloses and protects the viral genome. Step 4: Therefore, the correct answer is C: Genome and capsid. Summary: A: Capsid and spikes - Incorrect, spikes are not part of the nucleocapsid. B: Genome and envelop - Incorrect, the envelop is not part of the nucleocapsid. D: Capsid and envelope - Incorrect, the envelope is not part of the nucleocapsid.